Handheld Multifunctional Fluorescence Imager for Non-invasive Plant Phenotyping

Zhang, Ruochong; Koh, Sally Shuxian; Teo, Mark Ju Teng; Bi, Renzhe; Zhang, Shuyan; Dev, Kapil; Urano, Daisuke; Dinish, U. S.; Olivo, Malini

doi:10.3389/fpls.2022.822634

Cited by 6 publications

(12 citation statements)

References 31 publications

(35 reference statements)

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“…The leaves were measured for fresh weight and snap frozen in liquid nitrogen until biochemical processing was performed 36 . Subsequently, the mARI value was correlated to the anthocyanin amount obtained from conventional biochemical analysis.…”

Section: Methodsmentioning

confidence: 99%

Miniaturized Vis–NIR handheld spectrometer for non-invasive pigment quantification in agritech applications

Dinish,

Teng,

Xinhui

et al. 2023

Sci Rep

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Advanced precision agriculture requires the objective measurement of the structural and functional properties of plants. Biochemical profiles in leaves can differ depending on plant growing conditions. By quantitatively detecting these changes, farm production processes can be optimized to achieve high-yield, high-quality, and nutrient dense agricultural products. To enable the rapid and non-destructive detection on site, this study demonstrates the development of a new custom-designed portable handheld Vis–NIR spectrometer that collects leaf reflectance spectra, wirelessly transfers the spectral data through Bluetooth, and provides both raw spectral data and processed information. The spectrometer has two preprogramed methods: anthocyanin and chlorophyll quantification. Anthocyanin content of red and green lettuce estimated with the new spectrometer showed an excellent correlation coefficient of 0.84 with those determined by a destructive gold standard biochemical method. The differences in chlorophyll content were measured using leaf senescence as a case study. Chlorophyll Index calculated with the handheld spectrometer gradually decreased with leaf age as chlorophyll degrades during the process of senescence. The estimated chlorophyll values were highly correlated with those obtained from a commercial fluorescence-based chlorophyll meter with a correlation coefficient of 0.77. The developed portable handheld Vis–NIR spectrometer could be a simple, cost-effective, and easy to operate tool that can be used to non-invasively monitor plant pigment and nutrient content efficiently.

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Section: Methodsmentioning

confidence: 99%

Miniaturized Vis–NIR handheld spectrometer for non-invasive pigment quantification in agritech applications

Dinish,

Teng,

Xinhui

et al. 2023

Sci Rep

Self Cite

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show abstract

“…Single-color LEDs are also relatively inexpensive, consume less power, and have a long lifetime. The LEDs can be arranged in an array (11,13,47,48) or in single source configurations (10,40,49) based upon the measurements that are desired. If absorption measurements are needed the sample needs to be placed between the excitation source and the camera, while fluorescence emission measurements require the LEDs and camera to be on the same side of the object being imaged.…”

Section: Methodsmentioning

confidence: 99%

“…Fluorescence imaging can be used to measure several parameters related to plant physiology such as environmental stress, photosynthetic efficiency, nutrient deficiency, and disease (40)(41)(42)(43). By measuring these parameters, researchers and agriculturists can gain insights into the health and performance of the plant under different environmental conditions where early detection of diseases and pests can save crops and improve yield.…”

Section: Plant Health Imagingmentioning

confidence: 99%

“…Anthocyanins are pigments that give plants, including lettuce, a red, purple, or blue color and protect plants from harmful radiation. Using the anthocyanin index formula (40) we calculated the anthocyanin response of a lettuce imaged over several days in our openIVIS system (Fig 9). As expected, the anthocyanin index clearly changes over time as the lettuce leaf becomes dehydrated in the imaging system.…”

Section: Plant Health Imagingmentioning

confidence: 99%

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Multifunction Fluorescence Open-SourceIn Vivo/In VitroImaging System (openIVIS)

Branning,

Faughnan,

Tomson

et al. 2023

Preprint

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The widespread availability and diversity of open-source microcontrollers paired with off-the-shelf electronics and 3D printed technology has led to the creation of a wide range of low-cost scientific instruments, including microscopes, spectrometers, sensors, data loggers, and other tools that can be used for research, education, and experimentation. These devices can be used to explore a wide range of scientific topics, from biology and chemistry to physics and engineering. In this study we designed and built a multifunction fluorescent open-source in-vivo/in-vitro imaging system (openIVIS) fluorescent imaging system that integrates a Raspberry Pi with commercial cameras and LEDs with 3D printed structures combined with an acrylic housing. Our openIVIS provides three excitation wavelengths of 460 nm, 520 nm, and 630 nm integrated with Python control software to enable fluorescent measurements across the full visible light spectrum. To demonstrate the various potential applications of our system, we tested its performance against a diverse set of experiments including laboratory type assays (measuring fluorescent dyes, using optical nanosensors, and DNA gel electrophoresis) to potentially fieldable applications (plant and mineral imaging). We also tested the potential use for a high school biology environment by imaging small animals and tracking their development over the course of a couple of weeks. Our system demonstrated its ability to measure a wide dynamic range fluorescent response from millimolar to picomolar concentrations in the same sample while measuring responses across visible wavelengths. These results demonstrate the power and flexibility of open-source hardware and software and how it can be integrated with customizable manufacturing to create low-cost scientific instruments with a wide range of applications. Our study provides a promising model for the development of low-cost instruments that can be used in both research and education.

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“…The leaves were measured for fresh weight and snap frozen in liquid nitrogen until biochemical processing was performed 24 . Subsequently, the mARI value was correlated to the anthocyanin amount obtained from conventional biochemical analysis.…”

Section: Anthocyanin Quanti Cationmentioning

confidence: 99%

Miniaturized Vis-NIR handheld spectrometer for non-invasive plant pigment quantification

Teo

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Advanced precision agriculture requires the objective measurement of the structural and functional properties of plants. Biochemical profiles in leaves can differ depending on plant growing conditions. By quantitatively detecting these changes, farm production processes can be optimized to achieve high-yield, high-quality, and nutrient dense agricultural products. To enable the rapid and non-destructive detection on site, this study demonstrates the development of a new custom-designed portable handheld Vis-NIR spectrometer that collects leaf reflectance spectra, wirelessly transfers the spectral data through Bluetooth, and provides both raw spectral data and processed information. The spectrometer has two preprogramed methods: anthocyanin and chlorophyll quantification. Anthocyanin content of red and green lettuce estimated with the new spectrometer showed an excellent correlation coefficient of 84% with those determined by a destructive gold standard biochemical method. The differences in chlorophyll content were measured using leaf senescence as a case study. Chlorophyll Index calculated with the handheld spectrometer gradually decreased with leaf age as chlorophyll degrades during the process of senescence. The estimated chlorophyll values were highly correlated with those obtained from a commercial fluorescence-based chlorophyll meter with a correlation coefficient of 77%. The developed portable handheld Vis-NIR spectrometer could be a simple, cost-effective, and easy to operate tool that can be used to non-invasively monitor plant pigment and nutrient content efficiently.

show abstract

Handheld Multifunctional Fluorescence Imager for Non-invasive Plant Phenotyping

Cited by 6 publications

References 31 publications

Miniaturized Vis–NIR handheld spectrometer for non-invasive pigment quantification in agritech applications

Miniaturized Vis–NIR handheld spectrometer for non-invasive pigment quantification in agritech applications

Multifunction Fluorescence Open-SourceIn Vivo/In VitroImaging System (openIVIS)

Miniaturized Vis-NIR handheld spectrometer for non-invasive plant pigment quantification

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