Handheld fluorometer for in-situ melamine detection via interference synthesis of dsDNA-templated copper nanoparticles

Kunnath, Radhika Nambannor; Venukumar, Aravind; Gorthi, Sai Siva

doi:10.1016/j.saa.2020.118304

Cited by 7 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The limits of detection (LODs) were determined to be 40 ng/mL for the Quantus TM fluorometer and 203 ng/mL for the developed fluorometer, which were impressively lower than those of the immunoturbidimetry method by 150-fold and 30-fold, respectively [ 35 ], showcasing the excellent sensitivity of both portable fluorometers for albumin detection. The developed fluorometer’s LOD was 5-fold higher than that of the commercial fluorometer due to some environmental light leakage during the onsite field test.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, commercial handheld fluorometers require the interpretation of measured fluorescence intensities and concentrations [ 31 , 32 ]. While several groups have developed low-cost fluorometer prototypes, these prototypes are still in the early stages and are not specifically designed for albuminuria quantification [ 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Pinrod,

Chawjiraphan,

Segkhoonthod

et al. 2023

Biosensors

View full text Add to dashboard Cite

This study presents the development of a portable fluorometer with a smartphone application designed to facilitate the early screening of chronic kidney and renal diseases by enabling the sensitive detection of urinary albumin. Utilizing a fluorescence-based aptasensor, the device achieved a linear calibration curve (0.001–1.5 mg/mL) with a linearity of up to 0.98022 and a detection limit of 0.203 µg/mL for human serum albumin (HSA). The analysis of 130 urine samples demonstrated comparable performance between this study’s fluorometer, a commercial fluorometer, and the standard automated method. These findings validate the feasibility of the portable fluorometer and aptasensor combination as a reliable instrument for the sensitive and specific measurement of HSA in urine samples. Moreover, the fluorometer’s portability offers potential applications in portable point-of-care testing, enhancing its utility in clinical settings for early disease screening.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Pinrod,

Chawjiraphan,

Segkhoonthod

et al. 2023

Biosensors

View full text Add to dashboard Cite

show abstract

“…Portable devices with specific components such as a photodiode, a heating element, and a design that blocks external light for reliable detection would be more suitable for field deployment. Many reported prototypes could be adapted for this application using inexpensive off-the-shelf components, 3-D printing, and open-source software, , with the potential to expand the capabilities of the biosensor to quantitative detection. Moreover, we believe that our strategy can be deployed using other formats, such as lateral flow assays, with minimal modification of the invading probe.…”

Section: Discussionmentioning

confidence: 99%

Allosteric Regulation of DNA Circuits Enables Minimal and Rapid Biosensors of Small Molecules

Rodríguez-Serrano

Hsing

2021

ACS Synth. Biol.

View full text Add to dashboard Cite

Detection of environmental pollutants is crucial to safeguard ecological and public health. Here, we report a modular biosensing approach for the detection of contaminants based on the regulation of a minimal DNA signal amplifier and transducer circuit by allosteric transcription factors and their cognate ligands. We leverage the competition between allosteric proteins and an endonuclease to modulate cascade toehold-mediated strand displacement reactions, which are triggered in the presence of specific effectors and sustained by the endonuclease. We built two optical biosensors for the detection of tetracyclines and macrolides in water using repressors TetR and MphR, respectively. We demonstrate that our minimal, fast, and single-step biosensors can successfully detect antibiotics in nanomolar levels and apply them to report the presence of spiked-in antibiotics in water samples in a matter of minutes, suggesting great potential for monitoring of water contaminants.

show abstract

“…Fluorescence is the most widely used strategy for the analysis of miRNA biomarkers. In recent years, portable fluorometers have attracted much research interest due to their potential to replace expensive laboratory equipment [40]. Lim and his colleagues reported a portable fluorometer for diagnosing early-stage AD by virtue of miRNAs in the blood as biomarkers (Figure 2C) [41].…”

Section: Detection Of Mirnas Based On Portable Fluorometersmentioning

confidence: 99%

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

et al. 2023

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of small noncoding RNAs that are approximately 22 nt in length and regulate gene expression post-transcriptionally. miRNAs play a vital role in both physiological and pathological processes and are regarded as promising biomarkers for cancer, cardiovascular diseases, neurodegenerative diseases, and so on. Accurate detection of miRNA expression level in clinical samples is important for miRNA-guided diagnostics. However, the common miRNA detection approaches like RNA sequencing, qRT-PCR, and miRNA microarray are performed in a professional laboratory with complex intermediate steps and are time-consuming and costly, challenging the miRNA-guided diagnostics. Hence, sensitive, highly specific, rapid, and easy-to-use detection of miRNAs is crucial for clinical diagnosis based on miRNAs. With the advantages of being specific, sensitive, efficient, cost-saving, and easy to operate, point-of-care testing (POCT) has been widely used in the detection of miRNAs. For the first time, we mainly focus on summarizing the research progress in POCT of miRNAs based on portable instruments and visual readout methods. As widely available pocket-size portable instruments and visual detection play important roles in POCT, we provide an all-sided discussion of the principles of these methods and their main limitations and challenges, in order to provide a guide for the development of more accurate, specific, and sensitive POCT methods for miRNA detection.

show abstract

Handheld fluorometer for in-situ melamine detection via interference synthesis of dsDNA-templated copper nanoparticles

Cited by 7 publications

References 29 publications

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Development of a High-Accuracy, Low-Cost, and Portable Fluorometer with Smartphone Application for the Detection of Urinary Albumin towards the Early Screening of Chronic Kidney and Renal Diseases

Allosteric Regulation of DNA Circuits Enables Minimal and Rapid Biosensors of Small Molecules

Advances in Point-of-Care Testing of microRNAs Based on Portable Instruments and Visual Detection

Contact Info

Product

Resources

About