Hand-held thermal-regulating fluorometer

Farmer, Andrew; Fries, David; Flannery, W.; Massini, John

doi:10.1063/1.2126812

Cited by 11 publications

(3 citation statements)

References 7 publications

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“…We have previously described a number of handheld devices that utilize real‐time nucleic acid sequence‐based amplification (RT‐NASBA) (Kedia et al. 2005; Farmer et al. 2005; Smith et al.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously described a number of handheld devices that utilize real-time nucleic acid sequence-based amplification (RT-NASBA) (Kedia et al 2005;Farmer et al 2005;Smith et al 2007); however, these systems have relied on Boom extraction-based silica centrifugation columns for RNA extraction (Boom et al 1990). Here, we describe a more field-amenable RNA extraction technique that uses the ChargeSwitch (Invitrogen, Carlsbad, CA) magnetic separation protocol to purify total RNA from environmental samples.…”

Section: Introductionmentioning

confidence: 99%

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A Field‐able Rna Extraction and Purification Procedure

Smith

Fries

2008

Rapid Methods Automation Mic

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This study describes the use of the ChargeSwitch extraction technique as a method amenable to in‐field RNA extraction and purification. The modified ChargeSwitch extractions were performed by lysing cells captured from filtration of 30 mL seawater sample spiked with cultured Karenia brevis and seawater samples from regions around a known red tide event. Extractions were compared to traditional laboratory‐based silica spin columns by integration with real‐time nucleic acid sequence‐based amplification. The ChargeSwitch extraction method was equivalent to or was faster than traditional laboratory analysis whilst having the additional advantages of obtaining consistently higher RNA concentrations and requiring no specialized or powered equipment. This method represents a fast and field‐able method for RNA extraction from K. brevis and potentially other organisms of public health significance. PRACTICAL APPLICATIONS This manuscript describes an RNA extraction method that utilizes ChargeSwitch technology suited for in‐field use that can be performed without access to extensive laboratory infrastructure, requiring only magnetic separation equipment and adjustable pipettes. This research is both timely and important as a recent Alliance for Coastal Technologies' workshop on “Genetic Sensors for Environmental Water Quality” (January 5–7, 2005) highlighted in‐field sample preparation as one of the major issues facing the advancement of in situ genetic assays. The method uses detection of the harmful, algal bloom‐forming dinoflagellate Karenia brevis as a demonstration of the use of ChargeSwitch to extract RNA from complex environmental samples in the field. Combined with previously described portable analytical equipment, these techniques will prove invaluable in the advancement of on‐site analysis, leading to faster decision making for public health officials and shellfish managers.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Field‐able Rna Extraction and Purification Procedure

Smith

Fries

2008

Rapid Methods Automation Mic

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“…Farmer e colaboradores84 construíram um fluorímetro com sistema de regulagem de temperatura. O equipamento usa LED como fonte de radiação e este pode ser trocado de acordo com o analito a ser excitado.…”

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