Hamster and Murine Models of Severe Destructive Lyme Arthritis

Munson, Erik; Nardelli, Dean T.; Chateau, Brian K. Du; Callister, Steven M.; Schell, Ronald F.

doi:10.1155/2012/504215

Cited by 12 publications

(6 citation statements)

References 72 publications

(65 reference statements)

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“…A Syrian hamster model to study Borrelia burgdorferi infection was established by Johnson et al (144). After Borrelia burgodrferi infection, hamsters were utilized to study articular manifestations of Lyme borreliosis, which is similar to human (145). Syrian hamster can be successfully infected with Leishmania panamensis (146).…”

Section: Syrian Hamster Used For Research In Bacterial and Parasitic mentioning

confidence: 99%

Syrian Hamster as an Animal Model for the Study on Infectious Diseases

et al. 2019

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Infectious diseases still remain one of the biggest challenges for human health. In order to gain a better understanding of the pathogenesis of infectious diseases and develop effective diagnostic tools, therapeutic agents, and preventive vaccines, a suitable animal model which can represent the characteristics of infectious is required. The Syrian hamster immune responses to infectious pathogens are similar to humans and as such, this model is advantageous for studying pathogenesis of infection including post-bacterial, viral and parasitic pathogens, along with assessing the efficacy and interactions of medications and vaccines for those pathogens. This review summarizes the current status of Syrian hamster models and their use for understanding the underlying mechanisms of pathogen infection, in addition to their use as a drug discovery platform and provides a strong rationale for the selection of Syrian hamster as animal models in biomedical research. The challenges of using Syrian hamster as an alternative animal model for the research of infectious diseases are also addressed.

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Section: Syrian Hamster Used For Research In Bacterial and Parasitic mentioning

confidence: 99%

Syrian Hamster as an Animal Model for the Study on Infectious Diseases

et al. 2019

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“…The C3H/HeN mouse is the most frequently used animal strain to investigate the immune mechanisms of Lyme arthritis. The model has the advantages of mimicking the early innate immune response that occurs when the bacteria enter the host when transmitted by a vector [ 34 ], and using C3H/HeN mice is a promising model for studying the inflammation of subacute Lyme arthritis as a response to spirochetes localized in the joint tissue [ 35 ]. This allow us to test our 64 Cu-DOTATATE PET tracer as an early marker for the bacterial infection.…”

Section: Discussionmentioning

confidence: 99%

64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

et al. 2020

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Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato (Bb) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22luc. Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early (p < 0.0001) and late time points (p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.

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“…burgdorferi in the brain. While much progress has been made in investigating the immune response to Lyme disease using animal models, including mouse (Bramwell et al, 2014; Byram et al, 2015; Hansen et al, 2016; Sallay et al, 2017), hamster (Croke et al, 2000; Munson et al, 2012), rabbit (Shang et al, 2000; Scott et al, 2017), and dog (Smith et al, 2012; Wagner et al, 2015), these experimental animals can exhibit erythema migrans or arthritis. However, the rhesus macaque model plays an indispensable role in studies related to neuroborreliosis (Roberts et al, 1998; Pachner et al, 2001), as the overall understanding of the inflammation response leading to LNB remains elusive.…”

Section: Introductionmentioning

confidence: 99%

Rhesus Brain Transcriptomic Landscape in an ex vivo Model of the Interaction of Live Borrelia Burgdorferi With Frontal Cortex Tissue Explants

Ding

Tao

et al. 2019

Front. Neurosci.

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Lyme neuroborreliosis (LNB) is the most dangerous manifestation of Lyme disease caused by the spirochete Borrelia burgdorferi which can reach the central nervous system most commonly presenting with lymphocytic meningitis; however, the molecular basis for neuroborreliosis is still poorly understood. We incubated explants from the frontal cortex of three rhesus brains with medium alone or medium with added live Borrelia burgdorferi for 6, 12, and 24 h and isolated RNA from each group was used for RNA sequencing with further bioinformatic analysis. Transcriptomic differences between the ex vivo model of live Borrelia burgdorferi with rhesus frontal cortex tissue explants and the controls during the progression of the infection were identified. A total of 2249, 1064, and 420 genes were significantly altered, of which 80.7, 52.9, and 19.8% were upregulated and 19.3, 47.1, 80.2% were downregulated at 6, 12, and 24 h, respectively. Gene ontology and KEGG pathway analyses revealed various pathways related to immune and inflammatory responses during the spirochete infection were enriched which is suggested to have a causal role in the pathogenesis of neurological Lyme disease. Moreover, we propose that the overexpressed FOLR2 which was demonstrated by the real-time PCR and western blotting could play a key role in neuroinflammation of the neuroborreliosis based on PPI analysis for the first time. To our knowledge, this is the first study to provide comprehensive information regarding the transcriptomic signatures that occur in the frontal cortex of the brain upon exposure to Borrelia burgdorferi , and suggest that FOLR2 is a promising target that is associated with neuroinflammation and may represent a new diagnostic or therapeutic marker in LNB.

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Hamster and Murine Models of Severe Destructive Lyme Arthritis

Cited by 12 publications

References 72 publications

Syrian Hamster as an Animal Model for the Study on Infectious Diseases

Syrian Hamster as an Animal Model for the Study on Infectious Diseases

64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

Rhesus Brain Transcriptomic Landscape in an ex vivo Model of the Interaction of Live Borrelia Burgdorferi With Frontal Cortex Tissue Explants

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