Halotolerant rhizobacteria mitigate the effects of salinity stress on maize growth by secreting exopolysaccharides

Liu, Xiaoting; Chai, Jiali; Zhang, Yincui; Zhang, Chen; Lei, Yang; Li, Qingpu; Yao, Tuo

doi:10.1016/j.envexpbot.2022.105098

Cited by 24 publications

(13 citation statements)

References 57 publications

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Section: Introductionmentioning

confidence: 91%

“…We isolated a strain of Pseudomonas simiae MHR6, with high EPS production in our previous study. The important role of the EPS produced by P. simiae MHR6 in alleviating plant salt stress has already been confirmed, while the specific mechanism by which EPS alleviates plant salt stress has not yet been elucidated. Therefore, it is necessary to further study the impact of EPS on Na + adsorption by P. simiae MHR6, determine how different components of EPS affect the accumulation and tolerance of Na + and clarify the role of EPS in Na + adsorption strategies.…”

Section: Introductionmentioning

confidence: 99%

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Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress

Liu,

Yao,

Chai

et al. 2023

J. Agric. Food Chem.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress

Liu,

Yao,

Chai

et al. 2023

J. Agric. Food Chem.

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“…It has been extensively documented that increased EPS synthesis helps alleviate the detrimental effects of salinity on plants, resulting in improved plant growth under such conditions [19,47,48]. Previous studies on P. putida KT2440 have reported that mutants unable to form biofilms tend to overproduce EPS as a compensatory mechanism [24].…”

Section: Strainsmentioning

confidence: 99%

Importance of biofilm formation for promoting plant growth under salt stress in Pseudomonas putida KT2440

et al. 2023

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An underutilized experimental design was employed to isolate adapted mutants of the model bacterium Pseudomonas putida KT2440. The design involved subjecting a random pool of mini‐Tn5 mutants of P. putida KT2440 to multiple rounds of selection in the rhizosphere of soybean plants irrigated with a NaCl solution. The isolated adapted mutants, referred to as MutAd, exhibited a mutation in the gene responsible for encoding the membrane‐binding protein LapA, which plays a role in the initial stages of biofilm formation on abiotic surfaces. Two MutAd bacteria, MutAd160 and MutAd185, along with a lapA deletion mutant, were selected for further investigation to examine the impact of this gene on salt tolerance, rhizosphere fitness, production of extracellular polymeric substances (EPS), and promotion of plant growth. Despite the mutants’ inability to form biofilms, they were able to attach to soybean seeds and roots. The MutAd bacteria demonstrated an elevated production of EPS when cultivated under saline conditions, which likely compensated for the absence of biofilm formation. MutAd185 bacteria exhibited enhanced root attachment and promoted the growth of soybean plants in slightly saline soils. The proposed experimental design holds promise for expediting bacterial adaptation to the rhizosphere of plants under specific environmental conditions, identifying genetic mutations that enhance bacterial fitness in those conditions, and thereby increasing their capacity to promote plant growth.

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“…sp. MHR6, an EPS-producing bacterium, has been shown to decrease the adverse effects of salinity stress on plant growth by enhancing seedling growth, antioxidant levels, the osmotic regulator, and chlorophyll accumulation ( Liu et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharide-producing bacteria enhanced Pb immobilization and influenced the microbiome composition in rhizosphere soil of pakchoi (Brassica chinensis L.)

Cao

Zhang²,

Ju³

et al. 2023

Front. Microbiol.

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Lead (Pb) contamination of planting soils is increasingly serious, leading to harmful effects on soil microflora and food safety. Exopolysaccharides (EPSs) are carbohydrate polymers produced and secreted by microorganisms, which are efficient biosorbent materials and has been widely used in wastewater treatment to remove heavy metals. However, the effects and underlying mechanism of EPS-producing marine bacteria on soil metal immobilization, plant growth and health remain unclear. The potential of Pseudoalteromonas agarivorans Hao 2018, a high EPS-producing marine bacterium, to produce EPS in soil filtrate, immobilize Pb, and inhibit its uptake by pakchoi (Brassica chinensis L.) was studied in this work. The effects of strain Hao 2018 on the biomass, quality, and rhizospheric soil bacterial community of pakchoi in Pb-contaminated soil were further investigated. The results showed that Hao 2018 reduced the Pb concentration in soil filtrate (16%–75%), and its EPS production increased in the presence of Pb2+. When compared to the control, Hao 2018 remarkably enhanced pakchoi biomass (10.3%–14.3%), decreased Pb content in edible tissues (14.5%–39.2%) and roots (41.3%–41.9%), and reduced the available Pb content (34.8%–38.1%) in the Pb-contaminated soil. Inoculation with Hao 2018 raised the pH of the soil, the activity of several enzymes (alkaline phosphatase, urease, and dehydrogenase), the nitrogen content (NH4+-N and NO3−-N), and the pakchoi quality (Vc and soluble protein content), while also raising the relative abundance of bacteria that promote plant growth and immobilize metals, such as Streptomyces and Sphingomonas. In conclusion, Hao 2018 reduced the available Pb in soil and pakchoi Pb absorption by increasing the pH and activity of multiple enzymes and regulating microbiome composition in rhizospheric soil.

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Halotolerant rhizobacteria mitigate the effects of salinity stress on maize growth by secreting exopolysaccharides

Cited by 24 publications

References 57 publications

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress

Importance of biofilm formation for promoting plant growth under salt stress in Pseudomonas putida KT2440

Exopolysaccharide-producing bacteria enhanced Pb immobilization and influenced the microbiome composition in rhizosphere soil of pakchoi (Brassica chinensis L.)

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Halotolerant rhizobacteria mitigate the effects of salinity stress on maize growth by secreting exopolysaccharides

Cited by 24 publications

References 57 publications

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na+/K+ Homeostasis in Maize under Salt Stress

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na+/K+ Homeostasis in Maize under Salt Stress

Importance of biofilm formation for promoting plant growth under salt stress in Pseudomonas putida KT2440

Exopolysaccharide-producing bacteria enhanced Pb immobilization and influenced the microbiome composition in rhizosphere soil of pakchoi (Brassica chinensis L.)

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Product

Resources

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Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress

Adsorption of Sodium Ions by Exopolysaccharides from Pseudomonas simiae MHR6 and Its Improvement of Na⁺/K⁺ Homeostasis in Maize under Salt Stress