Halotolerant and Extremely Halophilic Oil-Oxidizing Bacteria in Oil Fields

Belyaev, S. S.; Borzenkov, I. A.; Milekhina, E. I.; Zvyagintseva, I. S.; Ivanov, Mikhail

doi:10.1016/s0376-7361(09)70051-2

Cited by 7 publications

(3 citation statements)

References 1 publication

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“…Anaerobic and catalase tests show that all strains were able to grow under anaerobic condition and are catalase positive. These findings are in good agreement with previous studies which reported that aerobic microorganisms present in well saline are predominated by gram-positive, facultative and spore forming rod bacteria [12]. Based on the biochemical and morphological tests (Table 1) done according to the Bergey's manual [13], all strains belonged to the genus Bacillus.…”

Section: Isolation and Characterization Of Microorganismssupporting

confidence: 90%

“…All the other strains were also capable of growing on kerosene containing medium except for strain S7. Although most of reservoir microorganisms have been reported to have the ability to utilize hydrocarbon [12], some do not oxidize higher hydrocarbons but can only degrade low molecularweight hydrocarbon such as gases [7]. The ability to grow on hydrocarbons is widespread for bacteria such as Pseudomonas, Alcaligenes, Micrococcus, Norcadia, Bacillus, Rhodococcus and Proteus.…”

Section: Isolation and Characterization Of Microorganismsmentioning

confidence: 99%

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Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

Illias¹,

Ooi²,

Idris³

et al. 2012

Jurnal Teknologi

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Beberapa bakteria halotoleran telah berjaya dipencilkan daripada sampel air masin telaga minyak Semangkok di mana ujian biokimia dan morfologi telah dijalankan bagi bakteria ini. Hampir semua bakteria yang dipencilkan tergolong di dalam genus Bacillus. Sebahagian besar daripada pencilan berkeupayaan untuk hidup di dalam medium pertumbuhan yang mengandungi kerosen sebagai sumber karbon utama dan tenaga dan berkeupayaan untuk hidup di dalam medium dengan kepekatan NaCI diantara 10–15%. Ujian ketegangan antara permukaan (IFT) menunjukkan pencilan menghasilkan biosurfaktan. Enam daripada sembilan pencilan menghasilkan eksopolisakarida di dalam medium pertumbuhan yang mungkin penting di dalam MEOR. Kata kunci: Bakteria; pencirian; pengasingan; Surfaktan; Eksopolisakarida Several halotolerant bacteria were isolated from brine samples from Semangkok oil reservoir. Biochemical and morphological characterization of the bacteria were carried out. These bacteria are gram positive spore formers and have been identified as belonging to the genus Bacillus. Most of the isolates could grow in medium containing kerosene as sole carbon source and energy and tolerate NaCI concentration up to 15%. Interfacial tension and surface tension tests showed that the bacteria were capable of producing biosurfactant. Six out of nine were able to produce exopolysaccharide. We believe these isolate could be appointed as future biopolymer producer especially for microbial enhanced oil recovery (MEOR). Keywords: Bacteria; Characterization; Isolation; Surfactant; Exopolysaccharides.

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Section: Isolation and Characterization Of Microorganismssupporting

confidence: 90%

Section: Isolation and Characterization Of Microorganismsmentioning

confidence: 99%

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

Illias¹,

Ooi²,

Idris³

et al. 2012

Jurnal Teknologi

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“…There are only a few reports on the degradation of hydrocarbons in hypersaline environments. Enrichment cultures from the Great Solar Lake grew on mineral oil at salinities up to 17.2% (70), and a bacterial consortium and an extremely halophilic archaeon grew on hexadecane and crude oil at salinities of 15% and 32%, respectively (5). Degradation of hydrocarbons (mainly n-alkanes) by archaea (Halobacteria) at 15 to 32% NaCl was also reported (40).…”

mentioning

confidence: 98%

Population Dynamics within a Microbial Consortium during Growth on Diesel Fuel in Saline Environments

Kleinsteuber

Riis

Fetzer

et al. 2006

Appl Environ Microbiol

134

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The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% NaCl were monitored by singlestrand conformation polymorphism community fingerprinting of the PCR-amplified V4-V5 region of the 16S rRNA genes. Information obtained by this qualitative approach was extended by flow cytometric analysis to follow quantitatively the dynamics of community structures at different salinities. Dominant and newly developing clusters of individuals visualized via their DNA patterns versus cell sizes were used to identify the subcommunities primarily involved in the degradation process. To determine the most active species, subcommunities were separated physically by high-resolution cell sorting and subsequent phylogenetic identification by 16S rRNA gene sequencing. Reduced salinity favored the dominance of Sphingomonas spp., whereas at elevated salinities, Ralstonia spp. and a number of halophilic genera, including Halomonas, Dietzia, and Alcanivorax, were identified. The combination of cytometric sorting with molecular characterization allowed us to monitor community adaptation and to identify active and proliferating subcommunities.Many oil fields are located in semiarid regions characterized by soils of high natural salinity. Careless operation during oil extraction and processing contaminates the soil, for instance, via the generation of large volumes of oily and saline wastewater. Strategies are thus needed to effectively treat oil pollution of highly saline waters and soils.The degradation of hydrocarbons as major components of mineral oil is generally brought about by microorganisms. At the salinity of seawater (ϳ3% total salts, mainly NaCl) or below, most low-molecular-weight components of mineral oil are easily degraded by a variety of microorganisms. However, the range of degrading organisms decreases with increasing salinity. There are only a few reports on the degradation of hydrocarbons in hypersaline environments. Enrichment cultures from the Great Solar Lake grew on mineral oil at salinities up to 17.2% (70), and a bacterial consortium and an extremely halophilic archaeon grew on hexadecane and crude oil at salinities of 15% and 32%, respectively (5). Degradation of hydrocarbons (mainly n-alkanes) by archaea (Halobacteria) at 15 to 32% NaCl was also reported (40). An extremely halophilic archaeon from a salt marsh degraded various saturated and aromatic hydrocarbons at salinities of up to 31% and grew optimally at 22% NaCl (10). In our own laboratory, microbial communities from saline soils in Patagonia degraded diesel fuel up to a salinity of 17.5% (59). Although some members of the communities were identified, their contributions to the diesel fuel biodegradation remained obscure.Information on the phylogenetic diversity of microbial communities can be obtained by molecular methods, like...

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Growth and hydrocarbon degradation by three desert fungi under conditions of simultaneous temperature and salt stress

Obuekwe

Badrudeen

Al-Saleh

et al. 2005

International Biodeterioration & Biodegradation

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Halotolerant and Extremely Halophilic Oil-Oxidizing Bacteria in Oil Fields

Cited by 7 publications

References 1 publication

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

Isolation and Characterization of Halotolerant Aerobic Bacteria From Oil Reservoir

Population Dynamics within a Microbial Consortium during Growth on Diesel Fuel in Saline Environments

Growth and hydrocarbon degradation by three desert fungi under conditions of simultaneous temperature and salt stress

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