Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern

Cui, Heng‐Lin; Gao, Xia; Yang, Xin; Xu, Xue-Wei

doi:10.1007/s00792-010-0329-0

Cited by 117 publications

(73 citation statements)

References 33 publications

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“…These reference strains were routinely grown aerobically at 37 u C in NHM medium. Polar lipids were extracted using a chloroform/methanol system and analysed using one-and two-dimensional TLC, as described previously (Cui et al, 2010). Merck silica gel 60 F 254 aluminium-backed thin-layer plates were used for TLC analyses.…”

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confidence: 99%

Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula

Zhang

Cui

2014

International Journal of Systematic and Evolutionary Microbiology

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Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula Halophilic archaeal strain YGHS32 T was isolated from the Yinggehai marine solar saltern near Shanya city of Hainan Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain YGHS32 T was able to grow at 20-50 6C (optimum 37 6C), in the presence of 0.9-4.8 M NaCl (optimum 2.1 M NaCl), with 0.005-1.0 M MgCl 2 (optimum 0.3 M MgCl 2 ) and at pH 6.0-8.5 (optimum pH 7.5). The minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and four major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, glucosyl mannosyl glucosyl diether and a diglycosyl diether. Strain YGHS32 T had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of Halomicroarcula pellucida JCM 17820 T (92.9-96.3 % sequence similarity). The rpoB9 gene sequence similarity between strain YGHS32 T and Halomicroarcula pellucida JCM 17820 T was 91.3 %. The DNA G+C content of strain YGHS32 T was 64.0 mol%. The DNA-DNA hybridization value between strain YGHS32 T and Halomicroarcula pellucida JCM 17820 T was 45 %. It was concluded that strain YGHS32 T (5CGMCC 1.12129 T 5JCM 18640 T ) represents a novel species of the genus Halomicroarcula, for which the name Halomicroarcula limicola sp. nov. is proposed. An emended description of the genus Halomicroarcula is also presented.

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Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula

Zhang

Cui

2014

International Journal of Systematic and Evolutionary Microbiology

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“…rarus (isolated from a marine solar saltern, China) [18], Hrs. ruber (isolated from a salt lake, China) [19] and Hrs.…”

Section: Order Halobacterialesmentioning

confidence: 99%

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

et al. 2017

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Halophilic archaea, also referred to as haloarchaea, dominate hypersaline environments. To survive under such extreme conditions, haloarchaea and their enzymes have evolved to function optimally in environments with high salt concentrations and, sometimes, with extreme pH and temperatures. These features make haloarchaea attractive sources of a wide variety of biotechnological products, such as hydrolytic enzymes, with numerous potential applications in biotechnology. The unique trait of haloarchaeal enzymes, haloenzymes, to sustain activity under hypersaline conditions has extended the range of already-available biocatalysts and industrial processes in which high salt concentrations inhibit the activity of regular enzymes. In addition to their halostable properties, haloenzymes can also withstand other conditions such as extreme pH and temperature. In spite of these benefits, the industrial potential of these natural catalysts remains largely unexplored, with only a few characterized extracellular hydrolases. Because of the applied impact of haloarchaea and their specific ability to live in the presence of high salt concentrations, studies on their systematics have intensified in recent years, identifying many new genera and species. This review summarizes the current status of the haloarchaeal genera and species, and discusses the properties of haloenzymes and their potential industrial applications. SYSTEMATICS OF HALOPHILIC ARCHAEAHalophilic micro-organisms are found in the three domains of life, and are extremophiles living in hypersaline environments in the presence of salt (generally NaCl) as a specific requisite for their growth [1]. Hypersaline environments contain higher salt concentrations than seawater (3.5 % total dissolved salts) [2]. According to the optimum NaCl concentration for growth, micro-organisms fall into one of the following categories: extreme halophiles with optimum growth at 15-30 % (2.5-5.2 M) NaCl; moderate halophiles with optimum growth at 3-15 % (0.5-2.5 M) NaCl; slight halophiles with optimum growth at 1-3 % (0.2-0.5 M) NaCl; non-halophiles with optimum growth at less than 1 % (0.2 M) NaCl; and halotolerant micro-organisms which are non-halophiles showing the ability to tolerate high NaCl concentrations [3]. Halophiles can live in hypersaline environments because they are able to sustain osmotic balance. They use two main strategies to withstand the adverse consequences of water loss when exposed to high salt concentrations. The first strategy used by extremely halophilic archaea (haloarchaea) and halophilic anaerobic bacteria such as members of Halanaerobiales is known as the 'salt-in-cytoplasm' strategy, during which salts such as NaCl or KCl accumulate in the cytoplasm at concentrations similar to the extracellular ones [4,5]. Another mechanism to cope with high salt concentrations is the accumulation of organic molecules such as amino acids, sugars and polyols, known as compatible solutes or osmolytes. Compatible solutes are small and highly soluble molecules that can...

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“…Characterization of strain YJ-50-S2 T was performed according to the proposed minimal standards for description of new taxa (Oren et al, 1997) and as described previously by Cui et al (2010a). The analyses were conducted using NHM medium at 37 C unless otherwise noted.…”

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“…Halophilic archaeal polar lipids were extracted using a chloroform/methanol system and analysed using one-and two-dimensional TLC, as described previously (Cui et al, 2010a). Two specific detection spray reagents, phosphate stain reagent for phospholipids and anaphthol stain for glycolipids, were used.…”

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Salinigranum salinum sp. nov., isolated from a marine solar saltern

Wang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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An extremely halophilic archaeal strain YJ-50-S2 T was isolated from Yangjiang marine solar saltern, China. Cells were pleomorphic, stained Gram-negative and formed red-pigmented colonies on agar plates. Strain YJ-50-S2 T was able to grow at 25-50 C (optimum 37 C), with 0.9-4.8 M NaCl (optimum 2.6 M NaCl) and 0-1.0 M MgCl 2 (optimum 0.03 M MgCl 2 ), and at pH 5.0-9.5 (optimum pH 7.5). The cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The 16S rRNA gene and rpoB¢ gene of strain YJ-50-S2 T were phylogenetically related to the corresponding genes of Salinigranum rubrum GX10 T (97.0 % and 90.5 % similarities, respectively). The major polar lipids of strainYJ-50-S2 T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. Several unidentified glycolipids were also detected. The DNA G+C content of strain YJ-50-S2 T was 65.2 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-50-S2 T represents a novel species of the genus Salinigranum, for which the name Salinigranum salinum sp. nov. is proposed. The type strain is

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Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern

Cited by 117 publications

References 33 publications

Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula

Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

Salinigranum salinum sp. nov., isolated from a marine solar saltern

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