Halomonas malpeensis sp. nov., isolated from rhizosphere sand of a coastal sand dune plant

Abstract

A Gram-stain-negative, aerobic, non-endospore-forming organism, isolated from the rhizosphere sand of a coastal sand dune plant was studied for its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain YU-PRIM-29 was grouped within the genus Halomonas and was most closely related to Halomonas johnsoniae (97.5 %). The 16S rRNA gene sequence similarity to other Halomonas species was <97.5 %. Strain YU-PRIM-29 grew optimally at 28 °C (growth range, 10-36 °C), at a pH of 7-9 (growth range, pH 5.5-12.0) and in the presence of 0.5 to 5 % (w/v) NaCl (growth up to 20 % NaCl). The fatty acid profile from whole-cell hydrolysates supported the allocation of the strain to the genus Halomonas. The fatty acids C18 : 1ω7c and C16 : 0 were found as major compounds, followed by the hydroxylated fatty acid C12 : 0 3-OH. The quinone system consisted predominantly of ubiquinone Q-9. The polar lipid profile was composed of the major lipids diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In the polyamine pattern, spermidine was the predominant compound. The DNA G+C content was 64.8 mol%. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain YU-PRIM-29 from its closest-related species. Hence, YU-PRIM-29 represents a new species of the genus Halomonas, for which we propose the name Halomonas malpeensis sp. nov., with YU-PRIM-29 (=LMG 28855=CCM 8737) as the type strain.

Halomonas radicis sp. nov., isolated from Arthrocnemum macrostachyum growing in the Odiel marshes(Spain) and emended descriptions of Halomonas xinjiangensis and Halomonas zincidurans

“…Additionally, it was here got that strain EAR18 T neither hydrolyses aesculin nor gelatine ( Similar results were already described for other species within the genus [9,15,16,18,19,51] with the exception of urease activity and the capability to assimilate potassium gluconate which are so far only described for the isolate EAR18 T . Finally, strain EAR18 T was catalase positive and oxidase negative in line with other species in the genus [53].…”

“…Halomonas malpeensis and H. zincidurans in which the absence of growth was indicated on this medium [18,50]. Similar to other species described in the genus [51], it grew well on MA.…”

“…According to Ventosa et al [52] it could be therefore considered halotolerant in agreement with other species belonging to the genus [5,11,15,18,50].…”

“…At the time of writing, Halomonas comprises 94 species [6] being the type species Halomonas elongata [5]. Representatives of the genus have been mainly isolated from saline lakes [7,8], fermented seafood [9,10], saline soils/sediments [11,12,13,14,15], and marine environment [16,17], although recently they were also found associated with plant rhizosphere [18] and inhabiting plant stems [19]. Strain EAR18 T was an endophytic bacterium isolated from roots of an Arthrocnemum macrostachyum plant growing in the Odiel marshes (37º 13'N -6º 57'O) (Huelva, Spain) [20].…”

Potential of Microbial Diversity of Coastal Sand Dunes: Need for Exploration in Odisha Coast of India

“…Extreme halo-tolerant novel bacterial species named as Halomonas malpeensis sp. nov. has been recently isolated from the rhizosphere sand of a coastal sand dune plant which could grow in the presence of 0.5 to 5 % (w/v) NaCl (growth up to 20 % NaCl) and had a wide range of growth conditions [28].…”