Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium.

Romanenko, Lyudmila A.; Schümann, Peter; Rohde, Manfred; Mikhailov, Valery V.; Stackebrandt, Erko

doi:10.1099/00207713-52-5-1767

Cited by 22 publications

(7 citation statements)

References 31 publications

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“…Species of the genus Halomonas are metabolically very diverse and can grow between 1% and 26% salt, with optimum growth around 9% salt (Mata et al , 2002; Arahal et al , 2007). They were previously isolated from various saline and hypersaline environments, such as evaporation ponds (Baati et al , 2008), saline wetland (Martínez‐Cánovas et al , 2004), soil (Quillaguaman et al , 2004) and soda lake (Duckworth et al , 2000), but also from Antarctic lakes (Franzmann et al , 1987), paintings (Heyrman et al , 2002), sea ascidians (Romanenko et al , 2002) and a sea anemone (Xiao et al , 2009). Their presence has also been confirmed in association with Artemia from the Great Salt Lake (UT) by clone library analysis, but only in association with adult animals and not with encysted embryos (Riddle et al , 2007, 2008).…”

Section: Discussionmentioning

confidence: 99%

Bacteria associated with Artemia spp. along the salinity gradient of the solar salterns at Eilat (Israel)

Tkavc

Ausec

Oren

et al. 2011

FEMS Microbiology Ecology

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The crustacean genus Artemia naturally inhabits various saline and hypersaline environments and is the most frequently laboratory-hatched animal for live feed in mari- and aquaculture. Because of its high economic importance, Artemia-bacteria interactions were so far studied mostly in laboratory strains. In this study, we focused our attention on the Artemia-associated microbiota in its natural environment in the solar salterns of Eilat, Israel. We applied a culture-independent method (clone libraries) to investigate the bacterial community structure associated with Artemia in five evaporation ponds with salinities from slightly above seawater (5%) to the point of saturation (32%), in two different developmental stages: in nauplii and in the intestine of adult animals. Bacteria found in naupliar and adult stages were classified within the Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Cyanobacteria. The halophilic proteobacterial genera Halomonas spp. and Salinivibrio spp. dominated the Artemia microbiota in both stages in all ponds. We also analysed a clone library of entire adult animals, revealing a novel bacterial phylogenetic lineage. This is the first molecular study of bacteria associated with two developmental stages of Artemia along a salinity gradient.

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Section: Discussionmentioning

confidence: 99%

Bacteria associated with Artemia spp. along the salinity gradient of the solar salterns at Eilat (Israel)

Tkavc

Ausec

Oren

et al. 2011

FEMS Microbiology Ecology

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“…The genus Halomonas is the largest genus in the family Halomonadaceae and was first proposed by Vreeland et al (1980). Species of the genus Halomonas have been isolated from diverse saline environments such as salterns, saline soils, estuarine water, salt lakes, deep sea, seafood and marine invertebrates (Vreeland et al , 1980; Ventosa et al , 1998; Romanenko et al , 2002; Yoon et al , 2002; Kaye et al , 2004; Lim et al , 2004; Arahal & Ventosa, 2006; Arenas et al , 2009; Guan et al , 2010; Guzmán et al , 2010; Kim et al , 2010; Wang et al , 2012; Jeong et al , 2013). At the time of writing, the genus Halomonas comprised 90 recognized species (Parte, 2015).…”

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confidence: 99%

Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

Lee

Kim

Yun

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, halotolerant and alkalitolerant bacterium, designated strain BH103 T , was isolated from saltern soil in Gomso, Korea. Cells of strain BH103 T were strictly aerobic, motile, straight rods and grew at pH 7.0-10.8 (optimum, pH 8.5), at 10-55 8C (optimum, 28 8C) and at salinities of 0-23 % (w/v) NaCl (optimum, 14 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH103 T belongs to the genus Halomonas, showing highest sequence similarity to Halomonas boliviensis LC1 T (97.7 %), Halomonas neptunia Eplume1 T (97.7 %), Halomonas variabilis III T (97.7 %), Halomonas alkaliantarctica CRSS T (97.7 %), Halomonas olivaria TYRC17 T (97.5 %), Halomonas titanicae BH1 T (97.2 %) and Halomonas sulfidaeris Esulfide1 T (96.2 %). The predominant ubiquinone was Q-9. The major fatty acids were C 18 : 1 v7c, C 16 : 1 v7c and/or iso-C 15 : 0 2-OH, C 16 : 0 and C 12 : 0 3-OH. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and an unknown phospholipid. The DNA G+C content of this novel isolate was 54.7 mol%. DNA-DNA relatedness between strain BH103 T and H. boliviensis KACC 16615 T , H. neptunia KCTC 2888 T , H. variabilis KCTC 2889 T , H. alkaliantarctica KCTC 22844 T , H. olivaria DSM 19074 T , H. titanicae JCM 16411 T and H. sulfidaeris DSM 15722 T was 45, 41, 39, 32, 38, 45 and 35 %, respectively. On the basis of polyphasic analysis from this study, strain BH103 T represents a novel species of the genus Halomonas, for which the name Halomonas salicampi sp. nov. is proposed. The type strain is BH103 T (5KACC 17609 T 5NBRC 109914 T 5NCAIM B 02528 T ).

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“…The genus Halomonas belongs to the family Halomonadaceae within the Gammaproteobacteria and at the time of writing comprised 35 species that had been isolated mainly from saline or hypersaline environments (Arahal et al, 2002;Bouchotroch et al, 2001;Dobson & Franzmann, 1996;Garcia et al, 2004;Lee et al, 2005;Lim et al, 2004;Martínez-Cánovas et al, 2004;Martínez-Checa et al, 2005;Mata et al, 2002;Mormile et al, 1999;Quillaguaman et al, 2004;Romanenko et al, 2002;Ventosa et al, 1998;Vreeland et al, 1980;Yoon et al, 2001Yoon et al, , 2002 or from unusual habitats such as dry mural paintings (Heyrman et al, 2002) and deep-sea hydrothermal vents (Kaye et al, 2004). Phylogenetic analysis using the 16S and 23S rRNA gene sequences and phenotypic studies demonstrated that this genus is very heterogeneous (Arahal et al, 2002;Mata et al, 2002).…”

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confidence: 99%

Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico

Soto-Ramírez

Sánchez‐Porro

Rosas

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, short rod to oval-shaped bacterium (strain MW2a T ) was isolated from the surface of leaves of the black mangrove Avicennia germinans and subjected to a polyphasic taxonomic study. Strain MW2aT was moderately halophilic, growing at NaCl concentrations in the range 0-25 % (w/v) with optimum growth at 5 % (w/v) NaCl. Growth occurred at 12-40 6C (optimum, 30-35 6C) and at pH 5.0-9.0 (optimum, pH 7.0-8.0). Strain MW2a T was strictly aerobic.Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Halomonas. The closest relative was Halomonas marisflavi, with 98.6 % 16S rRNA gene sequence similarity. The DNA G+C content of strain MW2a T was 61.5 mol%, which is in the range of values for Halomonas species. DNA-DNA hybridization with H. marisflavi showed a relatedness of 42 % and lower values were obtained with respect to other related Halomonas species. The major fatty acids were C 16 : 0 , C 19 : 0 cyclo v8c, C 18 : 1 v7c and C 12 : 0 3-OH. Overall, the phenotypic, genotypic and phylogenetic results presented in this study demonstrate that strain MW2aT represents a novel species within the genus Halomonas. The name Halomonas avicenniae sp. nov. is proposed, with strain MW2a T (=CECT 7193 T =CCM 7396 T ) as the type strain.The genus Halomonas belongs to the family Halomonadaceae within the Gammaproteobacteria and at the time of writing comprised 35 species that had been isolated mainly from saline or hypersaline environments (Arahal et al., 2002;Bouchotroch et al., 2001;Dobson & Franzmann, 1996;Garcia et al., 2004;Lee et al., 2005; Lim et al., 2004; Martínez-Cánovas et al., 2004; Martínez-Checa et al., 2005;Mata et al., 2002; Mormile et al., 1999; Quillaguaman et al., 2004;Romanenko et al., 2002;Ventosa et al., 1998;Vreeland et al., 1980;Yoon et al., 2001Yoon et al., , 2002 or from unusual habitats such as dry mural paintings (Heyrman et al., 2002) and deep-sea hydrothermal vents (Kaye et al., 2004). Phylogenetic analysis using the 16S and 23S rRNA gene sequences and phenotypic studies demonstrated that this genus is very heterogeneous (Arahal et al., 2002;Mata et al., 2002). One distinct group is represented by a single species, Halomonas marisflavi (Yoon et al., 2001). To date, there have been no reports of the occurrence of members of this genus being associated with mangroves that thrive on saline habitats. Avicennia germinans is a type of mangrove that has developed mechanisms to tolerate high salt concentrations in unstable substrates, to obtain oxygen in almost anoxic sediments and to reproduce in harsh areas. One of these mechanisms consists of the secretion of salt crystals on the leaves when the concentration is in excess of that normally present in the vascular system (Lugo & Snedaker, 1975). In this study, we determined the taxonomic position of strain MW2a T , which was isolated from the surface of leaves of the black mangrove A. germinans during a microbial diversity study. As a result of our studies, the name Halomonas avicenniae sp. nov. is propose...

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Halomonas halocynthiae sp. nov., isolated from the marine ascidian Halocynthia aurantium.

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Cited by 22 publications

References 31 publications

Bacteria associated with Artemia spp. along the salinity gradient of the solar salterns at Eilat (Israel)

Bacteria associated with Artemia spp. along the salinity gradient of the solar salterns at Eilat (Israel)

Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil

Halomonas avicenniae sp. nov., isolated from the salty leaves of the black mangrove Avicennia germinans in Puerto Rico

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