Halomonas dongshanensis sp. nov., isolated from the seawater of Dongshan Island

Abstract

A taxonomic study was carried out on strain yzlin-01T, isolated from Dongshan Island seawater. The bacterium was Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, and motile by polar flagella. Growth was observed at temperatures of 10–40 °C, at salinities of 0.5–18 %, and at pH of 6–10. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain yzlin-01T belonged to the genus Halomonas , with the highest sequence similarity to Halomonas malpeensis YU-PRIM-29T (96.7 %), followed by Halomonas johnsoniae T68687T (96.4 %) and Halomonas gomseomensis M12T (96.4 %), and other species of the genus Halomonas (93.4–96.3 %). The ANI and digital DNA–DNA hybridization estimate values between strain yzlin-01T and the closest type strain Halomonas malpeensis YU-PRIM-29T were 77.44 and 21.6 %, respectively. The principal fatty acids were summed feature 8 (consisting of C18 : 1 ω7c and/or C18 : 1 ω6c; 55.7 %), C16 : 0 (20.6 %), C12 : 0 3-OH (6.8 %), summed feature 3 (consisting of C16 : 1 ω7c and/or C16 : 1 ω6c; 5.1 %). The G+C content of the chromosomal DNA was 60.0 mol %. The respiratory quinone was identified as Q-9 (100 %). Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, aminophospholipid, and three unidentified phospholipids were present. Combined genotypic and phenotypic data suggest that strain yzlin-01T represents a novel species within the genus Halomonas , for which the name Halomonas dongshanensis sp. nov. is proposed, with the type strain yzlin-01T (=GDMCC 1.3202T=KCTC 92467T).

Genomic attributes and characterization of novel exopolysaccharide-producing bacterium Halomonas piscis sp. nov. isolated from jeotgal

“…Halomonas , a genus initially discovered by Vreeland in solar salterns, was reported with the description of Halomonas elongate ( Vreeland et al, 1980 ). The genus Halomonas encompasses 121 species isolated from various saline environments such as saline lakes, salt flats, seawater, fermented fish sauce, and saline-alkali soils ( Poli et al, 2007 ; Jiang et al, 2014 ; Li et al, 2022 ; Yoo et al, 2022 ; Lin et al, 2023 ). These bacteria, characterized by Gram-stain-negative, halophilic, rod-shaped, and catalase-positive, can grow under aerobic or facultative anaerobic conditions and exhibit a wide range of physiological and metabolic features.…”