Halocynthiibacter halioticoli sp. nov., isolated from the viscera of abalone Haliotis discus hannai

Abstract

A Gram-stain-negative, rod-shaped, glide, non-agellated, and facultatively aerobic bacterial strain, designated as Z654 T , was isolated from the gut of abalone Haliotis discus hannai from Rongcheng, Shandong province, China. Cells are 0.2-0.8 µm in width and 0.7-3.4 µm in length. Cells grew best at 30°C (range, 15-37°C), pH 7 (range, 6.0-8.5) and NaCl concentration of 2.0% (w/v) (range, 1-10%).According to the phylogenetic analysis of 16S rRNA gene sequence, the strain belongs to the genus Halocynthiibacter and the most close strain is Halocynthiibacter arcticus KCTC 42129 T (97.12%). The genome size of strain Z654 T was 3296250 bp and the DNA G + C content was 54.2 mol%. The average nucleotide identity (ANI) scores and digital DNA-DNA hybridization (dDDH) scores with H. arcticus KCTC 42129 T was 70% and 14.6-18.2% respectively. The predominant quinone was Q-10 and the major fatty acids were C 18:0 , C 18:1 ω7c 11-methyl and summed future 8. The polar lipids were consisted of phosphatidylcholine, phosphatidylglycerol, unidenti ed aminolipid and unidentifed lipids. Based on the phenotypic, phylogenetic and chemotaxonomic data, strain Z654 T was considered to represent a novel species of the genus Halocynthiibacter, for which the name Halocynthiibacte halioticoli sp. nov., is proposed. The type strain is Z654 T (= MCCC 1H00503 T = KCTC 92003 T ).