Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia

Goh, Falicia; Leuko, Stefan; Allen, Michelle A.; Bowman, John P.; Kamekura, Masahiro; Neilan, Brett A.; Burns, Brendan P.

doi:10.1099/ijs.0.64180-0

Cited by 75 publications

(58 citation statements)

References 22 publications

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“…All 25 archaeal isolates showed a maximum of only 92% identity to Halobacterium NCIMB 718, an uncharacterized halophilic archaeon. One of these isolates was determined to be a new species belonging to the genus Halococcus (Goh et al, 2006). Bacteria belonging to the genera Bacillus, Salinivibrio, Halomonas, Marinobacter, Porphyrobacter, Idiomarina and two isolates with highest identity to the bacteria K2-13 and K3 were cultured from HPS2 (summarized in Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Although it is possible that archaea are present in concentrations in the seawater that are below the limits of detection of the methods employed here, it would seem that these halophilic organisms are specific to these biosedimentary structures (Goh et al, 2006). The role of archaea in stromatolites is not well understood, and thus it is difficult to assess their contributions.…”

Section: Estimated Coverage and Richnessmentioning

confidence: 93%

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Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay

et al. 2008

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The stromatolites at Shark Bay, Western Australia, are analogues of some of the oldest evidence of life on Earth. The aim of this study was to identify and spatially characterize the specific microbial communities associated with Shark Bay intertidal columnar stromatolites. Conventional culturing methods and construction of 16S rDNA clone libraries from community genomic DNA with both universal and specific PCR primers were employed. The estimated coverage, richness and diversity of stromatolite microbial populations were compared with earlier studies on these ecosystems. The estimated coverage for all clone libraries indicated that population coverage was comprehensive. Phylogenetic analyses of stromatolite and surrounding seawater sequences were performed in ARB with the Greengenes database of full-length non-chimaeric 16S rRNA genes. The communities identified exhibited extensive diversity. The most abundant sequences from the stromatolites were a-and c-proteobacteria (58%), whereas the cyanobacterial community was characterized by sequences related to the genera Euhalothece, Gloeocapsa, Gloeothece, Chroococcidiopsis, Dermocarpella, Acaryochloris, Geitlerinema and Schizothrix. All clones from the archaeal-specific clone libraries were related to the halophilic archaea; however, no archaeal sequence was identified from the surrounding seawater. Fluorescence in situ hybridization also revealed stromatolite surfaces to be dominated by unicellular cyanobacteria, in contrast to the sub-surface archaea and sulphate-reducing bacteria. This study is the first to compare the microbial composition of morphologically similar stromatolites over time and examine the spatial distribution of specific microorganismic groups in these intertidal structures and the surrounding seawater at Shark Bay. The results provide a platform for identifying the key microbial physiology groups and their potential roles in modern stromatolite morphogenesis and ecology.

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Section: Resultsmentioning

confidence: 99%

Section: Estimated Coverage and Richnessmentioning

confidence: 93%

Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay

et al. 2008

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“…Differential characteristics between strain BZ256 T and closely related genera within the order Halobacteriales Taxa: 1, strain BZ256 T ; 2, Halogeometricum; 3, Halobaculum; 4, Haloquadratum; 5, Halorubrum; 6, Haloferax; 7, Halococcus. Data for reference genera are from Burns et al (2007), Castillo et al (2006c), Cui et al (2006), Goh et al (2006), Grant et al (2001), Montalvo-Rodriguez et al (1998), Oren et al (1995) and Xu et al (2007). +, Positive; 2, negative; +/2, variable; NR, not reported.…”

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Halosarcina pallida gen. nov., sp. nov., a halophilic archaeon from a low-salt, sulfide-rich spring

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Krumholz

Oren

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Halosarcina pallida gen. nov., sp. nov., a halophilic archaeon from a low-salt, sulfide-rich spring A novel halophilic archaeon, strain BZ256 T , was isolated from Zodletone Spring, a sulfide-and sulfur-rich spring in south-western Oklahoma, USA. Cells were non-motile, non-flagellated cocci that divided along two axes, resulting in the formation of sarcina-like clusters. Strain BZ256 T grew at salt concentrations ranging from 1.3 to 4.3 M NaCl, with optimum growth at approximately 3.4 M, and required at least 1 mM Mg 2+ for growth. The pH range for growth was 5.0 to at least 8.5, and the temperature range for growth was 25-45 6C. The two diether phospholipids that are typical of members of the order Halobacteriales, namely phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, were present in strain BZ256 T , as were two glycolipids chromatographically identical to S-DGD-1 and DGD-1. The 16S rRNA gene sequence of strain BZ256 T showed 96.8 % similarity to that of the type strain of Halogeometricum borinquense, the closest recognized species within the order Halobacteriales. The DNA G+C content of strain BZ256 T was 65.4 mol%. Microscopic, physiological, biochemical and phylogenetic comparisons between strain BZ256 T and recognized genera of extremely halophilic archaea suggest that this strain represents a member of a novel genus and species within the family Halobacteriaceae, for which the name Halosarcina pallida gen. nov., sp. nov. is proposed. The type strain of Halosarcina pallida is BZ256 T (5KCTC 4017 T 5JCM 14848 T ).Members of the family Halobacteriaceae, the single family recognized within the order Halobacteriales, have long been identified as the most abundant micro-organisms in hypersaline environments (Oren, 1994). At the time of writing, the family Halobacteriaceae comprised 25 recognized genera and 101 recognized species. Recently, the extent of the family has been undergoing rapid expansion, with the description (as of June 2007) of five new genera and 20 novel species since 2006. This expansion has been due not only to the identification of novel taxa isolated from well-known hypersaline environments, such as the genera Halovivax, Halostagnicola, Haloplanus and Haloquadratum (Castillo et al., 2006a, b;Bardavid et al., 2007;Burns et al., 2007), but also to the recognition that members of the Halobacteriales can grow within saline microniches in non-saline environments at relatively low salt concentrations (Elshahed et al., 2004a;Savage et al., 2007). In addition, recent studies have reported the isolation of novel halobacterial strains (Purdy et al., 2004;Fukushima et al., 2007) or the detection of novel Halobacteriales-affiliated 16S rRNA gene sequences from moderate-to low-salinity systems (Munson et al., 1997;Takai et al., 2001;Walsh et al., 2005). These studies have shown that the order Halobacteriales is more diverse than previously believed. In the present study, we report on the isolation and characterization of strain BZ256 T from a sulfur-and sulfide-rich spring in south-...

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“…alococcus hamelensis was first isolated from living stromatolites in the hypersaline waters of Shark Bay, Australia (5). These ecosystems are analogous to one of the earliest forms of life on Earth (15) and exhibit significant microbial diversity (1,6,9,14).…”

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confidence: 99%

“…These ecosystems are analogous to one of the earliest forms of life on Earth (15) and exhibit significant microbial diversity (1,6,9,14). H. hamelinensis is the first archaeon isolated from stromatolites and displays a number of novel characteristics, including an oxidase-negative phenotype and lack of K ϩ uptake as a primary osmoprotective strategy (5,7). Although several key genes (uvrABC) involved in the adaptive response to UV stress have been identified (10), what is lacking is a comprehensive whole genomic profile and related computational analyses of this organism, which would significantly enhance our understanding of its evolutionary and adaptive traits.…”

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Genome Sequence of the Halophilic Archaeon Halococcus hamelinensis

2012

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Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia

Abstract: Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia

Cited by 75 publications

References 22 publications

Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay

Determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of Shark Bay

Halosarcina pallida gen. nov., sp. nov., a halophilic archaeon from a low-salt, sulfide-rich spring

Genome Sequence of the Halophilic Archaeon Halococcus hamelinensis

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