Halocin SH10 production by an extreme haloarchaeon Natrinema sp. BTSH10 isolated from salt pans of South India

Abstract

Halobacteria, members of the domain Archaea that live under extremely halophilic conditions, are often considered as dependable source for deriving novel enzymes, novel genes, bioactive compounds and other industrially important molecules. Protein antibiotics have potential for application as preserving agents in food industry, leather industry and in control of infectious bacteria. Halocins are proteinaceous antibiotics synthesized and released into the environment by extreme halophiles, a universal characteristic of halophilic bacteria. Herein, we report the production of halocin (SH10) by an extremely halophilic archeon Natrinema sp. BTSH10 isolated from salt pan of Kanyakumari, Tamilnadu, India and optimization of medium for enhanced production of halocin. It was found that the optimal conditions for maximal halocin production were 42 °C, pH 8.0, and 104 h of incubation at 200 rpm with 2% (V/V) inoculum concentration in Zobell's medium containing 3 M NaCl, Galactose, beef extract, and calcium chloride as additional supplements. Results indicated scope for fermentation production of halocin for probable applications using halophilic archeon Natrinema sp. BTSH10.

Isolation and Screening of Halophilic Bacteria for Production of Hydrolytic Enzymes

“…These have role to play as preserving agents in food and leather industries and in control of infectious bacteria (Torreblanca et al 1994Schiraldi et al 2002Karthikeyan et al 2013 ).…”

Antimicrobial peptides and proteins in the face of extremes: Lessons from archaeocins

“…No indication on the proteinaceous nature of the antimicrobial activity was mentioned. As for other halocins, the antimicrobial activity was maximal during the transition to the stationary phase of growth [55].…”

“…BTSH10, isolated from soil samples from salt pans of the Kanyakumari district (Tamilnadu, India), was selected for the strong antimicrobial activity of its supernatant against halophilic isolates from the saltern pond, and particularly against Halorubrum sp. BTSH3 [55]. Under phase contrast microscope photomicrographs, Halorubrum sp.…”

Chemical Oxygen Demand Removal in Na2SO4 Saturated Wastewater by Cultivable Halotolerant Bacterial Community

“…DH9‐1 reduced the COD removal effect when mixed with other strains. Some species of Natrinema and Actinopolyspora were reported to produce antibiotics which might be the reason of inhibition to other strains ; however, further research is needed to confirm this speculation. The chosen strains belonged to Actinobacteria , Proteobacteria , Firmicutes , and Bacteroidetes families according to systematic analysis by MEGA 5.0 software (Fig.…”