Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids

Abstract

Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95-97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58-64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.

Metagenomic analysis of the microbial community in Çal Cave soil to elucidate biotechnological potential

“…Halobacteria, which is an extremely halophilic archaea, produces biologically active compounds in response to environmental changes (UV radiation, temperature anomalies, oxidative stress, lack of nutrients, oxygen availability or dehydration) and to interaction with other microorganisms. These biologically active compounds are of widespread interest in several biotechnology industries (Kalenov et al, 2018). Sorokin et al, (2018) reported that strains from extremely halophilic Euryarchaea use insoluble celluloses, cellobiose, as their carbon and energy source.…”