Haloalkaliphilic Bacteria and Actinobacteria from the Saline Habitats: New Opportunities for Biocatalysis and Bioremediation

Singh, Satya P.; Raval, Vikram H.; Purohit, Megha K.; Thumar, Jignasha T.; Gohel, Sangeeta D.; Pandey, Sandip; Akbari, Viral G.; Rawal, C. M.

doi:10.1007/978-94-007-2229-3_19

Cited by 12 publications

(11 citation statements)

References 59 publications

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“…The actinobacterial sequences were assigned to the subclasses Actinobacteridae, Acidimicrobidae, Nitriliruptoridae, and Rubrobacteridae. More than half of actinobacterial sequences belonged to the order Actinomycetes, which is characterized by its mycelial growth, and with specific mechanisms of adapting to extreme environments, particularly saline and alkaline habitats (Singh et al 2012). Actinomycetes can grow on media containing 3.5 % NaCl (Maldonado et al 2005).…”

Section: Bacteriamentioning

confidence: 99%

“…Specific enzymes from soil halophiles are perceived to be potentially useful for a variety of applications, including the production of important biomolecules (Liszka et al 2012) and the remediation of pollutants in saline conditions (Dastgheib et al 2011). Soil halophiles are an excellent source for exploring novel enzymes possessing the inherent ability to function in high salt conditions (Singh et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In most cases only a few soil samples of a single specific saline system, or a small number of cloned sequences or DGGE bands, have been investigated and analyzed. Some studies have focused only one particular microbial group such as Archaea (Valenzuela-Encinas et al 2009;Youssef et al 2012) or Actinobacteria (Singh et al 2012). Numerous sequences recovered from saline soils are available from the GenBank, but many of these are largely overlooked because they are not published in the literature.…”

Section: Introductionmentioning

confidence: 99%

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A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

Gong

2013

World J Microbiol Biotechnol

100

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An integrated view of bacterial and archaeal diversity in saline soil habitats is essential for understanding the biological and ecological processes and exploiting potential of microbial resources from such environments. This study examined the collective bacterial and archaeal diversity in saline soils using a meta-analysis approach. All available 16S rDNA sequences recovered from saline soils were retrieved from publicly available databases and subjected to phylogenetic and statistical analyses. A total of 9,043 bacterial and 1,039 archaeal sequences, each longer than 250 bp, were examined. The bacterial sequences were assigned into 5,784 operational taxonomic units (OTUs, based on C97 % sequence identity), representing 24 known bacterial phyla, with Proteobacteria (44.9 %), Actinobacteria (12.3 %), Firmicutes (10.4 %), Acidobacteria (9.0 %), Bacteroidetes (6.8 %), and Chloroflexi (5.9 %) being predominant. Lysobacter (12.8 %) was the dominant bacterial genus in saline soils, followed by Sphingomonas (4.5 %), Halomonas (2.5 %), and Gemmatimonas (2.5 %). Archaeal sequences were assigned to 602 OTUs, primarily from the phyla Euryarchaeota (88.7 %) and Crenarchaeota (11.3 %).Halorubrum and Thermofilum were the dominant archaeal genera in saline soils. Rarefaction analysis indicated that less than 25 % of bacterial diversity, and approximately 50 % of archaeal diversity, in saline soil habitats has been sampled. This analysis of the global bacterial and archaeal diversity in saline soil habitats can guide future studies to further examine the microbial diversity of saline soils.

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Section: Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

Gong

2013

World J Microbiol Biotechnol

100

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“…Cloning and expression has become the most vital approach for culture independent enzyme discovery, exploration of this approach will generate biocatalysts with unique features. To extend our on-going work on the diversity, molecular phylogeny and enzymatic characterization of haloalkaliphilic bacteria from the saline habitats of Coastal Gujarat [9][10][11][12], in the present study, we successfully cloned, over-expressed and characterized an alkaline protease from the environmental DNA.…”

Section: Introductionmentioning

confidence: 99%

A metagenomic alkaline protease from saline habitat: Cloning, over-expression and functional attributes

Purohit

Singh

2013

International Journal of Biological Macromolecules

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“…aloalkaliphilic bacteria, a group of organisms with twin extremities of pH and salinity, have been investigated after isolation from a variety of habitats (2,3,4,5). The limited studies on the unique applications of extremophiles indicate that the unique "survival strategies" available for these organisms can be a great source of novel commercial applications.…”

mentioning

confidence: 99%

Whole-Genome Shotgun Sequencing of the Extremophile Alkalibacillus haloalkaliphilus C-5, of Indian Origin

Rawal¹,

Raval²,

Bhimani³

et al. 2012

Journal of Bacteriology

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Alkalibacillus haloalkaliphilus C-5 is a haloalkaliphilic bacterium that was isolated from a soil sample from the salty Sambhar Lake, Rajasthan, India. The organism is capable of alkaline protease production under conditions of pH 10 and 10% (wt/vol) salt. We sequenced and have reported the whole genome of Alkalibacillus haloalkaliphilus C-5, of Indian origin, for the first time.H aloalkaliphilic bacteria, a group of organisms with twin extremities of pH and salinity, have been investigated after isolation from a variety of habitats (2,3,4,5). The limited studies on the unique applications of extremophiles indicate that the unique "survival strategies" available for these organisms can be a great source of novel commercial applications. Their robust biocatalysts and unique metabolic capabilities are the major points of attractions of the organisms for developing processes for bioremediation and other biotechnological applications. Such important molecules from these microbes from unexplored and extreme habitats have recently prompted interest among scientific communities and industry (6). Most of the studies (4) on haloalkaliphilic bacteria, however, have so far focused on phylogenetic analyses of the organisms, and only limited information is available on their genomics and other potential biotechnology applications.Alkalibacillus haloalkaliphilus C-5 was isolated from a saline soil sample from Sambhar Lake, Rajasthan, India. It is an aerobic, Gram-positive, rod-shaped organism. It shows optimal growth at pH 10, 10% (wt/vol) NaCl, and 37°C. It produces a thermophilic serine protease. The genome sequence for Alkalibacillus haloalkaliphilus C-5 was determined to help us understand its physiology and enzymatic potentials.The whole-genome sequencing of Alkalibacillus haloalkaliphilus C-5 was performed using an Ion Torrent PGM sequencer. The data generated from the genomic library contained 419,990 reads and 46,141,879 nucleotide bases, with an average read length of 110 bases. Assembly using Newbler version 2.6 generated a 2,913,611-bp single chromosome. The genome annotation and comparative analysis of the genome were performed using the RAST program (rapid annotation using subsystem technology) (1). The organism has a GC content of 37.1%, with 3,160 predicted coding regions and 55 RNA genes. Of the 55 RNA genes, 52 code for tRNA, 2 for RNA, and 1 is for 5S RNA. For metabolic reconstruction, metabolic subsystems were assembled to create a metabolic reaction network for Alkalibacillus haloalkaliphilus C-5.The subsystem analysis revealed various genes involved in (i) metabolic pathways of cofactors, vitamins, prosthetic groups, and pigments, (ii) cell wall and capsule, (iii) virulence, disease, and defense, (iv) potassium metabolism, (v) membrane transport, (vi) iron acquisition and metabolism, (vii) RNA and protein metabolism, (viii) cell division and regulation, (ix) cell signaling, (x) DNA metabolism, (xi) nitrogen metabolism, (xii) sporulation, (xiii) stress responses, (xiv) metabolism of aromatic compounds, ami...

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Haloalkaliphilic Bacteria and Actinobacteria from the Saline Habitats: New Opportunities for Biocatalysis and Bioremediation

Cited by 12 publications

References 59 publications

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

A meta-analysis of the publicly available bacterial and archaeal sequence diversity in saline soils

A metagenomic alkaline protease from saline habitat: Cloning, over-expression and functional attributes

Whole-Genome Shotgun Sequencing of the Extremophile Alkalibacillus haloalkaliphilus C-5, of Indian Origin

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