Halo-fluorescein for photodynamic bacteria inactivation in extremely acidic conditions

Wang, Ying; Li, Jiazhuo; Zhou, Zhiwei; Zhou, Ronghui; Sun, Qun; Wu, Peng

doi:10.1038/s41467-020-20869-8

Cited by 42 publications

(24 citation statements)

References 46 publications

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“…In external HAE, photosensitizers are placed in a surrounding environment containing heavy atoms, such as a heavy atom-containing polymer matrix and halogenated solvents. , Although simple, its efficiency is extremely low, thus requiring a high concentration of heavy atoms to ensure a sufficient collision frequency between fluorophores and heavy atoms. On the contrary, internal HAE is achieved by covalently grafting of heavy atoms (e.g., Br and I) onto photosensitizers, − or employing Pt(II) or Ir(III) heavy-metal complexes as the photosensitizers. , Nevertheless, internal HAE often accompanies with complicated synthesis and purification procedures. Besides, neither external and internal HAE is selective, making controllable HAE modulation difficult.…”

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confidence: 99%

Selective Heavy Atom Effect Forming Photosensitizing Hot Spots in Double-Stranded DNA Matrix

Zhang

Liu

et al. 2021

J. Phys. Chem. Lett.

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Triplet exciton formation is essential for photosensitization-based photochemistry and photobiology. The heavy atom effect (HAE), in the form of either external or internal mode, is a basic mechanism for increasing the triplet exciton yield of photosensitizers. Herein, we report a new HAE mode by noncovalent cohosting of heavy atoms and photosensitizers in a double-stranded DNA (dsDNA) matrix. With dsDNA bearing several thymine (T) or cytosine (C) mismatches, heavy atoms (e.g., Hg2+ or Ag+) and dsDNA-staining dyes (photosensitizers) were spatially adjoined in close proximity, thus resulting in enhanced phosphorescence and 1O2 generation from the photosensitizers. The dsDNA-hosted HAE provides highly selective recognition for the heavy atoms, which is not applicable in either the external or the internal mode. Considering the simpleness and efficiency of the spatially adjoined HAE, as well as the functionality of DNA, the proposed HAE mode is appealing for various singlet oxygen- and phosphorescence-related applications.

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confidence: 99%

Selective Heavy Atom Effect Forming Photosensitizing Hot Spots in Double-Stranded DNA Matrix

Zhang

Liu

et al. 2021

J. Phys. Chem. Lett.

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“…In order to understand the intuitive effect of EO on the morphology of C. acnes , SEM and TEM techniques were used in this study ( Wang et al, 2021 ). As shown in Figure 2 , after 8 h of culture, the morphologies of the bacterium in the solvent control (Control) and the blank control group (CK) were complete, the surface of cells was intact, smooth, and bright, as well as the cells were full of cytoplasmic material.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of the Essential Oil From Litsea cubeba Against Cutibacterium acnes and the Investigations of Its Potential Mechanism by Gas Chromatography-Mass Spectrometry Metabolomics

et al. 2022

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Cutibacterium acnes (C. acnes) is an anaerobic Gram-positive bacterium generally considered as a human skin commensal, but is also involved in different infections, such as acne and surgical infections. Although there are a variety of treatments, the side effects and the problem of bacterial drug resistance still limit their clinical usage. In this study, we found that essential oil (EO) distilled from fresh mature Litsea cubeba possessed promising antibacterial activity against C. acnes. In order to elucidate its potential mechanism, bacteriostatic activity test, Live/Dead kit assay, scanning electron microscope (SEM), transmission electron microscope (TEM), and metabolomics were employed. In addition, the content of adenosine triphosphate (ATP) in bacterium and the activities of key enzymes involved in critical metabolic pathways were detected using a variety of biochemical assays. The results showed that EO exhibited significant antibacterial activity against C. acnes at a minimum inhibitory concentration (MIC) of 400 μg/mL and a minimum bactericidal concentration (MBC) of 800 μg/mL, and EO could destroy C. acnes morphology and inhibit its growth. Moreover, results from our study showed that EO had a significant effect on the C. acnes normal metabolism. In total, 86 metabolites were altered, and 34 metabolic pathways related to the carbohydrate metabolism, energy metabolism, amino acid metabolism, as well as cell wall and cell membrane synthesis were perturbed after EO administration. The synthesis of ATP in bacterial cells was also severely inhibited, and the activities of key enzymes of the glycolysis and Wood-Werkman cycle were significantly affected (Pyruvate Carboxylase, Malate Dehydrogenase and Pyruvate kinase activities were decreased, and Hexokinase was increased). Taken together, these results illustrated that the bacteriostatic effect of EO against C. acnes by breaking the bacterial cell morphology and perturbing cell metabolism, including inhibition of key enzyme activity and ATP synthesis. The results from our study may shed new light on the discovery of novel drugs with more robust efficacy.

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“…The result showed that the heavy-atom effect obviously increased the generation of singlet oxygen (Figure 13C). 131 However, heavy atoms often contribute to dark toxicity of PSs, and dark toxicity is one of the key problems affecting the design and performance of PS.…”

Section: Basic Principles Of Organicmentioning

confidence: 99%

“…As shown in the electrostatic potential map, the increase of the negative charge density of site A and site B accelerated the spirocyclization reaction (Figure B). The result showed that the heavy-atom effect obviously increased the generation of singlet oxygen (Figure C) . However, heavy atoms often contribute to dark toxicity of PSs, and dark toxicity is one of the key problems affecting the design and performance of PS.…”

Section: How To Design Highly Efficient Opams For Phototherapymentioning

confidence: 99%

Organic Photo-antimicrobials: Principles, Molecule Design, and Applications

et al. 2021

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The emergence of multi-drug-resistant pathogens threatens the healthcare systems world-wide. Recent advances in phototherapy (PT) approaches mediated by photo-antimicrobials (PAMs) provide new opportunities for the current serious antibiotic resistance. During the PT treatment, reactive oxygen species or heat produced by PAMs would react with the cell membrane, consequently leaking cytoplasm components and effectively eradicating different pathogens like bacteria, fungi, viruses, and even parasites. This Perspective will concentrate on the development of different organic photo-antimicrobials (OPAMs) and their application as practical therapeutic agents into therapy for local infections, wound dressings, and removal of biofilms from medical devices. We also discuss how to design highly efficient OPAMs by modifying the chemical structure or conjugating with a targeting component. Moreover, this Perspective provides a discussion of the general challenges and direction for OPAMs and what further needs to be done. It is hoped that through this overview, OPAMs can prosper and will be more widely used for microbial infections in the future, especially at a time when the global COVID-19 epidemic is getting more serious.

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Halo-fluorescein for photodynamic bacteria inactivation in extremely acidic conditions

Cited by 42 publications

References 46 publications

Selective Heavy Atom Effect Forming Photosensitizing Hot Spots in Double-Stranded DNA Matrix

Selective Heavy Atom Effect Forming Photosensitizing Hot Spots in Double-Stranded DNA Matrix

Antibacterial Activity of the Essential Oil From Litsea cubeba Against Cutibacterium acnes and the Investigations of Its Potential Mechanism by Gas Chromatography-Mass Spectrometry Metabolomics

Organic Photo-antimicrobials: Principles, Molecule Design, and Applications

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