Halo Columns: New Generation Technology for High Speed Liquid Chromatography

Abstract

Fast speed and high sample loading and the pressing demands of industries and researchers are compelling scientists and manufacturers to explore the new horizons in column technology. Recently, superficially porous silica particle columns are manufactured with some salient features such as super fast speed, sharp peaks, good sample loading, and low backpressure. The commercially available columns are Halo (Advanced Material Technology, Wilmington, DE), Express (Supelco, Bellefonte, PA), and Proshell 120 (Agilent, Santa Clara, CA). Halo columns are of C(8), C(18), RP Amide, and HILIC types with 2.7 microm over all diameters, 0.5 microm porous thick layers containing 90 A as pore diameter, and 150 m(2)/g surface area. These columns have been used for fast separation of low molecular weight compounds with some exception for large molecules such as protein, peptides, and DNA. The present article describes the importance of these state-of-the-art superficially porous silica particles based columns with special emphasis on Halo columns. The different aspects of these columns such as structures, mechanism of separations, applications, and comparison, with conventional columns have been discussed.

Superficially porous particles columns for super fast HPLC separations

“…superficially porous particles (shell particles). These porous particle columns reduced run time by 70% or more (Ali et al, 2010). These types of columns are currently manufactured by Advanced Material Technology, USA (Halo), Spelco Analytical, USA (Ascentis Express) and Phenomenex, USA (Kinetex).…”

Simultaneous Quantification of 18 Bioactive Constituents in Tripterygium wilfordii Using Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

“…An Agilent Poroshell SB C 18 chromatographic column was coupled with the triple-Q MS system. The Poroshell column was reported to provide a faster separation than conventional columns packed with 3 or 5 µm particles and also allowed a lower system pressure than columns containing 2 or 1.7 µm particles [23]. The aim of the current work was to develop an HPLC-MRM-MS method to quantify the major terpenoids in T. wilfordii and its related commercial preparations.…”

LC‐MS in analytical toxicology: some practical considerations

“…Matrix effects may be reduced when using UHPLC compared with HPLC, as interfering matrix components are more efficiently separated from compounds of interest (Chambers et al ., 2007). If UHPLC hardware is not available, superficially porous packing materials based on silica particles with non‐porous cores may offer similar gains in efficiency, but at column pressures within the range of standard HPLC pumps (Kirkland et al ., 2007; Ali et al ., 2010; Fekete et al ., 2009). Rust et al .…”