Hallmarks of Reversible Separation of Living, Unperturbed Cell Membranes into Two Liquid Phases

Rayermann, Scott; Rayermann, Glennis E.; Cornell, Caitlin E.; Merz, Alexey J.; Keller, Sarah L.

doi:10.1016/j.bpj.2017.09.029

Cited by 83 publications

(96 citation statements)

References 56 publications

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“…In addition, because lipid domains (rafts) play important roles in the association of proteins and membranes, controlling the formation of lipid domains by changing air gas concentrations may enable tuning of the amounts of membrane‐associated proteins in a lipid vesicle. Recently, Rayermann et al reported reversible membrane domain separation of yeast vacuoles by controlling ambient temperature across the transition temperature . The results of the present work suggest that air gas concentrations, especially N 2 concentration, may also be used to affect domain formation on vesicle membranes.…”

Section: Discussionsupporting

confidence: 51%

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Emerging Roles of Air Gases in Lipid Bilayers

Lee

Chiang

Liu³

et al. 2018

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Recent studies indicate that changing the physical properties of lipid bilayers may profoundly change the function of membrane proteins. Here, the effects of dissolved nitrogen and oxygen molecules on the mechanical properties and stability of lipid bilayers are investigated using differential confocal microscopy, atomic force microscopy, and molecular dynamics simulations. All experiments evidence the presence of dissolved air gas in lipid bilayers prepared without gas control. The lipid bilayers in degassed solutions are softer and less stable than those in ambient solutions. High concentrations of nitrogen increase the bending moduli and stability of the lipid bilayers and impede phase separation in ternary lipid bilayers. The effect of oxygen is less prominent. Molecular dynamics simulations indicate that higher nitrogen affinity accounts for increased rigidity. These findings have fundamental and wide implications for phenomena related to lipid bilayers and cell membranes, including the origin of life.

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Section: Discussionsupporting

confidence: 51%

“…Recently, Rayermann et al reported reversible membrane domain separation of yeast vacuoles by controlling ambient temperature across the transition temperature. [42] The results of the present work suggest that air gas concentrations, especially N 2 concentration, may also be used to affect domain formation on vesicle membranes.…”

Section: Discussionmentioning

confidence: 64%

Emerging Roles of Air Gases in Lipid Bilayers

Lee

Chiang

Liu³

et al. 2018

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“…Significant theoretical and experimental evidence supports the influence of lipid composition on membrane curvature, phase partitioning, and nanoscale domains in vesicular budding processes (53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65)(66). Many of the same observations and conclusions most likely also hold in biological processes involving membrane proteins, which complicate the picture still further.…”

Section: Discussionmentioning

confidence: 97%

Entropic forces drive clustering and spatial localization of influenza A M2 during viral budding

Madsen

Grime

Rossman

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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The influenza A matrix 2 (M2) transmembrane protein facilitates virion release from the infected host cell. In particular, M2 plays a role in the induction of membrane curvature and/or in the scission process whereby the envelope is cut upon virion release. Here we show using coarse-grained computer simulations that various M2 assembly geometries emerge due to an entropic driving force, resulting in compact clusters or linearly extended aggregates as a direct consequence of the lateral membrane stresses. Conditions under which these protein assemblies will cause the lipid membrane to curve are explored, and we predict that a critical cluster size is required for this to happen. We go on to demonstrate that under the stress conditions taking place in the cellular membrane as it undergoes large-scale membrane remodeling, the M2 protein will, in principle, be able to both contribute to curvature induction and sense curvature to line up in manifolds where local membrane line tension is high. M2 is found to exhibit linactant behavior in liquid-disordered-liquid-ordered phase-separated lipid mixtures and to be excluded from the liquid-ordered phase, in near-quantitative agreement with experimental observations. Our findings support a role for M2 in membrane remodeling during influenza viral budding both as an inducer and a sensor of membrane curvature, and they suggest a mechanism by which localization of M2 can occur as the virion assembles and releases from the host cell, independent of how the membrane curvature is produced.

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“…However, molecular dynamic simulations have revealed nanoscopic microdomains of hexagonally packed saturated lipids 30 that may correspond to the nonideal demixing revealed by FRET 31 . Recently a fungal vacuole was reported to exhibit temperaturedependent lipid phase demixing 32 but no function has yet been ascribed to these domains. The specific role of lipid asymmetry and composition, and its role in domain formation and maintenance, in the face of lipid transport, remain to be investigated for any MCS or membrane domain.…”

Section: Discussionmentioning

confidence: 99%

Contacting domains that segregate lipid from solute transporters in malaria parasites

Garten

Beck

Roth³

et al. 2019

Preprint

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While membrane contact sites (MCS) between intracellular organelles are abundant 1 , and cell-cell junctions are classically defined 2 , very little is known about the contacts between membranes that delimit extracellular junctions within cells, such as those of chloroplasts and intracellular parasites. The malaria parasite replicates within a unique organelle, the parasitophorous vacuole (PV) but the mechanism(s) are obscure by which the limiting membrane of the PV, the parasitophorous vacuolar membrane (PVM), collaborates with the parasite plasma membrane (PPM) to support the transport of proteins, lipids, nutrients, and metabolites between the cytoplasm of the parasite and the cytoplasm of the host erythrocyte (RBC). Here, we demonstrate the existence of multiple micrometer-sized regions of especially close apposition between the PVM and the PPM. To determine if these contact sites are involved in any sort of transport, we localized the PVM nutrientpermeable and protein export channel EXP2, as well as the PPM lipid transporterPfNCR1. We found that EXP2 is excluded from, but PfNCR1 is included within these regions of close apposition. Thus, these two different transport systems handling hydrophilic and hydrophobic substances, respectively, assume complementary and exclusive distributions. This new structural and molecular data assigns a functional significance to a macroscopic membrane domain.

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Hallmarks of Reversible Separation of Living, Unperturbed Cell Membranes into Two Liquid Phases

Cited by 83 publications

References 56 publications

Emerging Roles of Air Gases in Lipid Bilayers

Emerging Roles of Air Gases in Lipid Bilayers

Entropic forces drive clustering and spatial localization of influenza A M2 during viral budding

Contacting domains that segregate lipid from solute transporters in malaria parasites

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