Hairy roots of Brassica napus: I. Applied glutamine overcomes the effect of phosphinothricin treatment

Downs, C.G.; Christey, M. C.; Maddocks, D.; Seelye, J.F.; Stevenson, David G.

doi:10.1007/bf00233295

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…Root-applied Gln reversed the glufosinateinduced suppression of hairy root growth in B. napus (Downs et al, 1994). In our experiments, exogenously supplied Gln temporarily enhanced d,l-[…”

Section: Discussionsupporting

confidence: 57%

Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus

Beriault

Horsman

Devine³

1999

Plant Physiology

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“…Root-applied Gln reversed the glufosinateinduced suppression of hairy root growth in B. napus (Downs et al, 1994). In our experiments, exogenously supplied Gln temporarily enhanced d,l-[…”

Section: Discussionsupporting

confidence: 57%

Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus

Beriault

Horsman

Devine³

1999

Plant Physiology

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“…Furthermore, if peak 87 was the GDH substrate 2-oxoglutarate, the reduced Fig. These findings in gdhA tobacco, together with documented increases in whole-plant tolerance to glufosinate (Nolte et al, 2004), are consistent with suppression of glufosinate-induced phytotoxicity through the addition of glutamate in some plant species (Wendler et al, 1990;Downs et al, 1994). Plant metabolic pathway in which ammonium is assimilated and where glufosinate inhibits glutamine synthetase.…”

Section: Discussionmentioning

confidence: 57%

Glufosinate Absorption, Translocation, and Metabolic Fingerprint Effects in gdhA‐Transformed Tobacco

et al. 2017

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Tobacco (Nicotiana tabacum L.) expressing a modified Escherichia coli gdhA gene encoding NADPH‐dependent glutamate dehydrogenase (GDH) was resistant to the herbicide glufosinate. Differential plant sensitivity to glufosinate may result from changes in foliar absorption, translocation, plant metabolism, or metabolism around the blocked pathway(s). The objectives here were to determine if foliar absorption, translocation, or metabolism of glufosinate or metabolism around the blocked pathway(s) contribute to the resistance to glufosinate in gdhA‐transformed tobacco and to characterize changes in the metabolic profile in response to glufosinate as a result of transformation with gdhA. Foliar uptake and translocation of glufosinate was largely independent of the gdhA transgene or its expression. Thus, gdhA does not confer resistance to the transformed plants by altering the uptake or movement of glufosinate within tobacco plants. Using direct‐injection electrospray ionization and quadrupole time‐of‐flight mass spectrometry, the metabolic profiles of the two tobacco genotypes were different in response to glufosinate treatment, as determined by principal component analysis. The metabolic perturbation induced by glufosinate was lower in gdhA line 9 than gusA line 1, as evidenced by the reduced number of altered peaks recorded in leaves. Thus, gdhA‐transformed tobacco plants with low and high expression of GDH activity showed more stability of metabolism following the application of glufosinate than control plants. The predicted identity of ions suggested metabolic perturbations were lessened by gdhA in nitrogen assimilation, photorespiration, amino acid metabolism, nucleic acid metabolism, and cell signaling. Thus, use of the modified E. coli gdhA gene in transgenic plants can provide an additional mechanism for resistance to glufosinate.

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“…On the other hand, addition of glutamine and glutamate to PPT reduced the photosynthesis inhibition in Sinapis alba; that is, the NH 4 + accumulation cannot be the primary cause for photosynthesis inhibition by PPT [8]. Addition of glutamine also overcame a negative effect of PPT on hairy root growth in Brassica napus [16]. Analogous studies were not performed on woody species.…”

Section: Discussionmentioning

confidence: 99%

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

2015

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Transformation of plants with genes encoding a glutamine synthetase (GS), a key nitrogen metabolism enzyme, is usually used to increase productivity. However, overexpression of these genes may increase resistance to phosphinothricin (PPT) that irreversibly inhibits GS causing ammonium accumulation in plant tissues. Transgenic plants of two birch (Betula pubescens) genotypes expressing a pine cytosolic GS gene were used for studying the PPT effect on trees. Two control and 8 transgenic lines were treated with herbicide "Basta" at dose equivalent to 2.5 and 5 Lha −1 . Necrosis and abscission of leaves occurred irrespective of a transgenic status or the treatment dose. Ammonium content in leaf tissue in 3 days after the 5 Lha −1 treatment was substantially increased in all plants, 3.2-16.0 times depending on line. After the 2.5 Lha −1 treatment, ammonium content in three transgenic lines was not different from that in control variant sprayed with water. The herbicide treatment caused more prominent desiccation in the bp3f1 genotype nontransgenic plants as compared to transgenic plants, but not in the bp4a genotype. Lack of correlation between ammonium levels and survival of transgenic plants suggests that ammonium toxicity is not a main reason for the birch plant death after the PPT treatment.

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Hairy roots of Brassica napus: I. Applied glutamine overcomes the effect of phosphinothricin treatment

Cited by 12 publications

References 12 publications

Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus

Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus

Glufosinate Absorption, Translocation, and Metabolic Fingerprint Effects in gdhA‐Transformed Tobacco

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

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