Hairpin ribozyme catalysis: A surface‐enhanced Raman spectroscopy study

Percot, Aline; Lecomte, Sophie; Vergne, Jacques; Maurel, Marie‐Christine

doi:10.1002/bip.21143

Cited by 9 publications

(7 citation statements)

References 41 publications

(55 reference statements)

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“…Adenine has been the subject of numerous SERS studies since the early 2000s. However, there are some debates about how adenine adsorbs on a silver or gold surface, as well as its orientation, which are crucial parameters for the exaltation of the SERS signal [21][22][23][24][25]. There is also uncertainty about the exact assignments of some bands [16,17,[26][27][28].…”

Section: B Adenine Detectionmentioning

confidence: 99%

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Percot

Zins

Araji

et al. 2019

Life

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Space missions using probes to return dust samples are becoming more frequent. Dust collectors made of silica aerogel blocks are used to trap and bring back extraterrestrial particles for analysis. In this work, we show that it is possible to detect traces of adenine using surface-enhanced Raman spectroscopy (SERS). The method was first optimized using adenine deposition on glass slides and in glass wells. After this preliminary step, adenine solution was injected into the silica aerogel. Finally, gaseous adenine was successfully trapped in the aerogel. The presence of traces of adenine was monitored by SERS through its characteristic bands at 732, 1323, and 1458 cm−1 after the addition of the silver Creighton colloid. Such a method can be extended in the frame of Tanpopo missions for studying the interplanetary transfer of prebiotic organic compounds of biological interest.

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Section: B Adenine Detectionmentioning

confidence: 99%

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Percot

Zins

Araji

et al. 2019

Life

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“…Surface-enhanced Raman scattering (SERS) was discovered in 1974 and has since been an important analytical tool in several applications, including biological sensing, [1,2] trace analysis, [3] and catalysis. [4] There are primarily two mechanisms that are responsible for the SERS effect: The first is the electromagnetic enhancement (EE) mechanism that occurs in probe molecules that are deposited onto roughened noble-metal surfaces (e.g., silver or gold). [5] In this case, the incident light is used to excite the surface plasmons of a metallic substrate, which creates a strong local electric field that is capable of interacting with the molecule and enhancing the Raman signal.…”

Section: Introductionmentioning

confidence: 99%

Surface‐Enhanced Raman Scattering (SERS) of Nitrothiophenol Isomers Chemisorbed on TiO₂

Teguh

Liu

Xing

et al. 2012

Chemistry An Asian Journal

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Surface-enhanced Raman scattering (SERS) spectroscopy and density functional theory (DFT) calculations were used to investigate the nature of the charge-transfer (CT) process between nitrothiophenol (NTP) isomers and the n-type semiconductor, TiO(2). The Raman signals of p-NTP and m-NTP that were chemisorbed onto TiO(2) were significantly enhanced with respect to their corresponding neat compounds. In particular, an enhancement factor (EF) of 10(2)-10(3) was observed for both p-NTP and m-NTP, with m-NTP displaying a larger EF compared to p-NTP. The Raman signal of o-NTP on TiO(2) was not detectable, owing to interference from fluorescence emissions. A molecule-to-TiO(2) charge-transfer mechanism was responsible for the enhanced Raman signals observed in p-NTP and m-NTP. This transfer was due to a strong coupling between the adsorbate and the metal oxide, which led to an optically driven CT transition from the HOMO of NTP into the conduction band of TiO(2). Based on the mesomeric effect, the NO(2) group para to the thiol had a stronger electron-withdrawing ability than the NO(2) group at the meta position. A less-efficient CT transition from p-NTP to TiO(2) in the surface complex resulted in a weaker Raman-signal enhancement for p-NTP compared to m-NTP. The DFT calculation determined that the HOMO and the LUMO of NTP bound to TiO(2) were located entirely on the adsorbate and the semiconductor, respectively, thereby supporting the experimental findings that a molecule-to-TiO(2) mechanism was the driving force behind the observed SERS effect.

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“…Presence of CO bonds and aromatic cycles promotes electronic interaction of AMC (Fig. b) with Ag° colloids . Consequently modes involving these chemical bonds were enhanced.…”

Section: Resultsmentioning

confidence: 97%

“…1b) with Ag°colloids. [41][42][43] Consequently modes involving these chemical bonds were enhanced. Particularly in the SERS spectrum, the band at 1586 cm À1 was identified to be the most intense and was thus selected as a standard band for quantitation studies.…”

Section: Resultsmentioning

confidence: 99%

Amino‐methyl coumarin as a potential SERS@Ag probe for the evaluation of protease activity and inhibition

Soualmia

Touhar

Guo

et al. 2016

J Raman Spectroscopy

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Proteases are found deregulated in many diseases including cancer and neurodegenerative diseases. They thus represent good therapeutical targets for the development of inhibitors mainly small organic molecules. Peptide substrates containing fluorogenic groups constitute central tools for the monitoring of protease activities and inhibitor screening platforms. Amino‐methyl coumarin (AMC) is a well‐known fluorogenic group that functionalized a huge number of peptide substrates used for kinetics in vitro but also in vivo. However, either autofluorescence or quenching of the AMC fluorescence could compromise selection and accurate evaluation of these inhibitors. It is thus needed to explore alternative spectroscopic tools to unravel these limitations. Here, we investigate whether AMC could constitute a valuable Surface Enhanced Raman Spectroscopy probe in the presence of Creighton' silver colloids under 532‐nm excitation to monitor protease activity and to evaluate inhibitors. The kallikrein‐related peptidase 8 was used as model of proteolytic enzyme. Band‐Target Entropy Minimization analysis was successfully used to validate the present Surface Enhanced Raman Spectroscopy approach. Copyright © 2016 John Wiley & Sons, Ltd.

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Hairpin ribozyme catalysis: A surface‐enhanced Raman spectroscopy study

Cited by 9 publications

References 41 publications

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Surface‐Enhanced Raman Scattering (SERS) of Nitrothiophenol Isomers Chemisorbed on TiO₂

Amino‐methyl coumarin as a potential SERS@Ag probe for the evaluation of protease activity and inhibition

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Hairpin ribozyme catalysis: A surface‐enhanced Raman spectroscopy study

Cited by 9 publications

References 41 publications

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Detection of Biological Bricks in Space. The Case of Adenine in Silica Aerogel

Surface‐Enhanced Raman Scattering (SERS) of Nitrothiophenol Isomers Chemisorbed on TiO2

Amino‐methyl coumarin as a potential SERS@Ag probe for the evaluation of protease activity and inhibition

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Surface‐Enhanced Raman Scattering (SERS) of Nitrothiophenol Isomers Chemisorbed on TiO₂