Hairpin/DNA ring ternary probes for highly sensitive detection and selective discrimination of microRNA among family members

Liu, Xiaoxia; Zou, Mengqi; Li, Daxiu; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.aca.2019.05.027

Cited by 17 publications

(10 citation statements)

References 38 publications

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“…For example, Liu et al demonstrated that a circular DNA could be formed in the presence of the ligase and the DNA (Fig. 1 B) [ 22 ]. Then, with the addition of the prime DNA, RCA process was executed to generate numerous repeated DNA sequences.…”

Section: Cyclic Signal Amplification Strategiesmentioning

confidence: 99%

“…1 A Schematic illustration of circDNA as a template for RCA [ 21 ]. B Schematic diagram of sensitive detection of miRNA based on RCA (Linear DNA as a template) combined with DNAzyme [ 22 ] …”

Section: Cyclic Signal Amplification Strategiesmentioning

confidence: 99%

“…For example, Liu et al . designed a hairpin/DNA ring ternary probe for highly selective and sensitive detection of microRNA (miRNA) by combining RCA with metal ion dependent DNA enzyme (DNAzyme) for signal amplification [ 22 ] (Fig. 1 B).…”

Section: Cyclic Signal Amplification Strategiesmentioning

confidence: 99%

See 2 more Smart Citations

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Fan

Chen

et al. 2021

J Nanobiotechnol

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The cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

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Section: Cyclic Signal Amplification Strategiesmentioning

confidence: 99%

Section: Cyclic Signal Amplification Strategiesmentioning

confidence: 99%

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Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Fan

Chen

et al. 2021

J Nanobiotechnol

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“…Among them, the electrochemical aptasensor is the most extensively used because of its operational feasibility and sensitivity [15][16]. In addition, different signal amplification techniques such as rolling circle amplification (RCA) [17][18], hybridization chain reaction (HCR) [19][20] and catalytic hairpin assembly (CHA) [21][22] are also incorporated with these detection methods to further improve detection sensitivity. Interestingly, CHA has higher catalytic efficiency, lower background signal, simpler and more stable reaction system [23][24].…”

Section: Introductionmentioning

confidence: 99%

Enzyme‐free Recycling Amplification‐based Sensitive Electrochemical Thrombin Aptasensor

Zhang¹,

Liao²,

Wang³

et al. 2021

Electroanalysis

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In this work, an enzyme‐free recycling amplification‐based sensitive electrochemical thrombin aptasensor using catalytic hairpin assembly (CHA) and bisferrocene was proposed for electrochemical beacon. Thrombin triggered the CHA reaction that in turn formed a number of double‐stranded complexes,which are fixed on the surface of the gold electrode through potential‐assisted Au−S deposition, and a large number of labeled bisferrocene are placed close to the gold electrode to generate electrochemical signal. The linear range of the electrochemical sensor was 0.25 pM‐2.5 nM for thrombin with detection limit of 0.18 pM. This sensor can be employed to monitor the thrombin in human serum samples.

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“…For instance, with the design of a new hairpin/deoxyribonucleic acid (DNA) ring ternary probe in RCA, the target miRNA can bind the probes through toehold-mediated strand displacement to form ternary structures and generate many repeated metal ion-dependent deoxyribozyme (DNAzyme) sequences. Finally, fluorescently quenched hairpin signal probes can be cyclically cleaved by the DNAzyme sequences, which drastically enhances the fluorescence recovery of the target miRNA ( Liu et al, 2019 ). Tian et al (2019) rationally combined RCA with efficient loop-mediated isothermal amplification (LAMP), which directly templates the ligation of a padlock probe to trigger the RCA reaction.…”

Section: Introductionmentioning

confidence: 99%

Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b

Long

Wang

et al. 2021

Front. Plant Sci.

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Microribonucleic acids (miRNAs) play significant roles in the regulation of biological processes and in responses to biotic or abiotic environmental stresses. Therefore, it is necessary to quantitatively detect miRNAs to understand these complicated biological regulation mechanisms. This study established an ultrasensitive and highly specific method for the quantitative detection of miRNAs using simple operations on the ground of the ligation reaction of ribonucleotide-modified deoxyribonucleic acid (DNA) probes. This method avoids the complex design of conventional reverse transcription. In the developed assay, the target miRNA miR156b was able to directly hybridize the two ribonucleotide-modified DNA probes, and amplification with universal primers was achieved following the ligation reaction. As a result, the target miRNA could be sensitively measured even at a detection limit as low as 0.0001 amol, and differences of only a single base could be detected between miR156 family members. Moreover, the proposed quantitative method demonstrated satisfactory results for overexpression-based genetically modified (GM) soybean. Ligation-based quantitative polymerase chain reaction (PCR) therefore has potential in investigating the biological functions of miRNAs, as well as in supervising activities regarding GM products or organisms.

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Hairpin/DNA ring ternary probes for highly sensitive detection and selective discrimination of microRNA among family members

Cited by 17 publications

References 38 publications

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

Enzyme‐free Recycling Amplification‐based Sensitive Electrochemical Thrombin Aptasensor

Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b

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