Hairpin DNA assisted dual-ratiometric electrochemical aptasensor with high reliability and anti-interference ability for simultaneous detection of aflatoxin B1 and ochratoxin A

Zhu, Chengxi; Liu, Dong; Li, Yuye; Ma, Shuai; Wang, Meng; You, Tianyan

doi:10.1016/j.bios.2020.112654

Cited by 118 publications

(46 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After the aptamer fixed on the electrode surface combines with the target, the concentration of the target is detected by collecting the changes of electrochemical signals such as voltage, current, conductivity and impedance [ 121 ]. Common electrochemical methods include sandwich method, competition method and electrochemical impedance spectroscopy method [ 122 , 123 ]. In addition, electrochemical sensors can also be combined with electrochemical signal amplification strategy to achieve the purpose of amplifying the signal.…”

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

View full text Add to dashboard Cite

Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

show abstract

Section: Aptasensors For Heavy Metals Ion Detectionmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Fig. 3 A shows a hairpin DNA-based ratiometric electrochemical aptasensor for simultaneous detection of AFB1 and OTA [ 120 ]. Ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2) served as binding probes and current signal indicators.…”

Section: Rapid Detection Strategiesmentioning

confidence: 99%

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Yang

Wang

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional detection methods can no longer meet the needs of massive, real-time, simple, and fast mycotoxin monitoring. Rapid detection methods based on advanced material and sensor technology are the future trend. In this review, we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods, especially for simultaneous multiplex mycotoxin determination. Immunoassays, biosensors, and the prominent roles of nanomaterials are introduced. The principles of different types of recognition and signal transduction are explained, and the merits and pitfalls of these methods are compared. Furthermore, limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.

show abstract

“…The selected redox compound should also show high stability and the ability to bind to nanoparticles or to form conjugates with the applicable receptor molecule without affecting its recognition functionality [ 66 , 71 ]. From among the aptasensors proposed for simultaneous multiple analytes detection, two types of sensors have most often been considered: Structure-switching sensors [ 72 ], in which the redox-tag labelled aptamer alters the conformation upon binding a target molecule, resulting in changes in the rate of electron transfer between the redox tag and electrode surface [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 ], and Sensors based on bioconjugates containing nanomaterials, such as gold nanoparticles (AuNPs), gold nanorods (AuNRs), or nano zirconium-metal organic framework (NMOF), modified further with aptamer and redox tag molecules [ 70 , 84 , 85 , 86 ]. …”

Section: Redox-probe Based Electrochemical Aptasensors For Simultaneous Detection Of Multiple Analytesmentioning

confidence: 99%

“…Structure-switching sensors [ 72 ], in which the redox-tag labelled aptamer alters the conformation upon binding a target molecule, resulting in changes in the rate of electron transfer between the redox tag and electrode surface [ 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 ], and…”

Section: Redox-probe Based Electrochemical Aptasensors For Simultaneous Detection Of Multiple Analytesmentioning

confidence: 99%

“…Multiplex electrochemical aptasensors have recently been applied for the detection of common toxins in food samples. A novel ratiometric electrochemical aptasensor has been reported for simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) [ 82 ]. In the construction of the sensing layer in this multiplex sensor ( Figure 3 , reprinted from ref.…”

Section: Redox-probe Based Electrochemical Aptasensors For Simultaneous Detection Of Multiple Analytesmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Design Strategies of Multiplex Electrochemical Aptasensors

Grabowska

Hepel

Kurzątkowska-Adaszyńska

2021

Sensors

View full text Add to dashboard Cite

In recent years, the need for simple, fast, and economical detection of food and environmental contaminants, and the necessity to monitor biomarkers of different diseases have considerably accelerated the development of biosensor technology. However, designing biosensors capable of simultaneous determination of two or more analytes in a single measurement, for example on a single working electrode in single solution, is still a great challenge. On the other hand, such analysis offers many advantages compared to single analyte tests, such as cost per test, labor, throughput, and convenience. Because of the high sensitivity and scalability of the electrochemical detection systems on the one hand and the specificity of aptamers on the other, the electrochemical aptasensors are considered to be highly effective devices for simultaneous detection of multiple-target analytes. In this review, we describe and evaluate multi-label approaches based on (1) metal quantum dots and metal ions, (2) redox labels, and (3) enzyme labels. We focus on recently developed strategies for multiplex sensing using electrochemical aptasensors. Furthermore, we emphasize the use of different nanomaterials in the construction of these aptasensors. Based on examples from the existing literature, we highlight recent applications of multiplexed detection platforms in clinical diagnostics, food control, and environmental monitoring. Finally, we discuss the advantages and disadvantages of the aptasensors developed so far, and debate possible challenges and prospects.

show abstract

Hairpin DNA assisted dual-ratiometric electrochemical aptasensor with high reliability and anti-interference ability for simultaneous detection of aflatoxin B1 and ochratoxin A

Cited by 118 publications

References 38 publications

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Advances in Design Strategies of Multiplex Electrochemical Aptasensors

Contact Info

Product

Resources

About