Hair Tonic of Broccoli (Brassica Oleracea Var. Italica) Extract: Formulation, Physical Characteristic, Stability and Hair Growth Activity Test

Abstract

Objective: Hair loss is a common scalp concern for both men and women. Broccoli (Brassica oleracea var. Italica) is a vegetable plant in the cabbage family or brassicaceae that has antioxidant activity and levels of flavonoids that can encourage hair growth. Hair tonic is one of cosmetic products that contain ingredients to nourish hair. The purpose of this study was to investigate the physical stability and activity of hair development in hair tonic preparations using broccoli stalks extracted at different concentrations of 30%, 35%, and 40%, followed by an examination of the hair growth activity test in an animal model. Methods: This study hair tonic was prepared with Broccoli (Brassica oleracea var. Italica), the sample used the broccoli stalks, which was taken by purposive sampling. Evaluation of the preparation includes physical characteristic observation, pH examination, homogeneity of the preparation, irritation test, and stability test of the preparation using a cycling test, and check the activity test of hair growth in rats’ skin. Results: The results showed that the hair tonic preparation containing broccoli stalks extracts was stable at a temperature (4 °C±40 °C) where the odor, color, shape, homogeneity, and pH remained constant. In statistical testing using ANOVA analysis, each group of the hair tonic formula of broccoli stalks extract showed hair growth activity in male white rats. The hair tonic formulation containing 40% broccoli stalks extract had better hair growth activity compared to the 30% and 35% formula groups. The hair tonic prepared from broccoli extract is confirmed safe for use after successfully passing the skin irritation test. Conclusion: The hair tonic of broccoli extracts had met the standard of physical characteristics during storage time and it has a good activity test on hair growth in male white rats. This study provides a novel formulation in hair growth.