Hair removal and bioavailability of chemicals: Effect of physicochemical properties of drugs and surfactants on skin permeation ex vivo

Pany, Astrid; Klang, Victoria; Peinhopf, Caroline; Zecevic, Angela; Ruthofer, Johanna; Valenta, Claudia

doi:10.1016/j.ijpharm.2019.118477

Cited by 7 publications

(5 citation statements)

References 30 publications

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“… 31 A subsequent study by the same group using model drugs with various physicochemical properties showed all hair removal methods enhanced skin permeation to varying degrees, with no discernable pattern indicating that any technique was superior to another. 32 However, depilation cream did not enhance the dermal flux of fluconazole (LogP of 0.45 ± 0.84) and thus may not improve the partitioning of hydrophilic NM into the skin. The electric clipping method used in the current studies is distinct from dry shaving as it removes most of the hair shaft without exposing the skin surface directly to the razors.…”

Section: Discussionmentioning

confidence: 93%

“…Dry shaving, electric epilation, and waxing resulted in a moderate reduction in barrier function, whereas depilation cream and wet shaving did not 31 . A subsequent study by the same group using model drugs with various physicochemical properties showed all hair removal methods enhanced skin permeation to varying degrees, with no discernable pattern indicating that any technique was superior to another 32 . However, depilation cream did not enhance the dermal flux of fluconazole (LogP of 0.45 ± 0.84) and thus may not improve the partitioning of hydrophilic NM into the skin.…”

Section: Discussionmentioning

confidence: 99%

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Depilatory double‐disc mouse model for evaluation of vesicant dermal injury pharmacotherapy countermeasures

Roldan

Laskin

et al. 2023

Anim Models and Exp Med

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Background Sulfur mustard (SM) is a chemical warfare vesicant that severely injures exposed eyes, lungs, and skin. Mechlorethamine hydrochloride (NM) is widely used as an SM surrogate. This study aimed to develop a depilatory double‐disc (DDD) NM skin burn model for investigating vesicant pharmacotherapy countermeasures. Methods Hair removal method (clipping only versus clipping followed by a depilatory), the effect of acetone in the vesicant administration vehicle, NM dose (0.5–20 μmol), vehicle volume (5–20 μl), and time course (0.5–21 days) were investigated using male and female CD‐1 mice. Edema, an indicator of burn response, was assessed by biopsy skin weight. The ideal NM dose to induce partial‐thickness burns was assessed by edema and histopathologic evaluation. The optimized DDD model was validated using an established reagent, NDH‐4338, a cyclooxygenase, inducible nitric oxide synthase, and acetylcholinesterase inhibitor prodrug. Results Clipping/depilatory resulted in a 5‐fold higher skin edematous response and was highly reproducible (18‐fold lower %CV) compared to clipping alone. Acetone did not affect edema formation. Peak edema occurred 24–48 h after NM administration using optimized dosing methods and volume. Ideal partial‐thickness burns were achieved with 5 μmol of NM and responded to treatment with NDH‐4338. No differences in burn edematous responses were observed between males and females. Conclusion A highly reproducible and sensitive partial‐thickness skin burn model was developed for assessing vesicant pharmacotherapy countermeasures. This model provides clinically relevant wound severity and eliminates the need for organic solvents that induce changes to the skin barrier function.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Depilatory double‐disc mouse model for evaluation of vesicant dermal injury pharmacotherapy countermeasures

Roldan

Laskin

et al. 2023

Anim Models and Exp Med

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“…In addition, potential changes in SC thickness due to physical removal of corneocytes have to be taken into account in data evaluation. 97,98 In a recent study, the effect of iontophoresis and sonophoresis on barrier function alone and in combination with a chemical enhancer was shown for a model permeant using mouse skin and surface-enhanced Raman spectroscopy 99 ; the best option for oxaprozin delivery was found to be sonophoresis combined with azone as enhancer.…”

Section: Skin Composition Studiesmentioning

confidence: 99%

“…Apart from the effect of chemical stressors, the impact of physical procedures such as tape stripping, microneedling or hair removal, is possible, but less promising since not all physical stressors evoke significant changes in the chemical composition of the skin. In addition, potential changes in SC thickness due to physical removal of corneocytes have to be taken into account in data evaluation 97,98 . In a recent study, the effect of iontophoresis and sonophoresis on barrier function alone and in combination with a chemical enhancer was shown for a model permeant using mouse skin and surface‐enhanced Raman spectroscopy 99 ; the best option for oxaprozin delivery was found to be sonophoresis combined with azone as enhancer.…”

Section: Utilization Of Raman Spectroscopy In Skin Researchmentioning

confidence: 99%

Novel aspects of Raman spectroscopy in skin research

Lunter

Klang

Kocsis

et al. 2022

Experimental Dermatology

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The analytical technology of Raman spectroscopy has an almost 100‐year history. During this period, many modifications and developments happened in the method like discovery of laser, improvements in optical elements and sensitivity of spectrometer and also more advanced light detection systems. Many types of the innovative techniques appeared (e.g. Transmittance Raman spectroscopy, Coherent Raman Scattering microscopy, Surface‐Enhanced Raman scattering and Confocal Raman spectroscopy/microscopy). This review article gives a short description about these different Raman techniques and their possible applications. Then, a short statistical part is coming about the appearance of Raman spectroscopy in the scientific literature from the beginnings to these days. The third part of the paper shows the main application options of the technique (especially confocal Raman spectroscopy) in skin research, including skin composition analysis, drug penetration monitoring and analysis, diagnostic utilizations in dermatology and cosmeto‐scientific applications. At the end, the possible role of artificial intelligence in Raman data analysis and the regulatory aspect of these techniques in dermatology are briefly summarized. For the future of Raman Spectroscopy, increasing clinical relevance and in vivo applications can be predicted with spreading of non‐destructive methods and appearance with the most advanced instruments with rapid analysis time.

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“…In this area confocal Raman spectroscopy (CRS) has emerged as a promising non-invasive tool in skin research and caught increasing attention for skin characterizations such as skin penetration and permeation of topically applied materials [24,25]. CRS is also an efficient and label-free method and has been increasingly used to study the macroscopic alterations of skin properties with humidity changes, age difference and hair removal comparisons [26][27][28]. Detailed information about biochemical molecules can be obtained from CRS, including the chemical structure, phase and molecular interactions.…”

Section: Introductionmentioning

confidence: 99%

Systematic Investigation of the Effect of Non-Ionic Emulsifiers on Skin by Confocal Raman Spectroscopy—A Comprehensive Lipid Analysis

Liu

Lunter

2020

Pharmaceutics

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Non-ionic emulsifiers are commonly found in existing pharmaceutical and cosmetic formulations and have been widely employed to enhance the penetration and permeation of active ingredients into the skin. With the potential of disrupting skin barrier function and increasing fluidity of stratum corneum (SC) lipids, we herein examined the effects of two kinds of non-ionic emulsifiers on intercellular lipids of skin, using confocal Raman spectroscopy (CRS) with lipid signals on skin CRS spectrum. Non-ionic emulsifiers of polyethylene glycol alkyl ethers and sorbitan fatty acid esters were studied to obtain a deep understanding of the mechanism between non-ionic emulsifiers and SC lipids. Emulsifier solutions and dispersions were prepared and applied onto excised porcine skin. Water and sodium laureth sulfate solution (SLS) served as controls. SC lipid signals were analysed by CRS regarding lipid content, conformation and lateral packing order. Polyethylene glycol (PEG) sorbitan esters revealed no alteration of intercellular lipid properties while PEG-20 ethers appeared to have the most significant effects on reducing lipid content and interrupting lipid organization. In general, the polyoxyethylene chain and alkyl chain of PEG derivative emulsifiers might indicate their ability of interaction with SC components. HLB values remained critical for complete explanation of emulsifier effects on skin lipids. With this study, it is possible to characterize the molecular effects of non-ionic emulsifiers on skin lipids and further deepen the understanding of enhancing substance penetration with reduced skin barrier properties and increased lipid fluidity.

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Hair removal and bioavailability of chemicals: Effect of physicochemical properties of drugs and surfactants on skin permeation ex vivo

Cited by 7 publications

References 30 publications

Depilatory double‐disc mouse model for evaluation of vesicant dermal injury pharmacotherapy countermeasures

Depilatory double‐disc mouse model for evaluation of vesicant dermal injury pharmacotherapy countermeasures

Novel aspects of Raman spectroscopy in skin research

Systematic Investigation of the Effect of Non-Ionic Emulsifiers on Skin by Confocal Raman Spectroscopy—A Comprehensive Lipid Analysis

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