Hair Growth Effect of DN106212 in C57BL/6 Mouse and Its Network Pharmacological Mechanism of Action

Abstract

Centipeda minima (CMX) has been widely investigated using network pharmacology and clinical studies for its effects on hair growth via the JAK/STAT signaling pathway. Human hair follicle papilla cells exhibit hair regrowth through the expression of Wnt signaling-related proteins. However, the mechanism of action of CMX in animals has not been elucidated fully. This study examined the effect of induced hair loss and its side-effects on the skin, and observed the mechanism of action of an alcoholic extract of CMX (DN106212) on C57BL/6 mice. Our results showed that DN106212 was more effective in promoting hair growth than dimethyl sulfoxide in the negative control and tofacitinib (TF) in the positive control when mice were treated with DN106212 for 16 days. We confirmed that DN106212 promotes the formation of mature hair follicles through hematoxylin and eosin staining. We also found that the expression of vascular endothelial growth factor (Vegfa), insulin-like growth factor 1 (Igf1), and transforming growth factor beta 1 (Tgfb1) is related to hair growth using PCR. DN106212-treated mice had significantly higher expression of Vegfa and Igf1 than TF-treated ones, and inhibiting the expression of Tgfb1 had similar effects as TF treatment. In conclusion, we propose that DN106212 increases the expression of hair growth factors, promotes the development of hair follicles, and promotes hair growth. Although additional experiments are needed, DN106212 may serve as an experimental basis for research on natural hair growth-promoting agents.

Gynostemma pentaphyllum Hydrodistillate and Its Major Component Damulin B Promote Hair Growth-Inducing Properties In Vivo and In Vitro via the Wnt/β-Catenin Pathway in Dermal Papilla Cells

“…A hair growth score was calculated according to a previous report [35] in the following way. The hair growth phase was partitioned into three distinct areas: a pink region denoted as A, representing minimal hair growth; a grey section marked as B, indicating slight hair growth; and a black area labeled as C, signifying nearly complete hair growth.…”

“…Figure 1B illustrates one of the images used for quantifying hair growth on the dorsal skin of mice, with distinct colors denoting various phases of hair growth. A hair growth score was calculated according to the method described in a previous report [35], and pink, grey, and black areas were quantified using Image J software (v1.54i) to determine the hair growth score, revealing a 2.8-2.9-fold increase in hair growth for both the minoxidil and GPHD groups. Skin tissue was collected on day 15 after shaving and was processed using H&E staining for histological analysis.…”

Tổng Quan Về Sinh Học Của Tóc, Mô Hình Nghiên Cứu Và Đánh Giá Khả Năng Mọc Lại Tóc Trên in-Vitro Và in-Vivo

“…Thêm vào đó, sự chuyển đổi của testosteron thành 5-alpha-dihydrotestosteron thông qua enzym 5 alpha-reductase có thể làm giảm sự phát triển của lông. Ngược lại, khi thiếu enzyme 5 alpha-reductase, có thể dẫn đến việc lông mọc dày và khỏe mạnh [11], [12].…”

“…-Chuột đen C57BL/6 thường được sử dụng để tạo ra các mô hình không sắc tố da và có thể quan sát sớm các giai đoạn tái phát triển anagen mới của tóc [12], [14].…”