“…However the possibility of a pluripotent stem cell giving rise to partially committed progenitor cells restricted along a specific cell lineage cannot be excluded. 31 Similar observations have recently been made in a hair reconstitution assay using marked mouse keratinocytes. 32 Loss of RRV-mediated transgene expression in vivo has been reported in several systems including epidermal keratinocytes and attributed to inactivation of the viral promoter.…”
Section: Figure 5 Persistent Expression Of Rrv-lzrn Transduced Keratisupporting
confidence: 76%
“…Unlike interfollicular epidermis which contains a single type of differentiated keratinocyte, hair follicles are composed of a minimum of seven distinct cell types. 31 It is not known if these different cell types originate from single or multiple pro-genitor cells, although the hair follicles with singly marked inner or outer root sheath occasionally seen in this study would argue for multiple progenitor cells. However the possibility of a pluripotent stem cell giving rise to partially committed progenitor cells restricted along a specific cell lineage cannot be excluded.…”
Section: Figure 5 Persistent Expression Of Rrv-lzrn Transduced Keratimentioning
confidence: 69%
“…SENCAR and CD-1 mice have occasionally been used for investigations in skin biology and kinetics of hair cycles is known. 30,31 At this age, the dorsal skin of mice is in the resting phase of the hair growth cycle. The clipped, depilated backs of mice were dermabraded using a felt wheel (5/8Љ diameter, 1/8Љ thickness) connected to a rotary motor tool (Craftsman, Sears, USA) spinning at approximately 15 000 r.p.m.…”
Section: Animals and In Vivo Transductionmentioning
“…However the possibility of a pluripotent stem cell giving rise to partially committed progenitor cells restricted along a specific cell lineage cannot be excluded. 31 Similar observations have recently been made in a hair reconstitution assay using marked mouse keratinocytes. 32 Loss of RRV-mediated transgene expression in vivo has been reported in several systems including epidermal keratinocytes and attributed to inactivation of the viral promoter.…”
Section: Figure 5 Persistent Expression Of Rrv-lzrn Transduced Keratisupporting
confidence: 76%
“…Unlike interfollicular epidermis which contains a single type of differentiated keratinocyte, hair follicles are composed of a minimum of seven distinct cell types. 31 It is not known if these different cell types originate from single or multiple pro-genitor cells, although the hair follicles with singly marked inner or outer root sheath occasionally seen in this study would argue for multiple progenitor cells. However the possibility of a pluripotent stem cell giving rise to partially committed progenitor cells restricted along a specific cell lineage cannot be excluded.…”
Section: Figure 5 Persistent Expression Of Rrv-lzrn Transduced Keratimentioning
confidence: 69%
“…SENCAR and CD-1 mice have occasionally been used for investigations in skin biology and kinetics of hair cycles is known. 30,31 At this age, the dorsal skin of mice is in the resting phase of the hair growth cycle. The clipped, depilated backs of mice were dermabraded using a felt wheel (5/8Љ diameter, 1/8Љ thickness) connected to a rotary motor tool (Craftsman, Sears, USA) spinning at approximately 15 000 r.p.m.…”
Section: Animals and In Vivo Transductionmentioning
“…It is a thick skin analogous to human palmar and plantar epidermis. Further footpad epidermis allowed examination of EpiSCs from interfollicular epidermis, thereby excluding the separate population of stem cells residing in the bulge region of hair follicles (Lavker et al, 1993;Ito et al, 2005). Additionally, it ensured that tissue samples were taken from the exact same area of each animal.…”
SummaryThe epidermis of the skin, acting as the primary physical barrier between self and environment, is a dynamic tissue whose maintenance is critical to the survival of an organism. Like most other tissues and organs, the epidermis is maintained and repaired by a population of resident somatic stem cells. The epidermal stem cells reside in the proliferative basal cell layer and are believed to persist for the lifetime of an individual. Acting through intermediaries known as transit amplifying cells, epidermal stem cells ensure that the enormous numbers of keratinocytes required for epidermal homeostasis to be maintained are generated. This continual demand for new cell production must be met over the entire lifetime of an individual. Breakdown of the epidermal barrier would have catastrophic consequences. This leads us to question whether or not epidermal stem cells represent a unique population of cells which, by necessity, might be resistant to cellular aging. We hypothesized that the full physiologic functional capacity of epidermal stem cells is maintained over an entire lifetime. Using murine skin epidermis as our model system, we compared several properties of young and old adult epidermal stem cells. We found that, over an average mouse's lifetime, there was no measurable loss in the physiologic functional capacity of epidermal stem cells, leading us to conclude that murine epidermal stem cells resist cellular aging.
“…Taylor et al (2000) have reported that the bulge region of hair follicle, which is the part of the outer root sheath, harbours the stem cells that can form not only the follicle but also the epidermis. These stem cells are thought to be the major targets of tumour initiating agents by chemical carcinogenesis (Morris et al, 1986;Lavker et al, 1993). Tumour promotion involves clonal expansion of initiated cells resulting in the formation of increased number of papillomas and few carcinomas.…”
Enhancing factor (EF), a growth factor modulator, is the mouse homologue of human secretory group II phospholipase A 2 . EF exhibits growth-promoting activity in vitro, in the presence of epidermal growth factor, and also brings about phenotypic transformation of normal cells. In order to ascertain the role of EF in vivo, a human keratin-14 promoter was used to drive the expression of EF ectopically to squamous epithelial cells. The founder mouse and its progeny showed abnormal whiskers and a scaly, beaded tail. In these mice, keratinization pattern of the epidermis was disturbed and parakeratosis and acanthosis were noted. The transgenic mice, TgK14-EF, expressed EF in the suprabasal layers of tail epidermis as well as in the epithelial cells of hair follicle and sebaceous glands of skin. Expression of EF along with hyperplasia was also observed in other squamous epithelia such as buccal mucosa, tongue and oesophagus. TgK14-EF mice homozygous for the transgene showed delayed and scanty hair growth although the mice were healthy and fertile. The hemizygous TgK14-EF mice were sensitive to a twostage chemical carcinogenesis and developed a higher number of papillomas than their normal littermates over the course of the experiment. The conversion rate of papilloma to carcinoma was two fold higher in the transgenic mice.
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