Hair follicle dermal stem cells and skin‐derived precursor cells: Exciting tools for endogenous and exogenous therapies

Agabalyan, Natacha A.; Rosin, Nicole L.; Rahmani, Waleed; Biernaskie, Jeff

doi:10.1111/exd.13359

Cited by 29 publications

(21 citation statements)

References 28 publications

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“…These cells therefore not only represent promising candidates for future autologous therapy, but also for allogeneic cell-based off-the-shelf applications. Their translational potential is being investigated in preclinical animal models of spinal cord injury [7,24], bone repair [8] and wound healing [25][26][27][28]. In many of these degenerative diseases, however, a pronounced inflammatory microenvironment is present [29][30][31] that might increase the immunogenicity of SKP, facilitate their rejection and thus hamper their future therapeutic application.…”

Section: Introductionmentioning

confidence: 99%

Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Kock

Rodrigues

Branson

et al. 2020

Cells

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Human skin-derived precursors (SKP) represent a group of somatic stem/precursor cells that reside in dermal skin throughout life that harbor clinical potential. SKP have a high self-renewal capacity, the ability to differentiate into multiple cell types and low immunogenicity, rendering them key candidates for allogeneic cell-based, off-the-shelf therapy. However, potential clinical application of allogeneic SKP requires that these cells retain their therapeutic properties under all circumstances and, in particular, in the presence of an inflammation state. Therefore, in this study, we investigated the impact of pro-inflammatory stimulation on the secretome and immunosuppressive properties of SKP. We demonstrated that pro-inflammatory stimulation of SKP significantly changes their expression and the secretion profile of chemo/cytokines and growth factors. Most importantly, we observed that pro-inflammatory stimulated SKP were still able to suppress the graft-versus-host response when cotransplanted with human PBMC in severe-combined immune deficient (SCID) mice, albeit to a much lesser extent than unstimulated SKP. Altogether, this study demonstrates that an inflammatory microenvironment has a significant impact on the immunological properties of SKP. These alterations need to be taken into account when developing allogeneic SKP-based therapies.

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Section: Introductionmentioning

confidence: 99%

Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Kock

Rodrigues

Branson

et al. 2020

Cells

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“…Here, it is thought they either undergo apoptosis or incorporate into the hair follicle dermal stem cell (hfDSC) niche, while similarly in humans, apoptosis has been observed in the DS, reinforcing this suggestion that DP cells apoptose once outside the DP . Transitioning from telogen‐anagen, there is a small amount of cell division within the DP itself, however, the majority of the increase in cell number is due to an influx of cells from the DS, populated by proliferation of hfDSCs in late telogen and early anagen . Thus, if miniaturisation occurs during one cyclic transition, either the efflux from the DP is too great or the influx from the DS is too little (Figure ).…”

Section: Hair Today Gone Tomorrowmentioning

confidence: 71%

“…[20] Transitioning from telogen-anagen, there is a small amount of cell division within the DP itself, [20] however, the majority of the increase in cell number is due to an influx of cells from the DS, populated by proliferation of hfDSCs in late telogen and early anagen. [19,21] Thus, if miniaturisation occurs during one cyclic transition, either the efflux from the DP is too great or the influx from the DS is too little (Figure 1).…”

Section: Hair Today G One Tomorrowmentioning

confidence: 99%

A bald statement — Current approaches to manipulate miniaturisation focus only on promoting hair growth

Pantelireis

2018

Experimental Dermatology

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Hair plays a large part in communication and society with its role changing through time and across cultures. Most people do not leave the house before combing their hair or shaving their beard and for many hair loss or irregular hair growth can have a significant impact on their psychological health. Somewhat unsurprisingly, according to GMR Data, today's global hair care industry is worth an estimated $87 Billion, with hair loss estimated at $2.8 Billion. Considering that no current hair loss-related products can completely reverse hair loss, it is reasonable to believe this market could expand significantly with the discovery of a comprehensive therapy. As such, a great deal of research focuses on overcoming hair loss, and in particular, a common form of hair loss known as androgenetic alopecia (AGA) or male pattern baldness. In AGA, hair follicles miniaturise in a large step change from a terminal to a vellus state. Within this viewpoint article, we discuss how influx and efflux of cells into and out from the dermal papilla (DP) can modulate DP size during the hair cycle. As DP size is positively correlated with the size of the hair fibre produced by a follicle, we argue here that therapies for treating AGA should be developed which can alter DP size, rather than just promote hair growth. We also discuss current therapeutics for AGA and emphasise the importance of using the right model systems to analyse miniaturisation.

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“…Therefore, novel treatment strategies for chronic skin wounds need to be urgently developed and tested; ideally in the human system. For safety, cost, and regulatory reasons, cell-based regenerative medicine approaches, with transplanted autologous adult progenitor cells, have long been viewed as a particularly promising skin-regeneration strategy in this context [ 1 , 10 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Nestin+ progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo

et al. 2019

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The combination of an aging population and an increasing prevalence of diseases associated with impaired-wound healing, including obesity, peripheral vascular disease and diabetes, is likely to result in a dramatic increase in the incidence and prevalence of chronic skin wounds. Indeed, systemic reviews are now not only trying to establish both the prevalence and the often under-estimated socio-economic costs of chronic skin wounds, but most importantly are addressing the impact that chronic wounds have on quality of life. Given the clear need for novel approaches to the management of chronic skin ulceration, ideally developed and tested in the human system in a manner that can be rapidly translated into clinical practice, we examined the effects of multipotent primary human nestin + progenitor cells on human wound healing in an ex vivo model. Human sweat gland-derived nestin + cells demonstrated the capacity to significantly promote two key wound healing parameters, i.e., both reepithelialisation and angiogenesis in experimentally wounded, organ-cultured human skin. The current data further support the use of full-thickness human skin wound-healing models ex vivo to pre-clinically test wound healing-promoting candidate agents. Whilst larger studies are required to substantiate a firm “proof-of-concept,” our preliminary studies encourage further efforts to systemically determine the potential of cell-based regenerative medicine strategies in general, and the use of skin appendage-associated human nestin + cells in particular, as novel treatment strategies for chronic skin ulceration. Electronic supplementary material The online version of this article (10.1007/s00403-019-01889-x) contains supplementary material, which is available to authorized users.

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Hair follicle dermal stem cells and skin‐derived precursor cells: Exciting tools for endogenous and exogenous therapies

Cited by 29 publications

References 28 publications

Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells

A bald statement — Current approaches to manipulate miniaturisation focus only on promoting hair growth

Nestin+ progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo

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