Hahella chejuensis is the etiological agent of a novel red egg disease in tilapia (Oreochromis spp.) hatcheries in Thailand

Senapin, Saengchan; Dong, Ha Thanh; Meemetta, Watcharachai; Siriphongphaew, Akasit; Charoensapsri, Walaiporn; Santimanawong, Wanida; Turner, W. A.; Withyachumnarnkul, Boonsirm; Vanichviriyakit, Rapeepun

doi:10.1016/j.aquaculture.2015.12.013

Cited by 24 publications

(28 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two OTUs (Oceanospirillales_OTU1 and Oceanospirillales_OTU3) related to the genus Kistimonas (family Hahellaceae ) were significantly associated with diseased individuals and, in particular, with diseased tube feet. Related bacteria have been identified as pathogens, including Hahella chejuensis , which was identified as the etiological agent of red egg disease in tilapia hatcheries ( 66 ), and Endozoicomonas elysicola , which is responsible for epitheliocystis in cobia hatcheries ( 67 ). Kistimonas has so far been reported as living in close association with invertebrate hosts ( 56 , 68 , 69 ), and its mode of transmission is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Crown-of-Thorns Sea Star Acanthaster cf. solaris Has Tissue-Characteristic Microbiomes with Potential Roles in Health and Reproduction

Høj

Levy

Baillie

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

Outbreaks of coral-eating crown-of-thorns sea stars (CoTS; Acanthaster species complex) cause substantial coral loss; hence, there is considerable interest in developing prevention and control strategies. We characterized the microbiome of captive CoTS and assessed whether dysbiosis was evident in sea stars during a disease event. Most tissue types had a distinct microbiome. The exception was female gonads, in which the microbiomes were highly variable among individuals. Male gonads were dominated (>97% of reads) by a single Mollicutes-related operational taxonomic unit (OTU). Detailed phylogenetic and microscopy analysis demonstrated the presence of a novel Spiroplasma-related bacterium in the spermatogenic layer. Body wall samples had high relative abundance (43 to 64% of reads) of spirochetes, likely corresponding to subcuticular symbionts reported from many echinoderms. Tube feet were characterized by Hyphomonadaceae (24 to 55% of reads). Pyloric cecal microbiomes had high alpha diversity, comprising many taxa commonly found in gastrointestinal systems. The order Oceanospirillales (genera Endozoicomonas and Kistimonas) was detected in all tissues. A microbiome shift occurred in diseased individuals although differences between tissue types were retained. The relative abundance of spirochetes was significantly reduced in diseased individuals. Kistimonas was present in all diseased individuals and significantly associated with diseased tube feet, but its role in disease causation is unknown. While Arcobacter was significantly associated with diseased tissues and Vibrionaceae increased in diversity, no single OTU was detected in all diseased individuals, suggesting opportunistic proliferation of these taxa in this case. This study shows that CoTS have tissue-characteristic bacterial communities and identifies taxa that could play a role in reproduction and host health.IMPORTANCE Coral-eating crown-of-thorns sea stars (CoTS; Acanthaster species complex) are native to the Indo-Pacific, but during periodic population outbreaks they can reach extreme densities (>1,000 starfish per hectare) and function as a pest species. On the Great Barrier Reef, Australia, CoTS have long been considered one of the major contributors to coral loss. There has been significant investment in a targeted control program using lethal injection, and there is interest in developing additional and complementary technologies that can increase culling efficiencies. The biology of CoTS has been studied extensively, but little is known about their associated microbiome. This cultivation-independent analysis of the CoTS microbiome provides a baseline for future analyses targeting the functional role of symbionts, the identification of pathogens, or the development of reproduction manipulators.

show abstract

Section: Discussionmentioning

confidence: 99%

Crown-of-Thorns Sea Star Acanthaster cf. solaris Has Tissue-Characteristic Microbiomes with Potential Roles in Health and Reproduction

Høj

Levy

Baillie

et al. 2018

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Small samples of spleen and kidney tissue from a clinically diseased fish collected in 2016 were fixed in 2.5% glutaraldehyde in 0.1 M phosphate‐buffered saline, pH 7.4 for 2 hr at 4°C. Post‐fixation with osmium tetroxide, tissue embedding and sectioning with an ultramicrotome were carried out as previously described (Dong, Siriroo et al., ; Senapin et al., ). Sections were examined using a Hitachi‐H500 (20,000‐30,000X magnification) transmission electron microscope operated at 100 kV.…”

Section: Methodsmentioning

confidence: 99%

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

Senapin

Dong

Meemetta

et al. 2018

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2–1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs. Intracytoplasmic inclusion bodies were also observed in the muscle tissue. In a density‐gradient fraction from muscle extracts, TEM revealed enveloped, hexagonal megalocytiviral‐like particles (~100–180 nm). By PCR using primers derived from the Singaporean SDDV genome sequence, four different genes were amplified and sequenced from the Thai isolate revealing 98.7%–99.9% identity between the two isolates. Since viral inclusions were rarely observed, clinical signs and histopathology could not be used to easily distinguish between SDD caused by bacteria or SDDV. We therefore recommend that PCR screening be used to monitor broodstock, fry and grow‐out fish to estimate the current impact of SDDV in Southeast Asia and to prevent its spread.

show abstract

“…Many bacterial and viral diseases have been reported in tilapia by many workers in recent years, especially Flavobacterium columnare (Dong, LaFrentz, Pirarat, & Rodkhum, ), Francisella noatunensis subsp. orientalis (Nguyen, Dong, Senapin, Pirarat, & Rodkhum, ), Hahella chejuensis (Senapin et al, ) and Aeromonas veronii (Dong et al, ), betanodavirus (Keawcharoen et al, ) and infectious spleen and kidney necrosis virus (ISKNV) (Suebsing et al, ).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

Nanthini¹,

Majeed²,

Vimal³

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

Tilapia lake virus (TiLV)‐suspected samples of tilapia were collected from grow‐out ponds located with clinical signs and mortality ranged from 5% to 50%. The reverse transcription–polymerase chain reaction (RT‐PCR) assay revealed the presence of TiLV in the disease outbreak ponds. Cell lines were developed from heart, gill and eye of Mozambique tilapia and characterized. Morphologically, these cell lines are composed of epithelioid cells. The optimum growth of these cells was observed at 28°C and 20% concentration of FBS. After cryopreservation, 70%–90% of cells were found to be viable. The cells of all three cell lines were found to be positive to fibronectin and pancytokeratin. PCR amplification of 16S rRNA and COI of O. mossambicus confirmed the origin of these cell lines from O. mossambicus. Heart and gill cell lines were found to be highly susceptible to TiLV and found to be useful for its isolation from infected fish samples. The experimental infection was carried out in O. niloticus and O. mossambicus using the TiLV propagated in susceptible cell lines. The RT‐PCR results revealed the presence of TiLV in brain, gill, liver, kidney, spleen, eye, muscle, intestine and heart of experimentally infected O. niloticus and O. mossambicus.

show abstract

Hahella chejuensis is the etiological agent of a novel red egg disease in tilapia (Oreochromis spp.) hatcheries in Thailand

Cited by 24 publications

References 14 publications

Crown-of-Thorns Sea Star Acanthaster cf. solaris Has Tissue-Characteristic Microbiomes with Potential Roles in Health and Reproduction

Crown-of-Thorns Sea Star Acanthaster cf. solaris Has Tissue-Characteristic Microbiomes with Potential Roles in Health and Reproduction

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

In vitro propagation of tilapia lake virus in cell lines developed from Oreochromis mossambicus

Contact Info

Product

Resources

About