Haemolytic and cytotoxic action of latarcin Ltc2a

Vorontsova, Olga; Egorova, N. S.; Arseniev, Alexander S.; Feofanov, Аlexey V.

doi:10.1016/j.biochi.2010.09.016

Cited by 39 publications

(30 citation statements)

References 42 publications

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“…While Ltc display poor insecticidal properties, antimicrobial activity of several Ltc against Gram-positive and Gram-negative bacteria falls into the low micromolar concentration range [28]. Erythrocytes and cancer cells, such as erythroleukemia K562, were found to be affected by Ltc [29,30]. In all these cases, membrane-active properties of Ltc were supposed to underlie cytotoxicity.…”

Section: Origin Of Latarcinsmentioning

confidence: 99%

“…The hemolytic effect is especially pronounced for Ltc 2a (Table 4). It was investigated in detail using a fluorescently labeled analog of this peptide [29]. The interaction of Ltc 2a with erythrocytes was found to be a multi-stage process (Fig.…”

Section: Hemolysismentioning

confidence: 99%

“…A good example is short LP isolated from Lachesana tarabaevi spider venom that were called latarcins (Ltc) [28], which we discuss in detail here. They display membrane activity and cytotoxic effects against bacteria [28] and mammalian cells [29,30]. Studies of their interaction with model lipid membranes [31][32][33][34] or with whole cells [29,30] are a well-established methodology for characterization of membrane-active properties in LP.…”

Section: Introductionmentioning

confidence: 99%

“…They display membrane activity and cytotoxic effects against bacteria [28] and mammalian cells [29,30]. Studies of their interaction with model lipid membranes [31][32][33][34] or with whole cells [29,30] are a well-established methodology for characterization of membrane-active properties in LP. Similarly the venom of Cupiennius salei features the presence of numerous cupiennins [35], and Oxyopes takobius produces a number of oxyopinins [36,37] (Table 1).…”

Section: Introductionmentioning

confidence: 99%

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Latarcins: versatile spider venom peptides

Dubovskii

Vassilevski

Kozlov

et al. 2015

Cell. Mol. Life Sci.

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Arthropod venoms feature the presence of cytolytic peptides believed to act synergetically with neurotoxins to paralyze prey or deter aggressors. Many of them are linear, i.e., lack disulfide bonds. When isolated from the venom, or obtained by other means, these peptides exhibit common properties. They are cationic; being mostly disordered in aqueous solution, assume amphiphilic α-helical structure in contact with lipid membranes; and exhibit general cytotoxicity, including antifungal, antimicrobial, hemolytic, and anticancer activities. To suit the pharmacological needs, the activity spectrum of these peptides should be modified by rational engineering. As an example, we provide a detailed review on latarcins (Ltc), linear cytolytic peptides from Lachesana tarabaevi spider venom. Diverse experimental and computational techniques were used to investigate the spatial structure of Ltc in membrane-mimicking environments and their effects on model lipid bilayers. The antibacterial activity of Ltc was studied against a panel of Gram-negative and Gram-positive bacteria. In addition, the action of Ltc on erythrocytes and cancer cells was investigated in detail with confocal laser scanning microscopy. In the present review, we give a critical account of the progress in the research of Ltc. We explore the relationship between Ltc structure and their biological activity and derive molecular characteristics, which can be used for optimization of other linear peptides. Current applications of Ltc and prospective use of similar membrane-active peptides are outlined.

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Section: Origin Of Latarcinsmentioning

confidence: 99%

Section: Hemolysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Latarcins: versatile spider venom peptides

Dubovskii

Vassilevski

Kozlov

et al. 2015

Cell. Mol. Life Sci.

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“…15 Study showed that Ltc2a exhibits in vitro cytotoxicity against human erythroleukemia cells. 16 In another study it was found that Lycosin-1 suppressed the growth of tumor cells in in-vitro and in in-vivo studies.…”

Section: Anticarcinogenic Activitymentioning

confidence: 97%

A Review on Spider Toxin: Changing Poison Into Medicine

Mohapatra¹,

Kafle²,

Panigrahi³

et al. 2018

J Biol Sci Opin

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Perfectly said by Paracelsus that "Solely the dose determines that a thing is not a poison". Previously considered poisons are now considered as the medicines in right dose. As the famous Botulinum toxin which was proved to be neurotoxin, now used as to relief chronic pain, depression, cosmetic etc. Many snake venoms are now used to treat deadly diseases such as cancer etc. This article solely focuses on the spider venoms and its medicinal use.

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