Haemolytic and antibiofilm properties of haemocyanin purified from the haemolymph of Indian white shrimp Fenneropenaeus indicus

Immune indices and identical functions of two prophenoloxidases from the haemolymph of green tiger shrimp Penaeus semisulcatus and its antibiofilm activity

“…Within its superfamily, which poses large groups of proteins, haemocyanin can display different molecular masses (Burmester, 2001). The purified haemocyanins have two distinct subunits, which have been reported in different crustaceans, such as the Kuruma shrimp Marsupenaeus japonicus (67 and 77 kDa) (Sivagamavalli and Vaseeharan, 2015), the Pacific white shrimp Litopenaeus vannamei (75 and 82 kDa) (Figueroa-Soto et al, 1997), the Northern white shrimp Litopenaeus setiferus (77 and 82 kDa) (Brouwer et al, 1978), the mud crab Scylla serrata (70-80 kDa) (Yan et al, 2011) and the giant isopod Bathynomus giganteus (70 and 72 kDa) (Pless et al, 2003); single bands were reported in the mud crab Scylla olivacea (400 kDa) (Chen et al, 2007), as well as in the Indian white shrimp Fenneropenaeus indicus (74 kDa) (Ishwarya et al, 2016). In our study, the haemolymph was collected from P. pelagicus to isolate Pp-Hc by employing gel filtration chromatography.…”

“…Pp-Hc showed maximum killing effect against Gram-negative bacteria if compared to Gram-positive ones. The reason behind this seems to be related to the devoid of a thick peptidoglycan layer in Gram-negative bacteria (Ishwarya et al, 2016). Generally, immune related proteins do not show any target site to damage bacterial cells, they simply perturb the bacterial membrane structure due to their affinity towards it.…”

“…The haemocyanin was purified following the method by Ishwarya et al (2016) with minor changes. To purify haemocyanin from the supernatant of haemolymph, gel filtration chromatography was carried out at 4°C; 20 ml of TBS supernatant was run through Sephadex G-100 matrix, pre-equilibrated with TBS/CaCl 2 buffer.…”

“…Antibiotic resistance now represents a serious concern in both developed and developing nations (Hernández Serrano, 2005;Ventola, 2013). Eco-friendly solutions to fight the growth of multiple antibiotic resistance, resistance transfer to pathogenic bacteria, and reduced efficacy of antibiotic treatment for diseases caused by resistant pathogens, are of timely importance nowadays (Levy, 2002;Ishwarya et al, 2016;Anjugam et al, 2016;Iswarya et al, 2017;Jayanthi et al, 2017). A way to tackle the problem is to shift towards the use of compounds with multiple mechanisms of action for limited periods of time, to avoid prolonged exposures of a given organisms to the same toxic molecules, which in turn leads to grow of resistance to those drugs (Benelli et al, 2017).…”

Molluscan Compounds Provide Drug Leads for the Treatment and Prevention of Respiratory Disease

“…As well, Maselli et al [170] recently reported antibiofilm activity (60% eradication at 80 μM) among peptides derived from the suckers of O vulgaris. Preliminary evidence indicates that Hcs from marine crustaceans can also inhibit biofilm formation (at 0.1 mg/mL) [180][181][182] and molluscan Hcs could function similarly.…”