Haemolymph juvenile hormone esterase during the life cycle of the tobacco hornworm, Manduca sexta (L.)

Jesudason, Princy; Venkatesh, Krishnappa; Roe, R. Michael

doi:10.1016/0020-1790(90)90071-2

Cited by 41 publications

(21 citation statements)

References 36 publications

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“…In the last larval instar, the critical weight marks the initiation of a dramatic change in physiology. After reaching the critical weight, the level of JHE in the hemolymph rises abruptly [21,22] and the JH titer gradually drops to zero. Once JH has disappeared, the secretion of PTTH and ecdysone are disinhibited.…”

Section: Resultsmentioning

confidence: 99%

A quantitative analysis of the mechanism that controls body size in Manduca sexta

Nijhout

Davidowitz

Roff

2006

J Biol

149

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BackgroundBody size is controlled by mechanisms that terminate growth when the individual reaches a species-specific size. In insects, it is a pulse of ecdysone at the end of larval life that causes the larva to stop feeding and growing and initiate metamorphosis. Body size is a quantitative trait, so it is important that the problem of control of body size be analyzed quantitatively. The processes that control the timing of ecdysone secretion in larvae of the moth Manduca sexta are sufficiently well understood that they can be described in a rigorous manner.ResultsWe develop a quantitative description of the empirical data on body size determination that accurately predicts body size for diverse genetic strains. We show that body size is fully determined by three fundamental parameters: the growth rate, the critical weight (which signals the initiation of juvenile hormone breakdown), and the interval between the critical weight and the secretion of ecdysone. All three parameters are easily measured and differ between genetic strains and environmental conditions. The mathematical description we develop can be used to explain how variables such as growth rate, nutrition, and temperature affect body size.ConclusionOur analysis shows that there is no single locus of control of body size, but that body size is a system property that depends on interactions among the underlying determinants of the three fundamental parameters. A deeper mechanistic understanding of body size will be obtained by research aimed at uncovering the molecular mechanisms that give these three parameters their particular quantitative values.

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Section: Resultsmentioning

confidence: 99%

A quantitative analysis of the mechanism that controls body size in Manduca sexta

Nijhout

Davidowitz

Roff

2006

J Biol

149

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“…These two forms of JHDPT may both be present in vivo, or, alternatively, one of them may have arisen artifactually during sample processing. Multiple electrophoretic variants have been reported for the JHE from a single tissue type of this species (Jesudason et al, 1990(Jesudason et al, , 1992Sparks et al, 1989) and several other lepidopterans (e.g., Abdel-Aal et al, 1988;Hammock, 1985;Hanzlik and Hammock, 1987). The use of electrophoretically purified JHDPT indicates a strict dependence upon ATP and Mg2+ for activity.…”

Section: Discussionmentioning

confidence: 96%

Characterization of the juvenile hormone epoxide hydrolase (JHEH) and juvenile hormone diol phosphotransferase (JHDPT) from Manduca sexta Malpighian tubules

Grieneisen

Kieckbusch

Mok

et al. 1995

Arch Insect Biochem Physiol

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Juvenile hormone epoxide hydrolase (JHEH) and juvenile hormone diol phosphotransferase (JHDPT) were characterized from the Malpighian tubules of day 1 fifth instar Manduca sexta. An improved RP-HPLC assay is described for the major metabolites of (lOR,lIS) juvenile hormone I: diol, acid, aciddiol, and diol-phosphate. JHEH is strictly associated with membrane fractions, while JHDPT is cytosolic. JHEH may be solubilized in active form by the nonionic detergents Thesit or MEGA-8. Separation of Malpighian tubule cytosol proteins using preparative isoelectric focusing yields two zones which contain JHDPT activity, at pi 4.8-5.1 and 6.8-8.2. The partially purified JHDPT from either zone requires both ATP and Mg2+ for activity, so this enzyme may be formally called either ATP:juveni/e hormone diol phosphotransferase or juvenile hormone diol kinase (EC 2.1.7.3). Metabolites more polar than JH I aciddiol and JH I diol-phosphate are generated in vivo from either [3H]JH I or 13H]JH I diol. 0 1995 Wiley-Liss, inc.

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“…separata parasitized by A. kariyai is a repressive factor that disrupted normal host metamorphosis. It is well known that juvenile hormone esterase activity in the pupal stage of lepidopterans is maintained at a low level until eclosion (Jesudason et al, 1990). Therefore, parasitoids using the pupal host do not need to produce a repressive factor, such as juvenile hormone esterase.…”

Section: Discussionmentioning

confidence: 99%

Larval development of a larval parasitoid (Apanteles kariyai) forced to oviposit in a pupal host (Pseudaletia separata)

Shimizu

Shiotsuki

Tanaka

et al. 1995

Entomologia Exp Applicata

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Haemolymph juvenile hormone esterase during the life cycle of the tobacco hornworm, Manduca sexta (L.)

Cited by 41 publications

References 36 publications

A quantitative analysis of the mechanism that controls body size in Manduca sexta

A quantitative analysis of the mechanism that controls body size in Manduca sexta

Characterization of the juvenile hormone epoxide hydrolase (JHEH) and juvenile hormone diol phosphotransferase (JHDPT) from Manduca sexta Malpighian tubules

Larval development of a larval parasitoid (Apanteles kariyai) forced to oviposit in a pupal host (Pseudaletia separata)

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