Haemoglobin typing and its variations in Iranian domestic dogs

Atyabi, Nahid; Rahbarghazi‬, Reza; Araghi, Atefeh; Neqouiejahromi, Omid Ali

doi:10.1007/s00580-011-1322-4

Cited by 6 publications

(16 citation statements)

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“…Após análise densitométrica, assim como observado por Atyabi et al (2012), a maior parte dos componentes de hemoglobina se localizaram no final anodo aproximadamente na mesma linha, sendo classificado como HbA, mas em alguns casos, valores baixos de HbA2 também foram observados na avaliação densitométrica com um padrão mais lento de migração. Desta forma, pôde-se dividir em percentuais cada fração de hemoglobina presente nos animais.…”

Section: Resultsunclassified

“…Os resultados obtidos neste estudo são semelhantes aos relatados por Seal (1969), Naik et al (1971) e Atyabi et al (2012), que apenas encontraram frações de HbA em estudos por eletroforese alcalina em cães. No entanto, diferentemente de Atyabi et al (2012), não foi observada a presença de hemoglobina fetal em meio alcalino.…”

Section: Resultsunclassified

“…No entanto, diferentemente de Atyabi et al (2012), não foi observada a presença de hemoglobina fetal em meio alcalino. No atual estudo foram avaliados os perfis eletroforéticos apenas de animais em idade reprodutiva, enquanto estes autores avaliaram animais a partir dos quatro meses de idade.…”

Section: Resultsunclassified

“…Esta diferença de genótipos de hemoglobina pode estar associada à seleção de raças mais regional e apurada. No entanto, além de Tanabe et al (1978), em nenhum outro trabalho foi descrito um polimorfismo com dois tipos de hemoglobina em cães (Seal 1969, Kitchen & Brett 1974, Atyabi et al 2012.…”

Section: Resultsunclassified

“…Diversos polimorfismos de hemoglobinas já foram descritos em espécies animais, como bovinos, bubalinos, ovinos, caprinos e felinos (Ramos et al 1997, Regis 2002, Ayres et al 2005, Lacerda & Soto-Blanco 2006, Esharatkhah et al 2007, Barca Júnior et al 2008, Ayres et al 2009, Al-Samarrae et al 2010, Luz et al 2010, Ayres et al 2011, Rego et al 2011, Atyabi et al 2012, Egena & Alao 2014, Pal & Mummed 2014, Yakubu et al 2014. No entanto, não há na literatura relatos de hemoglobinopatias envolvendo cães, e os estudos sobre o polimorfismo das hemoglobinas nesta espécie são escassos.…”

Section: Introductionunclassified

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Pesquisa de hemoglobinopatias em cães da região metropolitana do Rio de Janeiro portadores de anemia crônica

et al. 2017

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RESUMO: Talassemias e hemoglobinopatias são condições hereditárias encontradas em humanos de todo o mundo. Em medicina veterinária, o polimorfismo de hemoglobinas tem sido estudado em animais de produção, mas não existem relatos de hemoglobinopatias em cães, e os estudos envolvendo o polimorfismo de hemoglobinas nesta espécie são escassos. Com o objetivo de pesquisar variantes da hemoglobina em cães, foram coletadas amostras de sangue de 202 caninos de variadas raças, sendo 130 portadores de anemia crônica (Grupo Experimental) e 72 animais clinicamente saudáveis (Grupo Controle). Estas amostras foram submetidas à eletroforese alcalina de hemoglobinas, que permitiu a separação e quantificação das frações de hemoglobina por densitometria, e posteriormente submetidas à eletroforese de hemoglobinas em meio ácido, técnica utilizada em medicina humana para a separação de frações de hemoglobinas variantes que não se diferenciam em meio alcalino. O eritrograma e índices hematimétricos foram obtidos concomitantemente. Os métodos utilizados demonstraram que a HbA é o maior componente da hemoglobina canina, e que uma pequena quantidade de HbA2 pode ser detectada em uma parcela dos animais avaliados, sendo que a maioria dos caninos apresentava exclusivamente HbA em sua composição. Concluiu-se que a presença ou ausência de HbA2 não interfere nos índices hematimétricos dos animais avaliados, e que quando comparadas as hemoglobinas dos grupos Experimental e Controle, não são observadas diferenças na distribuição das frações destas, além de não serem observadas hemoglobinas variantes nos caninos avaliados.

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Section: Resultsunclassified

Section: Introductionunclassified

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Pesquisa de hemoglobinopatias em cães da região metropolitana do Rio de Janeiro portadores de anemia crônica

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Validation, reference intervals and overlap performance of a new commercially available automated capillary electrophoresis assay for the determination of the major fraction of glycated haemoglobin (HbA1c) in dogs

Oikonomidis

Tsouloufi

Soubasis

et al. 2018

The Veterinary Journal

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Haemoglobin A1c (HbA1c), the major fraction of glycated haemoglobin, is widely used for the diagnosis and monitoring of diabetes mellitus in human beings. However, there is a paucity of literature on the most reliable methods available for measurement of canine HbA1c. The aim of this study was to validate a new automated capillary electrophoresis assay for canine HbA1c, to generate a reference interval and to assess the overlap performance of the assay for the diagnosis of diabetes mellitus. Eighty-three blood samples treated with EDTA were included in the study, comprising 63 from healthy dogs and 20 from diabetic dogs. Linearity was assessed by mixing canine samples of known HbA1c percentage in different proportions, precision was assessed by repeated (n=8) measurement of five canine samples, and stability was assessed by measuring canine samples stored at 4°C for 96h and -20°C for 4 weeks. The robust method was used to determine the reference interval. The assay was demonstrated to be linear (R=0.943). Intra-assay and inter-assay coefficients of variation (CVs) were 4.8% and 7.0%, respectively. CVs for blood samples stored at 4°C and -20°C were 7.2% and 11.2%, respectively. The reference interval was 0.6-2.7%. Dogs with diabetes mellitus had significantly (P<0.001) higher mean HbA1c (5.24±0.88%) compared to the reference population (1.64±0.55%), with no overlap between results. A HbA1c cut-off of 3.3% clearly differentiated diabetic from healthy dogs. The capillary electrophoresis assay was properly validated for canine HbA1c and the reference interval was determined, while the overlap performance of the assay was excellent.

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Capillary hemoglobin electrophoresis of healthy and anemic dogs: quantification, validation, and reference intervals of hemoglobin fractions

Oikonomidis

Tsouloufi

Mylonakis

et al. 2019

Preprint

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27Despite the advances in canine medicine and the rapid gaining of attention of canine 28 models in biomedical field and particularly in hemoglobin genes research, the studies on canine 29 hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate 30 the electrophoretic pattern of canine hemoglobin and the possible effect of age, sex, and anemia 31 using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference 32 intervals (RIs) for canine hemoglobin fractions. Blood samples were collected from 53 healthy 33 and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-34 piercing was used for hemoglobin analysis and it was validated using canine blood samples. R 35 statistical language was employed for the statistical analyses. A major hemoglobin fraction 36 (named HbA 0 ) and a minor one (named HbA 2 ) were identified in 100% and 47.4% of samples, 37 respectively. The within-run and between-run CV was 0.1% for HbA 0 and 9.1% and 11.2% for 38 HbA 2 , respectively. The extremely narrow range of HbA 0 and HbA 2 values hampered a 39 linearity study using canine blood samples. The RIs for HbA 0 and HbA 2 were 98.9-100% and 40 0-1.1%, respectively. HbA 0 and HbA 2 values were not correlated with age (P=0.866). No 41 differences were observed in the median HbA 0 and HbA 2 between the two sexes (P=0.823), 42 and healthy and anemic dogs (P=0.805). In conclusion, the capillary electrophoresis revealed 43 a major hemoglobin fraction and an inconsistently present minor fraction. No effect of age, 44 sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be 45 suitable for use in future investigations. 46 47 48 49 50 3 51 6 125brief, the EDTA-treated whole blood sample is diluted with a hemolysing solution and the 126 resulting solution is then hydrodynamically injected at the anodic end of the capillary. A 127 constant, high voltage is applied for 8 min, which allows the migration and separation of the 128 hemoglobin variants. These are then directly detected by spectrophotometry (415 nm) and the 129 electrophoretograms are automatically generated. The total output time is approximately 20 130 min for the first run and 12 min for every other run. 131The validation of the analyzer was initially designed to include linearity, repeatability, 132 and reproducibility. The repeatability or within-run precision was evaluated using blood 133 samples from three dogs. Each sample was measured eight times in succession and the 134 coefficient of variation (CV) was calculated. Blood samples from the same three dogs were 135 used for the evaluation of reproducibility or between-run precision. Six aliquots were made 136 from each sample and were measured over a period of 3 days; then, the CV was calculated. 137The distribution of data was assessed using the Shapiro-Wilk test. The 95% reference 138 intervals (RIs) were calculated using the non-parametric method, while the 90% confidence 139 intervals (CIs) for ...

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Haemoglobin typing and its variations in Iranian domestic dogs

Cited by 6 publications

References 8 publications

Pesquisa de hemoglobinopatias em cães da região metropolitana do Rio de Janeiro portadores de anemia crônica

Pesquisa de hemoglobinopatias em cães da região metropolitana do Rio de Janeiro portadores de anemia crônica

Validation, reference intervals and overlap performance of a new commercially available automated capillary electrophoresis assay for the determination of the major fraction of glycated haemoglobin (HbA1c) in dogs

Capillary hemoglobin electrophoresis of healthy and anemic dogs: quantification, validation, and reference intervals of hemoglobin fractions

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