Haemoglobin, oxygen carriers and perioperative organ perfusion

Kocián, Roman; Spahn, Donat R.

doi:10.1016/j.bpa.2007.10.003

Cited by 35 publications

(14 citation statements)

References 141 publications

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“…In fact, several classes of perfluorocarbon molecules have been approved by the Food and Drug Administration (FDA) for study in clinical trials as artificial blood substitutes because of their oxygen-carrying properties (47). However, none of these products is formulated for labeling cells in culture.…”

Section: Discussionmentioning

confidence: 99%

Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking

et al. 2010

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Background aims Dendritic cells (DC) are increasingly being used as cellular vaccines to treat cancer and infectious diseases. While there have been some promising results in early clinical trials using DC-based vaccines, the inability to visualize non-invasively the location, migration and fate of cells once adoptively transferred into patients is often cited as a limiting factor in the advancement of these therapies. A novel perflouropolyether (PFPE) tracer agent was used to label human DC ex vivo for the purpose of tracking the cells in vivo by 19F magnetic resonance imaging (MRI). We provide an assessment of this technology and examine its impact on the health and function of the DC. Methods Monocyte-derived DC were labeled with PFPE and then assessed. Cell viability was determined by examining cell membrane integrity and mitochondrial lipid content. Immunostaining and flow cytometry were used to measure surface antigen expression of DC maturation markers. Functional tests included bioassays for interleukin (IL)-12p70 production, T-cell stimulatory function and chemotaxis. MRI efficacy was demonstrated by inoculation of PFPE-labeled human DC into NOD-SCID mice. Results DC were effectively labeled with PFPE without significant impact on cell viability, phenotype or function. The PFPE-labeled DC were clearly detected in vivo by 19F MRI, with mature DC being shown to migrate selectively towards draining lymph node regions within 18 h. Conclusions This study is the first application of PFPE cell labeling and MRI cell tracking using human immunotherapeutic cells. These techniques may have significant potential for tracking therapeutic cells in future clinical trials.

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Section: Discussionmentioning

confidence: 99%

Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking

et al. 2010

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“…In experimental models of severe HS, resuscitation with some haemoglobin-based oxygen carriers (HBOC) decreases lactic acidosis and improves tissue oxygenation, haemodynamics, and survival. [1][2][3][4][5][6] This is of particular interest when blood is unavailable. 2 However, adverse events, including myocardial infarctions, related to systemic and pulmonary vasoconstriction were reported in HBOC clinical trials.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6] This is of particular interest when blood is unavailable. 2 However, adverse events, including myocardial infarctions, related to systemic and pulmonary vasoconstriction were reported in HBOC clinical trials. 7,8 Indeed, increased afterload from systemic vasoconstriction, and ventilation-perfusion mismatch causing blood deoxygenation and decreased venous return and cardiac output from pulmonary vasoconstriction could theoretically contribute to cardiac ischemia.…”

Section: Introductionmentioning

confidence: 99%

Sodium nitroprusside ameliorates systemic but not pulmonary HBOC-201-induced vasoconstriction: An exploratory study in a swine controlled haemorrhage model

et al. 2012

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“…The standard therapy for acute symptomatic anemia is allogeneic blood transfusion 1 . Severe anemia (generally a hemoglobin [Hb] level of <5 g/dL, although the exact threshold is a matter of debate and depends on several factors) compromises the oxygen‐carrying capacity of blood and can lead to ischemia and multiorgan failure.…”

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confidence: 99%

Management of acute anemia in a Jehovah's Witness patient with acute lymphoblastic leukemia with polymerized bovine hemoglobin‐based oxygen carrier: a case report and review of literature

et al. 2010

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Haemoglobin, oxygen carriers and perioperative organ perfusion

Abstract: Haemoglobin, oxygen carriers and perioperative organ perfusion Kocian, R; Spahn, D R Spahn, D R (2008 1 AbstractUnder normal conditions, only 20-30% of the delivered oxygen is metabolized. In

Cited by 35 publications

References 141 publications

Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking

Functional assessment of human dendritic cells labeled for in vivo 19F magnetic resonance imaging cell tracking

Sodium nitroprusside ameliorates systemic but not pulmonary HBOC-201-induced vasoconstriction: An exploratory study in a swine controlled haemorrhage model

Management of acute anemia in a Jehovah's Witness patient with acute lymphoblastic leukemia with polymerized bovine hemoglobin‐based oxygen carrier: a case report and review of literature

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