HAEMOCYTES OF THE FRESHWATER MUSSEL, <i>DREISSENA POLYMORPHA</i> PALLAS: CYTOLOGY, CYTOCHEMISTRY AND X-RAY MICROANALYSIS

Giambérini, Laure; Auffret, Michel; Pihan, Jean-Claude

doi:10.1093/mollus/62.3.367

Cited by 41 publications

(25 citation statements)

References 33 publications

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“…Zebra mussels, Dreissena polymorpha, were chosen as the test organism because of their known ecological impacts [Heath et al, 1995;Raikow et al, 2004] and their interactions with cyanobacterial blooms [Vanderploeg et al, 1996[Vanderploeg et al, , 2001Juhel et al, 2006a]. Furthermore, as the haemocytes of these bivalves flow freely in the bivalve's body [Giamberini et al, 1996], they are likely to be exposed directly to MCs, even if the toxins, present in the cyanobacteria, do not pass through the mussels' gut.…”

Section: Discussionmentioning

confidence: 99%

In vivo exposure to microcystins induces DNA damage in the haemocytes of the zebra mussel, Dreissena polymorpha, as measured with the comet assay

Juhel

O’Halloran

Culloty

et al. 2006

Environ and Mol Mutagen

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The Comet assay was used to investigate the potential of the biotoxin microcystin (MC) to induce DNA damage in the freshwater zebra mussel, Dreissena polymorpha. Mussels maintained in the laboratory were fed daily, over a 21-day period, with one of four strains of the cyanobacterium, Microcystis aeruginosa. Three of the strains produced different profiles of MC toxin, while the fourth strain did not produce MCs. The mussels were sampled at 0, 7, 14, and 21 days by withdrawing haemocytes from their adductor muscle. In addition, a positive control was performed by exposing a subsample of the mussels to water containing cadmium chloride (CdCl(2)). Cell viability, measured with the Fluorescein Diacetate/Ethidium Bromide test, indicated that the MC concentrations, to which the mussels were exposed, were not cytotoxic to the haemocytes. The Comet assay performed on the haemocytes indicated that exposure to CdCl(2) produced a dose-responsive increase in DNA damage, demonstrating that mussel haemocytes were sensitive to DNA-damaging agents. DNA damage, measured as percentage tail DNA (%tDNA), was observed in mussels exposed to the three toxic Microcystis strains, but not in mussels exposed to the nontoxic strain. Toxin analysis of the cyanobacterial cultures confirmed that the three MC-producing strains exhibit different toxin profiles, with the two MC variants detected being MC-LF and MC-LR. Furthermore, the DNA damage that was observed appeared to be strain-specific, with high doses of MC-LF being associated with a higher level of genotoxicity than low concentrations of MC-LR. High levels of MC-LF also seemed to induce relatively more persistent DNA damage than small quantities of MC-LR. This study is the first to demonstrate that in vivo exposure to MC-producing strains of cyanobacteria induces DNA damage in the haemocytes of zebra mussels and confirms the sublethal toxicity of these toxins.

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Section: Discussionmentioning

confidence: 99%

In vivo exposure to microcystins induces DNA damage in the haemocytes of the zebra mussel, Dreissena polymorpha, as measured with the comet assay

Juhel

O’Halloran

Culloty

et al. 2006

Environ and Mol Mutagen

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“…In freshwater mussels, podocytes contain Ca, Mg, P, and S combined, to a lesser extent, with several trace elements such as Cu, Fe, Pb, and Zn in the matrix of lysosomes (Giamberini and Pihan, 1996;Giamberini et al, 1996a). The colocalization of Pb with Ca, P, and S has been confirmed by X-ray mapping (Giamberini et al, 1996b).…”

Section: Cell-mediated Transportmentioning

confidence: 94%

“…It is worth noting that hemocytes contain more than 90% of the body Cu and Zn Thomson et al, 1985). In zebra mussel, granulocytes are involved in metal sequestration and transport (Giamberini et al, 1996a). EPMA revealed that Pb is localized in lysosomes combined mainly with Ca, S, and P and other minor trace elements.…”

Section: Cell-mediated Transportmentioning

confidence: 99%

“…In addition, hemocytes and rhogocytes (pore/brown cells) participate directly in metal elimination, being released by diapedesis across the epithelia of the mantle (Robinson and Ryan, 1988), digestive tract (Marigómez et al, 1990b;Giamberini et al, 1996a;, and kidney (Marigó-mez et al, 1990a,c). In winkles, besides diapedesis of complete hemocytes, semidigested hemocytes surrounded by membrane structures have been observed in nephrocytes (Marigómez et al, 1990c).…”

Section: Elimination Of Metalsmentioning

confidence: 99%

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Cellular and subcellular distribution of metals in molluscs

Marigómez

Soto

Cajaraville

et al. 2002

Microscopy Res & Technique

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414

202

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The cellular processes involved in metal metabolism in molluscs are reviewed, with emphasis on the contribution of microscopy (AMG, ARG, EPMA, and SIMS) to both basic research of metal cell biology and applied environmental research. In molluscs, metal uptake may occur by facilitated diffusion, active transport, or endocytosis, and can be enhanced by MT synthesis or formation of mineralized granules. In aquatic molluscs, gills constitute a key interface for dissolved metal uptake, where metals are bound to MT, incorporated into lysosomes, and released basally towards the blood plasma and circulating hemocytes. However, particulate metal uptake is mainly achieved via the digestive tract by endocytosis; further metals are transferred first to lysosomes and then to residual bodies, especially in the digestive cells of the digestive gland. Additionally, metals can be accumulated selectively in specific cell types. As ligands pools differ from cell to cell, different metals may be retained in different cell types. Class "a" metals are localized in cells with granules composed of carbonate, oxalate, phosphate, and sulfate (oxygen donors), whereas "b" metals are associated with those cell types rich in sulfur and nitrogen ligands (sulfur donors). In molluscs, oxygen donors occur in connective tissue calcium cells and basophilic cells, whereas sulfur donors are present in digestive cells, podocytes, nephrocytes, and rhogocytes. Hemocytes, which constitute the most relevant system for metal transport between tissues, move around the body and may penetrate tissues and remove metals from the inner medium to be accumulated in lysosomes as nondigested products. Rhogocytes also participate in metal mobilization, accumulation, and release. The assessment of metal levels in target cells of sentinel molluscs by microscopic techniques provides an early-warning measure, with promising applications as an exposure biomarker for environmental monitoring programs.

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“…As a result, the literature dealing with bivalve haemocyte studies has increased during the last two decades. Most of these publications use microscopy for morphological analysis (Seiler & Morse, 1988;Au#ret, 1989;Hine & Wesney, 1994;Giamberini et al, 1996;Carballal et al, 1997;Lopez et al, 1997;Nakayama et al, 1997), and functional analysis (e.g. phagocytosis) (Hinsch & Hunte, 1990;Tripp, 1992;Mourton et al, 1992;Fryer & Bayne, 1996;Mortensen & Glette, 1996).…”

mentioning

confidence: 99%

Flow cytometric analysis of European flat oyster, Ostrea edulis, haemocytes using a monoclonal antibody specific for granulocytes

Renault

Xue

Chilmonczyk

2001

Fish & Shellfish Immunology

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HAEMOCYTES OF THE FRESHWATER MUSSEL, DREISSENA POLYMORPHA PALLAS: CYTOLOGY, CYTOCHEMISTRY AND X-RAY MICROANALYSIS

Cited by 41 publications

References 33 publications

In vivo exposure to microcystins induces DNA damage in the haemocytes of the zebra mussel, Dreissena polymorpha, as measured with the comet assay

In vivo exposure to microcystins induces DNA damage in the haemocytes of the zebra mussel, Dreissena polymorpha, as measured with the comet assay

Cellular and subcellular distribution of metals in molluscs

Flow cytometric analysis of European flat oyster, Ostrea edulis, haemocytes using a monoclonal antibody specific for granulocytes

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