Haematology, cytochemical and ultrastructural characteristics of blood cells in leopard (Panthera pardus)

Salakij, Chaleow; Salakij, Jarernsak; Narkkong, Nual-Anong; Prihirunkit, Kreangsak; Kamolnorranath, S.; Apibal, S

doi:10.1007/s00580-008-0774-7

Cited by 11 publications

(17 citation statements)

References 6 publications

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“…This may be homeostatic or due to epinephrine release 12 secondary to physical restraint using nets. The MCV of RBCs in Leopard Cats was the same as in leopards (mean MCV 44 fL, n =17), 7 flat‐headed cats (mean MCV 46 fL, n =2), 5 and domestic cats (range, 39–55 fL), 11 but smaller than that of fishing cats (mean MVC 63 fL, n =13) 4 and clouded leopards (mean MCV 59 fL, n =17) 6 . The blunt‐end crenation of RBCs and the Heinz bodies detected in the Leopard Cats are also frequently found in domestic cats 11,12…”

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 72%

“…The RBC mass of Leopard Cats was higher than that of fishing cats (mean PCV 0.41 L/L, n =13), 4 clouded leopards (mean PCV 0.36 L/L, n =17), 6 leopards (mean PCV 0.34 L/L, n =17), 7 and domestic cats 11 . This may be homeostatic or due to epinephrine release 12 secondary to physical restraint using nets.…”

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 86%

“…Cytochemical staining is useful for comparing leukocyte differences both within and between feline species and for characterizing leukemic cells 3 . We previously evaluated morphologic, ultrastructural, and cytochemical staining features of blood cells in captive, clinically healthy fishing cats, 4 flat‐headed cats, 5 clouded leopards, 6 and leopards 7 . Reports of blood cell features in Leopard Cats are limited 8 .…”

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 99%

“…EDTA samples from 6 Leopard Cats were processed for electron microscopy as described previously 5–7 . For scanning electron microscopy (SEM), a drop of fresh blood was fixed in 1.5% glutaraldehyde in 0.1 M phosphate buffer (PB), pH 7.3, at 4°C overnight.…”

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 99%

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Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis)

Salakij

Salakij²,

Prihirunkit³

et al. 2010

Veterinary Clinical Pathol

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Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 72%

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 86%

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 99%

Section: Hemotologic Results In 12 Leopard Catsmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis)

Salakij

Salakij²,

Prihirunkit³

et al. 2010

Veterinary Clinical Pathol

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“…During the last 30 years, the use of blood profiles to estimate the welfare of wild cats in both captive and wild settings has become a usual practice among zoo veterinaries and field zoologists. At present, for 17 of the 38 wild cat species blood profiles have been obtained and described in scientific literature (Akuzawa, Mochizuki, & Yasuda, ; Brown, Lappin, Brown, Munkhtsog, & Swanson, ; Caro et al., ; Dunbar, Nol, & Linda, ; Erasmus, ; Kocan, Blouin, & Glenn, ; Marco, Martinez, Pastor, & Lavin, ; Prihirunkit, Salakij, Apibal, & Narkkong, ; Salakij, Salakij, Narkkong, Sirinarumitr, & Pattanarangsan, ,b, b, , , and others). However, blood profiles can be influenced significantly by the sampling procedure, including capture method, type of anesthesia, and time to blood collection (Moen, Rasmussen, Burdett, & Pelican, ; Serieys et al., ).…”

Section: Introductionmentioning

confidence: 99%

The method matters: The effect of handling time on cortisol level and blood parameters in wild cats

et al. 2018

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Blood analysis has recently become a popular tool to assess the welfare of the wild cats. However, the estimates of blood parameters may depend on the sampling method. We have tested (1) if the sampling procedure influences blood parameters and (2) what parameters are the most efficient in assessing the physiological status in wild cat species. We assessed the effect of handling time on red blood cells (RBC) and white blood cells (WBC) counts, the ratio of neutrophils to lymphocytes (N/L ratio), and serum cortisol level within 1 hr after the capture of the animal in six far-east wild cats (Prionailurus bengalensis euptilura). Also, we analyzed literature data in 17 cat species to assess the effect of place of study, type of immobilization, and handling time on WBC count and N/L ratio. Serum cortisol level varied significantly with the handling time. RBC and WBC counts were strongly affected by the handling time. N/L ratio was very robust and did not depend on the handling time. However, the analysis of literature data has shown that the prolonged handling time (over 1 hr) and the type of immobilization significantly influence the N/L ratio, whereas the WBC count does not depend on any of considered factors. We conclude that while most blood parameters of cats are affected by routine handling time, the N/L ratio does not vary if the samples are collected within 1 hr after the capture of the animal. All other tested parameters should be treated with caution.

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Observations on the cytomorphology and ultrastructure of the peripheral blood cells of native cattle (Zobawng) of Mizoram, India

Sarkar

Kalita

Choudhary

et al. 2022

Microscopy Res & Technique

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This study aims to characterize the cytomorphology and ultrastructure of blood cells of native cattle of Mizoram. Twelve numbers of blood samples (10 ml) were collected from the Zobawng cattle, irrespective of sex. Blood smears were prepared and stained with different stains for cytomorphological study. The standard protocol has been followed for preparing blood samples for electron microscopy. Under a light microscope, erythrocytes of cattle were non-nucleated and round. The neutrophils were round, and the cytoplasm contained cytoplasmic granules. The eosinophils were rounded in outline with distinct cytoplasmic granules. The presence of basophils was infrequent with distinct blue color cytoplasmic granules. Small, medium, and large types of lymphocytes were recorded. The monocytes were round to oval in outline. Platelets were irregular to round. The reticulocytes were recorded occasionally, like small blue thin rods or granules. The cytoplasm and nucleus of granulocytes fluoresced greenish-yellow and red, respectively, with supravital stain. Under scanning electron microscopy (SEM), the erythrocytes appeared as biconcave discs. Different leukocytes were observed with their finger-like, plate-like, and narrow cell processes on their surface. Platelets were irregular structures. In transmission electron microscopy (TEM), the erythrocytes appeared anucleated biconcave elongated, neutrophils were roughly rounded with small cytoplasmic processes, and eosinophils were roughly circular with small cytoplasmic processes, the basophils were roughly circular with oval to elongated cytoplasmic granules, lymphocytes were roughly circular with centrally placed well-marked oval indented nucleus and some cytoplasmic processes, monocytes appeared spherical with long thick cytoplasmic processes and the non-nucleated platelets appeared roughly round to elongated. Research Highlights• Blood tests are a very good tool for determining animal health and diagnosing hematological illnesses.• This study showed the cytomorphological and ultrastructural features of the blood cells of native cattle (Zobawng) of Mizoram state of India.Rupan Sarkar and Om Prakash Choudhary contributed equally to this manuscript

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Haematology, cytochemical and ultrastructural characteristics of blood cells in leopard (Panthera pardus)

Cited by 11 publications

References 6 publications

Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis)

Characterization of blood cells in the Leopard Cat (Prionailurus bengalensis)

The method matters: The effect of handling time on cortisol level and blood parameters in wild cats

Observations on the cytomorphology and ultrastructure of the peripheral blood cells of native cattle (Zobawng) of Mizoram, India

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