Haematological Profile and Some Reproductive Indices of Male Albino Rats Treated with Ethanolic Stem Bark Extract of Picralima nitida

Abstract

Aim:To evaluate the effects of graded doses of ethanolic stem bark extract of Picralima nitida on haematological profile, some serum reproductive hormones, sperm reserves and testicular histomorphology of male albino rats. Study Design: Thirty male albino rats, weighing between 120 and 200 grams were randomly assigned into six groups of five rats each. Group A received 0.5 ml of distilled water (normal control). Groups B, C and D received 100, 200 and 400 mg/kg BW of the extract respectively. Group E received 2.5 mg/kg BW of CdCl 2 (negative control), while Group F received 5 mg/kg BW of Sildenafil (positive control); orally every other day for six weeks. Place and Duration of Study: Methodology: At the end of the experiment, blood samples were collected for haematology and Mbegbu et al.; JAMPS, 14(1): 1-14, 2017; Article no.JAMPS.34359 2 serology, whereas testicular sections were collected for histopathology following the standards procedures. Data obtained were analyzed by One-way ANOVA. Results: The result showed that all doses of the extract increased neutrophil count, but decreased (P < 0.05) monocyte and RBC counts; 200 and 400 mg/kg BW of the extract decreased (P < 0.05) PCV values; and only 400 mg/kg of the extract reduced (P < 0.05) HB values. 200 and 400 mg/kg BW of the extract, and 2.5 mg/kg BW of CdCl 2 decreased (P < 0.05) serum testosterone and sperm counts. The normal control, 100 mg/kg BW of the extract and 5 mg/kg BW of Sildenafil had normal testicular histoarchitecture; whereas 200 and 400 mg/kg BW of the extract, and 2.5 mg/kg BW of CdCl 2 had slight, moderate and severe degeneration of the germinal epithelium and interstitium, with depletion of sperm cells in the lumen of the seminiferous tubules respectively. Conclusion: This study revealed that treatment with ethanolic stem bark extract of Picralima nitida above 100 mg/kg BW adversely affected haematological and reproductive parameters in male albino rats. Original Research Article

Aqueous Allium sativum (garlic) extract ameliorated CdCl2- induced alterations in blood formation and spermatogenesis in albino rats

“…This was done using the method described by [12], with slight modification [13]. The testicles were excised and trimmed of fats and extraneous tissues.…”

“…The cauda epididymis or the testis was macerated and mixed with 10 ml of phosphate buffer saline solution of PH 6.8.and filtered.0.1 mL of the homogenate was then diluted with 0.9 mL of WBC fluid and 20 μL was viewed using Leica microscope (x10). Estimation of sperm cells numbers was done as previously described [13].…”

Aqueous Allium sativum (garlic) extract ameliorates cadmium chloride-induced alterations in blood formation and spermatogenesis in albino rats

“…The cauda epididymis or the testis was macerated and mixed with 10 ml of phosphate buffer saline solution of PH 6.8.and filtered.0.1 mL of the homogenate was then diluted with 0.9 mL of WBC fluid and 20 μL was viewed using Leica microscope (x10). Estimation of sperm cell numbers was done as previously described [13].…”