Haematological and phenoloxidase activity changes in the shrimpPenaeus stylirostrisin relation with the moult cycle: protection against vibriosis

Moullac, Gilles Le; Groumellec, Marc Le; Ansquer, Dominique; Froissard, Sébastien; Levy, Peva; Aquacop,

doi:10.1006/fsim.1996.0077

Cited by 167 publications

(100 citation statements)

References 14 publications

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“…It has been reported that certain crustacean hemocytes may change their shape and degranulate rapidly in vitro (Soderhall & Smith 1983, Martin & Graves 1985, Soderhall & Cerenius 1992, Rodriguez et al 1995), but we solved this problem by the use of fixed hemolymph which minimized disruption of cellular integrity. Also, it has been reported that the relative percentage of the 3 types of hemocytes do not show any appreciable changes during the entire molt cycle (Tsing et al 1989, Le Moullac et al 1997). Therefore the above considerations do not affect our interpretation of the changes in hemocyte ratio.…”

Section: Discussionmentioning

confidence: 98%

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White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

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White spot syndrome virus (WSSV) was specifically detected by PCR in Penaeus merguiensis hemocytes, hemolymph and plasma. This suggested a close association between the shrimp hemolymph and the virus. Three types of hemocyte from shrimp were isolated using flow cytometry. Dynamic changes of the hemocyte subpopulations in P. merguiensis at different times after infection were observed, indicating that the WSSV infection selectively affected specific subpopulations. Immunofluorescence assay (IFA) and a Wright-Giemsa double staining study of hemocyte types further confirmed the cellular localization of the virus in the infected hemocytes. Electron microscopy revealed virus particles in both vacuoles and the nucleus of the semigranular cells (SGC), as well as in the vacuoles of the granular cells (GC). However, no virus could be detected in the hyaline cells (HC). Our results suggest that the virus infects 2 types of shrimp hemocytes -GCs and SGCs. The SGC type contains higher virus loads and exhibits faster infection rates, and is apparently more susceptible to WSSV infection.

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Section: Discussionmentioning

confidence: 98%

“…Slides were fixed with acetone for 5 min and stained with WrightGiemsa in the dark for 30 min at room temperature. Finally, the slides were washed and rinsed with acetone and xylene (Le Moullac et al 1997), respectively. Aqua Poly/Mount (Polysciences) mounted slides were observed under a phase-contrast microscope.…”

Section: Methodsmentioning

confidence: 99%

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Wang¹,

Liu²,

Seah³

et al. 2002

Dis. Aquat. Org.

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“…The coagulation time of the haemolymph was determined by the capillary method [21]. Total haemocyte count (cells ml ÿ1 ) was performed using a Burker haemocytometer [22]. The haemocytes on the haemocytometer were observed under phase contrast microscope and counted manually in all 25 squares (¼ 0.1 mm 3 ).…”

Section: Haematological Analysismentioning

confidence: 99%

Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes

Citarasu

Sivaram²,

Immanuel³

et al. 2006

Fish & Shellfish Immunology

209

101

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“…Mortality was recorded over a 3 d period. Total haemocyte counts (THC) were performed as described by Le Moullac et al (1997) on surviving C-stage animals at the end of this 3 d period. When mortality was excessive, a similar assessment of THC was conducted after a shorter period post injection.…”

Section: Introductionmentioning

confidence: 99%

Toxic factors of Vibrio strains pathogenic to shrimp

Goarant¹,

Herlin²,

Brizard³

et al. 2000

Dis. Aquat. Org.

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ABSTRACT. Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V. penaeicida, l/. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus styljrostns) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20°C) and not at higher temperature (30°C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria.

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Haematological and phenoloxidase activity changes in the shrimpPenaeus stylirostrisin relation with the moult cycle: protection against vibriosis

Cited by 167 publications

References 14 publications

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis

Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes

Toxic factors of Vibrio strains pathogenic to shrimp

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