Haematological and Biochemical Responses of Juvenile Malabar Blood Snapper (Lutjanus malabaricus Bloch & Schneider, 1801) Exposed to Different Rearing Temperatures and Diets

Abstract

This study is conducted to examine the impacts of different rearing temperature and diet variation on haematological and serum biochemical parameters on the juvenile Lutjanus malabaricus, a commercially important fish species in were divided into eight treatments (4 temperatures × 2 diets) each with three replicates of five juveniles each. Fish were subjected to four different rearing temperatures (22, 26, 30 and 34°C) and fed with a commercial pellet and a natural shrimp diet. After 30 days of experimental period, blood samples were collected and transferred into two different tubes from each sample: One containing EDTA as an anticoagulant agent and the other without EDTA for serum collection. All the haematological and biochemical parameters in all the treatments were within the normal range. Changing temperature and diet led to a significant change (p<0.05) in the total length (TL), body weight (BW), red blood cell (RBC), haemoglobin (HB), haematocrit (HC), thrombocyte (TC) and white blood cell (WBC) but the change in mean corpuscular volume (MCV), mean cell haemoglobin (MCH) while mean cell haemoglobin concentration (MCHC) were not significant (p>0.05). The fish reared at 26°C and 30°C had significantly higher values than those reared at 22°C and 34°C (p<0.05). Total protein (TP), albumin (ALB), albumin/globin ration (A/G ratio), alkaline phosphatase (AP), aspartate aminotransferase (AST) and alanine transaminase (ALT) level were also significantly increased at 30°C and 26°C compared to that of 34°C and 22°C. Globulin (GLB) level showed the opposite trend. In all the cases the performances were higher in shrimp diet than pellet diet. It is suggested that these physiological parameters can be conveniently employed as health monitoring tools in fish culture practices. ABSTRAK Kajian ini dijalankan bagi meneliti impak perbezaan suhu dan variasi diet pada parameter hematologi dan serum biokimia pada juvenil Lutjanus malabaricus, satu spesies ikan komersial penting di Malaysia. Ikan (16-18 cm) dibahagikan kepada lapan rawatan (4 suhu × 2 diet) dan terdapat tiga replikat (5 ikan setiap replikat) bagi setiap rawatan. Ikan terdedah kepada empat suhu yang berbeza (22, 26, 30 dan 34°C) dan diberi makan pelet komersial dan diet udang semula jadi. Selepas 30 hari tempoh uji kaji, sampel darah diambil dan dipindahkan ke dalam dua tiub yang berbeza daripada setiap sampel: satu yang mengandungi EDTA sebagai agen antikoagulan dan yang lain tanpa EDTA untuk pengumpulan serum. Semua parameter hematologi dan biokimia dalam semua rawatan berada dalam julat normal. Perubahan suhu dan diet menyebabkan perubahan ketara (p <0.05) dalam jumlah panjang (TL), berat badan (BW), sel darah merah (RBC), hemoglobin (HB), hematokrit (HC), trombosit (TC) dan sel darah putih (WBC) tetapi perubahan jumlah min korpuskul (MCV), min sel hemoglobin (MCH), manakala nilai min kepekatan sel hemoglobin (MCHC) tidak signifikan (p > 0.05). Nilai yang lebih tinggi dilihat pada ikan yang diternak pada 26 dan 30°C daripada yang diternak pada 22 dan 34°C ...

Blood Performance: A New Formula for Fish Growth and Health

“…Stress causes an increase in oxygen demand in fish, which causes them to compensate with a variety of compensatory actions such as increased breathing frequency [ 35 ] or decreased synthesis of Hb ( Table S2 and see review [ 36 ]). In the present review, the decrease of Hb with many different stressors such as ammonia [ 25 , 37 ], low temperature [ 38 ], microplastics [ 39 ], pollutions [ 40 ], pathogens [ 41 , 42 ], and heavy metals [ 43 ] were observed ( Table S2 ). It seems that this decrease has been a global response to stress given that regardless of kind of stress and species, the Hb was decreased.…”

“…Unsurprisingly, the present literature search revealed that stress decreased BP and showed that this parameter can be a reliable indicator of stress ( Table S2 ). Herbicides in snow trout ( Schizothorax plagiostomus ) and common carp [ 72 , 88 , 99 ], low-temperature stress in Malabar blood snapper ( Lutjanus molabaricus ) [ 38 ], microplastics in Nile Tilapia [ 39 ], ammonia in rockfish ( Sebastes schlegelii ) [ 37 ], Aeromonas hydrophila in ningu [ 41 ], reovirus in grass carp ( Ctenopharyngodon idella ) [ 42 ] and fungus toxin in common carp [ 100 ] stimulated the decrease of BP. Furthermore, the mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and MCHC fluctuated between studies showing that they cannot be reliable enough as a biomarker of stress.…”

Effect of Stocking Density, Multispecies Probiotics, and Biofloc on Metabolic and Physiological Responses of Puntius sophore in Laboratory Conditions

“…Fish were anaesthetized with clove oil (99% purity, Talya Bitkisel Urunler Ind. Co., Ltd., Antalya, Turkey; 200 mg L −1 ) until ventilator movements stopped in order to lessen handling stress [38]. Fish were rapidly caught, and blood samples were collected from the caudal vein using a heparinized syringe and transferred to tubes that contained the anticoagulant substance EDTA.…”

Reproductive physiology, growth performances, and physiological responses of Puntius sophore: effect of probiotic, biofloc and stocking densities

“…Fish were anesthetized with clove oil (99% purity, Talya Bitkisel Urunler Ind. Co. Ltd., Antalya, Turkey; 200 mg L − 1 ) until ventilator movements stopped in order to lessen handling stress (Mazumder et al 2019). Fish were rapidly caught, and blood samples were collected from the caudal vein using a heparinized syringe and transferred to tubes that contained the anticoagulant substance EDTA.…”