Haematogical changes induced by subchronic glyphosate exposure: Ameliorative effect of zinc in Wistar rats

Abstract

The aim of this study was to determine the haematological changes induced by subchronic glyphosate exposure in Wistar rats and the ameliorative effect of zinc. Sixty adult male and female Wistar rats were used for the study. Twelve of them were used for the LD 50 which was evaluated to be 3750 mg kg-1 with clinical signs of respiratory distress, diarrhoea, rough hair coat and subsequently death observed. The remaining 48 rats were divided into six groups of four males and four females each. The agent(s) administered are as follows: Group I (DW), control, distilled water (2 ml kg-1), group II (Z), zinc (50 mg kg-1), group III (G), glyphosate (375 mg kg-1 , 10 % of the LD 50), group IV (Z + G), zinc (50 mg kg-1) and glyphosate (375 mg kg-1) while group V (GC), glyphosate (14.4 mg kg-1) and group VI (Z + GC), zinc (50 mg kg-1) and glyphosate (14.4 mg kg-1). The treatment regimens were administered orally by gavage once daily for eight weeks. At the end of the study, blood samples were collected and analysed but there were no statistical different (p>0.05) among the treatment groups. However, the haematological parameters were relatively higher in the groups treated with glyphosate alone except the lymphocyte which was relatively low in the glyphosate treated groups compared to the control group. These changes were suggestive of haematological toxicity induced by oxidative stress caused by glyphosate exposure. In conclusion, the alterations in the haematological parameters such as packed cell volume, hemoglobin concentration, total white blood cell count, neutrophils and lymphocyte counts induced by subchronic glyphosate exposure were found to be differentially ameliorated by pre-treatment with zinc.

The Potential Ameliorative Effect of Nano-Zinc and Zinc Against Copper Hepatorenal Toxicosis in Rats

“…The co-treatment of Cu intoxicated rats with N-Zn and/ or Zn corrected the anemia induced by cu by restoring RBCs count, Hb concentration, MCV, MCHC and platelets count to normal levels. This agreed with Tizhe et al (2013) who reported that Zn modulated the parameters of anemia induced by an herbicide glyphosate. This could be attributed to that Zn serves as a cofactor of many enzymes and has antioxidant effects (Powell, 2000).…”

Effect of zinc supplementation on chronic hepatorenal toxicity following oral exposure to glyphosate-based herbicide (Bushfire®) in rats

“…Group III (G1) rats were administered glyphosate orally at 14.4 mg/kg body weight (low dose, <1% LD 50 ). 24 …”

“…24 Group IV (G2) rats were administered glyphosate orally at 375 mg/kg body weight (10% LD 50 ). 24 Group V (G3) rats were administered glyphosate at 750 mg/kg body weight (20% LD 50 ). 24 Group VI (ZG1) rats were pretreated with zinc chloride (50 mg/kg) and then administered glyphosate at 14.4 mg/kg body weight 1 hour later.…”

Pancreatic function and histoarchitecture in Wistar rats following chronic exposure to Bushfire®: the mitigating role of zinc

“…Group IV (G2) received 375 mg/kg of the glyphosate-based herbicide Bushfire V R (10% of the half-maximal lethal dose [LD 50 ]). 21 Group V (G3) received 750 mg/kg Bushfire V R (20% of the LD 50 ). 21 Group VI (ZG1) was pretreated with zinc (50 mg/kg) and then administered Bushfire V R (14.4 mg/ kg) 1 hour later.…”

“…21 Group V (G3) received 750 mg/kg Bushfire V R (20% of the LD 50 ). 21 Group VI (ZG1) was pretreated with zinc (50 mg/kg) and then administered Bushfire V R (14.4 mg/ kg) 1 hour later. Group VII (ZG2) was pretreated with zinc (50 mg/kg) and then administered Bushfire V R (375 mg/kg) 1 hour later.…”