Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Baz, Farouk K. El; Salama, Abeer; Ali, Sami I.; Elgohary, Rania

doi:10.1155/2021/6631415

Cited by 13 publications

(9 citation statements)

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“…reported that feeding rats with hematococcal biomass, a rich source of AST, increased thiobarbituric acid reactive substance and the antioxidant enzymes peroxidase, catalase, and SOD in plasma and liver ( 34 ). The results of another study indicated that AST from Haematococcus pluvialis showed the best antioxidant properties and free radical scavenging in rats compared to β-carotene and lutein ( 35 ). In db/db mice, AST not only attenuated the levels of oxidative stress markers including superoxide anion and MDA but also increased SOD in the retinal tissue.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of astaxanthin and metformin combined therapy in type 2 diabetes mellitus patients: a randomized double-blind controlled clinical trial

Rad

Ahmad²,

Feizi³

et al. 2022

Res Pharma Sci

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Background and purpose: Since the critical role of oxidative stress in the pathogenesis and complications of type 2 diabetes mellitus (T2DM) has been proven, antioxidant therapy is considered an applicable strategy to control T2DM development. This study aimed at evaluating the effect of astaxanthin (AST) supplementation combined with metformin on oxidative indices and antioxidant defenses in T2DM patients. Experimental approach: In this randomized, double-blind placebo-controlled trial, 50 T2DM subjects receiving metformin were supplemented with 10 mg/day AST or placebo for 12 weeks. Malondialdehyde concentration and serum total antioxidant capacity (TAC) were assessed as oxidative indices. We also evaluated NF-E2-related factor2 (Nrf2) as the most critical transcription factor of antioxidant defense. Moreover, the activity of antioxidant enzymes, superoxide dismutase (SOD), and catalase were calculated. Findings/Results: AST supplementation-metformin combination caused a significant increase in SOD and catalase activities, as well as inducing Nrf2 protein expression compared to the placebo group. Significant changes in serum malondialdehyde and TAC between the AST group and placebo group after supplementation were not observed, although a significant increase was observed in TAC within the AST group after supplementation (32.67 ± 6.73) to before (25.86 ± 5.98). These results remained without change after adjustment for potential confounders. Conclusion and implications: Our study demonstrated that AST supplementation controlled oxidative stress through a synergistic effect with metformin and ameliorated overall antioxidant capacity by inducing Nrf2 transcription factor and activating SOD and catalase in T2DM patients. As a result, AST and metformin combination therapy can be considered beneficial in modifying oxidative stress and preventing T2DM complications.

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Section: Discussionmentioning

confidence: 99%

Antioxidant effects of astaxanthin and metformin combined therapy in type 2 diabetes mellitus patients: a randomized double-blind controlled clinical trial

Rad

Ahmad²,

Feizi³

et al. 2022

Res Pharma Sci

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“…Therefore, discovery of most effective and less toxic treatment for liver diseases have been increasingly gaining attention. Various natural products have been extensively evaluated for their hepatoprotective role against liver injury caused by different substances ( Salama et al, 2018 , Adewale et al, 2019 , Zhang et al, 2020 , El-Baz et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Shareef

Ibrahim

Alzahrani

et al. 2022

Saudi Journal of Biological Sciences

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Ethnopharmacological relevance Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been expansively investigated for its beneficial properties of cancer prevention and therapy. The goal of the research: The current study was conducted to evaluate the hepaprotective character of methanolic green tea extract and its mechanism of action contrary to thioacetamide (TAA)-produced liver fibrosis of Sprague Dawley rats. Materials and Methods Thirty rodents were equally placed in 5 clusters including normal control, TAA group as a positive control, silymarin as standard drug control, and treatment groups consisting of high dose and a low dose Camellia sinensis. Rats in experimental clusters by mouth fed with C. sinensis at 250 mg/kg or 500 mg/kg daily for 2 months. After 60 days, all rats were sacrificed. Blood specimens were gathered for liver biochemical examination. Livers of all groups were dissected out and subjected to histopathological examination through the Hematoxylin and Eosin stain, Masson trichrome, and immunohistochemistry stains (PCNA). Liver tissue homogenate was also analyzed for antioxidant activity parameters. Results Gross morphological examination showed a regular liver architecture in C. sinensis fed collections compared to the TAA sets. Histology of rat’s liver fed with C. sinensis showed an important decrease in the liver index with hepatic cells propagation, mild cellular injury, and immunostaining showed significant down-expression of proliferating cell nuclear antigen (PCNA). TAA produced liver fibrosis through a significant increase in serum alanine transferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin. Total protein and albumin also decreased in the TAA group. Moreover, the reduction of antioxidant enzyme activity including superoxide dismutase and catalase as well as the increase in malondialdehyde was detected in the TAA control group. Meanwhile, an abnormal level of liver biochemical parameters was restored closer to the normal levels in serum of the C. sinensis-fed clusters. In addition, C. sinensis fed assemblies showed elevated antioxidative enzymes activity with a reduction in malondialdehyde level comparable to the levels in silymarin-treated rats. Conclusions Green tea potentially inhibited the progression of liver cirrhosis, down -regulation of PCNA proliferation, prevented oxidation of hepatocytes, recovered SOD and CAT enzymes, condensed MDA and reduced cellular inflammation.

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“…Rats were divided into four groups ( n = 10), that is, control group (healthy rats), diseased group, quercetin treated group, and silymarin treated group. For the induction of liver fibrosis, thioacetamide (200 mg/kg) was used for 6 weeks 21 . Rats in the diseased group received intraperitoneal injections of thioacetamide twice a week for 6 weeks.…”

Section: Methodsmentioning

confidence: 99%

“…For the induction of liver fibrosis, thioacetamide (200 mg/kg) was used for 6 weeks. 21 Rats in the diseased group received intraperitoneal injections of thioacetamide twice a week for 6 weeks. Quercetin treated group received intraperitoneal injections of thioacetamide twice a week for 6 weeks, followed by oral intake of quercetin (50 mg/kg) daily for 4 weeks.…”

Section: Experimental Designmentioning

confidence: 99%

Quercetin ameliorates thioacetamide‐induced hepatic fibrosis and oxidative stress by antagonizing the Hedgehog signaling pathway

Aslam

Sheikh

Shahzad

et al. 2022

J of Cellular Biochemistry

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The Hedgehog (Hh) pathway has emerged as a potential target for effectual hepatic repair based on convincing clinical and preclinical evidence that proves its significance in regulating hepatic damage. The purpose of this study is to probe the effect of quercetin on liver fibrosis through the modulation of the Hh pathway. Healthy male Wistar rats were divided into four groups (n = 10). The control group was treated with saline, rats in the remaining three groups received twice a week intoxication with intraperitoneal injections of thioacetamide (200 mg/kg) for the induction of hepatic fibrosis for 6 weeks. After 28 days of quercetin and silymarin treatment, histological changes, serum biochemical index, antioxidant enzyme activity, key mediators of Hh pathway and inflammation were analyzed. Serological analysis showed statistically improved cholesterol, H.D.L‐Cholesterol, and L.D.L‐Cholesterol in the treatment groups. Superoxide dismutase and glutathione levels were found to be increased after the treatment with quercetin and silymarin. mRNA expression of important mediators of the Hh signaling, and inflammation including Shh, Ihh, Ptch‐1, Smo, Hhip, Gli‐3, TNF‐α, NFκ‐β, and Socs‐3 were significantly downregulated after the use of quercetin and silymarin. Quercetin also minimized the thioacetamide‐induced histopathological changes, as confirmed by a lower degree of hepatic lobule degeneration, the intralobular occurrence of inflammatory cells, and a lower degree of hepatocytic necrosis. Sudan Black B staining showed remarked lipids improvements in the treatment groups. Taken together, these findings demonstrate that quercetin could ameliorate hepatic fibrosis by antagonizing the hedgehog pathway and also suggest the hedgehog pathway as a potential therapeutic target for the treatment of liver fibrosis.

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Haematococcus pluvialis Carotenoids Enrich Fractions Ameliorate Liver Fibrosis Induced by Thioacetamide in Rats: Modulation of Metalloproteinase and Its Inhibitor

Cited by 13 publications

References 50 publications

Antioxidant effects of astaxanthin and metformin combined therapy in type 2 diabetes mellitus patients: a randomized double-blind controlled clinical trial

Antioxidant effects of astaxanthin and metformin combined therapy in type 2 diabetes mellitus patients: a randomized double-blind controlled clinical trial

Hepatoprotective effects of methanolic extract of green tea against Thioacetamide-Induced liver injury in Sprague Dawley rats

Quercetin ameliorates thioacetamide‐induced hepatic fibrosis and oxidative stress by antagonizing the Hedgehog signaling pathway

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