HACCP-based food quality control and rapid detection methods for microorganisms

Vanne, Liisa; Karwoski, Merja; Karppinen, Sirpa; Sjöberg, Anna‐Maija

doi:10.1016/s0956-7135(96)00064-3

Cited by 42 publications

(16 citation statements)

References 91 publications

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“…Factors that limit the applicability of DNA microarray for detection of pathogens are the time and effort required to isolate DNA from microorganisms, the labeling and hybridization of the DNA to the microarray, and the expensive laboratory equipment required to analyze the hybridization profile. Other rapid bacterial detection techniques have been developed using emerging analytical methods [9,10]. Mathew et al [11] developed a new method based on a chemiluminescence assay for the detection of E. coli, whereby the light emitted from a chemical reaction involving an E. coli enzyme was measured and data correlated with the E. coli density ranging from 10 5 to 10 8 cells/mL.…”

Section: Introductionmentioning

confidence: 99%

Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice

Naja

Bouvrette

Champagne

et al. 2009

Appl Biochem Biotechnol

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Section: Introductionmentioning

confidence: 99%

Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice

Naja

Bouvrette

Champagne

et al. 2009

Appl Biochem Biotechnol

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“…Reverse transcription-PCR, in particular, is a potentially valuable technique for the detection of viable bacteria (13,27); however, these techniques require the extraction of nucleic acid from samples, and PCRs are sometimes inhibited by components of food, such as lipids, proteins, and salts (30). Enzyme-linked immunosorbent assay (ELISA) is also used frequently for the detection of bacteria in food (28); however, it is often used in conjunction with culture methods and incurs the limitations of culture techniques in enumerating bacterial cells. Fluorescence in situ hybridization (FISH) is widely used and is also suitable for the specific detection of targeted bacteria phylogenetically at species, genus, and family levels (2,4), because the databases of rRNA sequences are publicly available.…”

mentioning

confidence: 99%

Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization following Formazan Reduction

Kitaguchi

Yamaguchi

Nasu

2005

Appl Environ Microbiol

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Respiring Pseudomonas spp. in milk were quantified within 6 h by fluorescence in situ hybridization (FISH) with vital staining. FISH with an oligonucleotide probe based on 16S rRNA sequences was used for the specific detection of Pseudomonas spp. at the single cell level. 5-Cyano-2,3-ditolyl tetrazolium chloride (CTC) was used to estimate bacterial respiratory activity. The numbers of respiring Pseudomonas cells as determined by FISH with CTC staining (CTC-FISH) were almost the same or higher than the numbers of CFU as determined by the conventional culture method.Conventional culture methods are commonly used for the microbiological quality assurance of food and drink. These methods are simple but generally require more than 24 h to yield reliable results. Underestimation of bacterial numbers sometimes occurs, because the cells which are viable but no longer culturable by culture methods are difficult to detect. Therefore, rapid and simple culture-independent methods are required.Several culture-independent methods are used for the detection of bacteria in food. PCR is one of the most useful techniques because of its high sensitivity. Reverse transcription-PCR, in particular, is a potentially valuable technique for the detection of viable bacteria (13, 27); however, these techniques require the extraction of nucleic acid from samples, and PCRs are sometimes inhibited by components of food, such as lipids, proteins, and salts (30). Enzyme-linked immunosorbent assay (ELISA) is also used frequently for the detection of bacteria in food (28); however, it is often used in conjunction with culture methods and incurs the limitations of culture techniques in enumerating bacterial cells. Fluorescence in situ hybridization (FISH) is widely used and is also suitable for the specific detection of targeted bacteria phylogenetically at species, genus, and family levels (2, 4), because the databases of rRNA sequences are publicly available.We attempted to enumerate Pseudomonas spp. with physiological activity in milk by combining FISH with 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) staining (CTC-FISH). Pseudomonas spp. are among the most important spoilage bacteria in milk (5, 6). They grow actively during refrigerated storage and produce enzymes, such as protease and lipase, which cause the degradation of milk compounds and reduce its shelf life. CTC is a redox dye widely used for the evaluation of bacterial respiratory activity (23,24). CTC staining can be applied to anaerobic as well as aerobic bacteria (3). CTC is reduced to red fluorescent CTC-formazan crystals in actively respiring cells. However, the crystals are easily dissolved in organic solvents used in the main processes of FISH, and CTC-stained cells are no longer visible with the conventional FISH procedure.We optimized the CTC concentration and the procedure for FISH in order to retain CTC-formazan crystals inside cells and detect bacteria with a specific rRNA sequence simultaneously. In addition, the numbers of viable Pseudomonas cells determined by CTC-FI...

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“…The ATP measurement for microbial cells with the firefly luciferin-luciferase method is rapid and simple (17,31). It takes less than 1 min to measure a sample with the ATP analyzer that we used.…”

Section: Resultsmentioning

confidence: 99%

Modeling Surface Growth of Escherichia coli on Agar Plates

Fujikawa

Morozumi

2005

Appl Environ Microbiol

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Surface growth curves at various initial cell numbers were also sigmoidal and converged to the same maximum cell numbers at the stationary phase. Surface growth curves at various nutrient levels were also sigmoidal. The maximum cell number and the rate of growth were lower as the nutrient level decreased. The surface growth curve was the same as that in a liquid, except for the large curvature at the deceleration period. These curves were also well described with model III. The pattern of increase in the ATP content of cells grown on a surface was sigmoidal, similar to that for cell growth. We discovered several characteristics of the surface growth of bacterial cells under various growth conditions and examined the applicability of our model to describe these growth curves.

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HACCP-based food quality control and rapid detection methods for microorganisms

Cited by 42 publications

References 91 publications

Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice

Activation of Nanoparticles by Biosorption for E. coli Detection in Milk and Apple Juice

Enumeration of Respiring Pseudomonas spp. in Milk within 6 Hours by Fluorescence In Situ Hybridization following Formazan Reduction

Modeling Surface Growth of Escherichia coli on Agar Plates

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