Habitual long-distance running does not enhance urinary excretion of 8-hydroxydeoxyguanosine

Pilger, Alexander; Germadnik, D.; Formanek, D.; Zwick, Hartmut; Winkler, Nurit; Rüdiger, H. W.

doi:10.1007/s004210050190

Cited by 23 publications

(9 citation statements)

References 11 publications

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“…Increased urinary 8-OHdG levels were observed in 23 healthy males in response to a vigorous physical training programme (about 10 h of exercise for 30 d) [49] and in male long-distance runners throughout a training period for 8 d compared to a sedentary period [50]. However, in a longitudinal study no differences in urinary excretion of 8-OHdG between a group of long-distance runners and a sedentary control group were observed [51]. Our data showed a decrease (∼25%) in urinary 8-OH-dG excretion in both MOD and HIDT groups.…”

Section: Discussionmentioning

confidence: 87%

Time-Course Changes of Oxidative Stress Response to High-Intensity Discontinuous Training versus Moderate-Intensity Continuous Training in Masters Runners

et al. 2014

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Beneficial systemic effects of regular physical exercise have been demonstrated to reduce risks of a number of age-related disorders. Antioxidant capacity adaptations are amongst these fundamental changes in response to exercise training. However, it has been claimed that acute physical exercise performed at high intensity (>60% of maximal oxygen uptake) may result in oxidative stress, due to reactive oxygen species being generated excessively by enhanced oxygen consumption. The aim of this study was to evaluate the effect of high-intensity discontinuous training (HIDT), characterized by repeated variations of intensity and changes of redox potential, on oxidative damage. Twenty long-distance masters runners (age 47.8±7.8 yr) on the basis of the individual values of gas exchange threshold were assigned to a different 8-weeks training program: continuous moderate-intensity training (MOD, n = 10) or HIDT (n = 10). In both groups before (PRE) and after (POST) training we examined the following oxidative damage markers: thiobarbituric acid reactive substances (TBARS) as marker of lipid peroxidation; protein carbonyls (PC) as marker of protein oxidation; 8-hydroxy-2-deoxy-guanosine (8-OH-dG) as a biomarker of DNA base modifications; and total antioxidant capacity (TAC) as indicator of the overall antioxidant system. Training induced a significant (p<0.05) decrease in resting plasma TBARS concentration in both MOD (7.53±0.30 and 6.46±0.27 µM, PRE and POST respectively) and HIDT (7.21±0.32 and 5.85±0.46 µM, PRE and POST respectively). Resting urinary 8-OH-dG levels were significantly decreased in both MOD (5.50±0.66 and 4.16±0.40 ng mg−1creatinine, PRE and POST respectively) and HIDT (4.52±0.50 and 3.18±0.34 ng mg−1creatinine, PRE and POST respectively). Training both in MOD and HIDT did not significantly modify plasma levels of PC. Resting plasma TAC was reduced in MOD while no significant changes were observed in HIDT. In conclusion, these results suggest that in masters runners high-intensity discontinuous does not cause higher level of exercise-induced oxidative stress than continuous moderate-intensity training, inducing similar beneficial effects on redox homeostasis.

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Section: Discussionmentioning

confidence: 87%

Time-Course Changes of Oxidative Stress Response to High-Intensity Discontinuous Training versus Moderate-Intensity Continuous Training in Masters Runners

et al. 2014

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“…Exercise has been shown to lower concentration of oxidized DNA in skeletal muscle without affecting TBARS and protein carbonyls [35]. These studies may indicate that oxidative stress induces antioxidant systems to cope with ROS [36].…”

Section: Discussionmentioning

confidence: 91%

Organ Specific Changes in Markers of Oxidative Stress in Choline, Carnitine and Caffeine Supplemented and Exercised Rats

Sachan¹,

Johnsen²,

Hongu³

2012

TONUTRJ

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Objective: of this study was to determine changes in oxidative indices in tissues of exercised rats fed diet fortified with choline, carnitine and caffeine; and relate to those reported in humans.Methods: 7-week old male Sprague-Dawley rats were studied for five weeks. Diets were supplemented with choline, carnitine and caffeine and rats were either exercised or not during the last 2 weeks of the dietary regimen. Tissue samples were collected at the end of the period and analyzed for the concentrations of TBARS, hydroperoxides and protein carbonyls. Results:The changes modulated by the supplement as well as exercise regimens in the oxidative stress markers were tissue specific. The changes in serum TBARS of rats were unlike those in women under similar conditions of treatment we reported earlier. Conclusions:The effects of choline, carnitine and caffeine supplements on the indices of oxidative stress were tissue specific and were not always correlated with serum TBARS. The changes in serum TBARS in rats were contrary to those reported in humans under similar conditions of treatment.

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“…When long-distance runners were compared with nonactive controls, no differences in the urinary 8-OHdG levels were found at rest; however, no data on the subjects' training statuses were shown. 32 In contrast, decreased levels of plasma 8-OHdG (measured with ELISA) was found immediately after a 10-km run (1 ± 0.2-h duration), which returned to baseline 24 hours after exercise. 33 Urinary 8-OHdG was increased in long-distance runners during an eight-day running camp, with an average running distance of 30 ± 6 km/day.…”

Section: Noncompetitive Field Studies With Endurance Exercise and Permentioning

confidence: 85%

“…No change in urinary 8‐OHdG was assessed, either three days before, or three days after, the test. When long‐distance runners were compared with nonactive controls, no differences in the urinary 8‐OHdG levels were found at rest; however, no data on the subjects’ training statuses were shown 32 . In contrast, decreased levels of plasma 8‐OHdG (measured with ELISA) was found immediately after a 10‐km run (1 ± 0.2‐h duration), which returned to baseline 24 hours after exercise 33 …”

Section: Noncompetitive Field Studies With Endurance Exercise and Permentioning

confidence: 97%

Impact of endurance and ultraendurance exercise on DNA damage

Wagner

Reichhold

Neubauer

2011

Annals of the New York Academy of Sciences

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Regular moderate physical activity reduces the risk of several noncommunicable diseases. At the same time, evidence exists for oxidative stress resulting from acute and strenuous exercise by enhanced formation of reactive oxygen and nitrogen species, which may lead to oxidatively modified lipids, proteins, and possibly negative effects on DNA stability. The limited data on ultraendurance events such as an Ironman triathlon show no persistent DNA damage after the events. However, when considering the effects of endurance exercise comparable to a (half) marathon or a short triathlon distance, no clear conclusions could be drawn. In order to clarify which components of exercise participation, such as duration, intensity, frequency, or training status of the subjects, have an impact on DNA stability, more information is clearly needed that combines the measurement of DNA damage, gene expression, and DNA repair mechanisms before, during, and after exercise of differing intensities and durations.Keywords: ultraendurance exercise; endurance exercise; physical activity; DNA damage; training status Oxidative stress-induced DNA damage and insufficient DNA repair have been discussed to play a significant role in processes that lead to chronic diseases, such as cancer, diabetes, and arteriosclerosis. 1It is well documented that regular moderate physical activity is associated with various health benefits, including decreased risk of the mentioned diseases; [2][3][4] however, acute, but not regular, strenuous exercise may induce oxidative stress via enhanced formation of reactive oxygen (ROS) and nitrogen species (RNS). 5,6 The potential mechanisms involved in this formation are increased oxygen consumption, autoxidation of catecholamines, activation of inflammatory cells due to muscle tissue damage, and ischemia and/or hypoxia/reoxygenation damage. 7ROS formation results in oxidative modifications of lipids, proteins, and nucleic acids. [7][8][9] Since oxidative modifications of DNA can lead to mutations 10 and exceptionally high volumes of exercise are also associated with a substantial oxidative stress, concerns have arisen about the health effects of competing in endurance and ultraendurance exercise events, particularly when participants are not optimally trained.11 Based on the U -shaped relationship between exercise and health in general, much has to be learned on exercise of different durations, intensities, and types on oxidative stress responses and DNA stability. The current work of our group is focusing on the effects of ultraendurance exercise on oxidative stress and DNA stability. Methods commonly used to evaluate DNA damage linked to exerciseComprehensive reviews have been published on the various methods that quantify genotoxicity in human studies 12 and therefore are not the subject of this paper.Very briefly, the most commonly applied methodologies to describe changes in DNA stability during or after physical activity are the micronucleus (MN) assay; its more developed test, the cytokinesis-block micronucle...

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Habitual long-distance running does not enhance urinary excretion of 8-hydroxydeoxyguanosine

Cited by 23 publications

References 11 publications

Time-Course Changes of Oxidative Stress Response to High-Intensity Discontinuous Training versus Moderate-Intensity Continuous Training in Masters Runners

Time-Course Changes of Oxidative Stress Response to High-Intensity Discontinuous Training versus Moderate-Intensity Continuous Training in Masters Runners

Organ Specific Changes in Markers of Oxidative Stress in Choline, Carnitine and Caffeine Supplemented and Exercised Rats

Impact of endurance and ultraendurance exercise on DNA damage

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