Hábito alimentar de Triatoma vitticeps no Estado do Rio de Janeiro, Brasil

Gonçalves, Teresa Cristina Monte; Rocha, Dayse da Silva; Cunha, Rodolfo Armando da

doi:10.1590/s0034-89102000000400006

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…In contrast to findings from other areas ( Dias et al 1989 , Gonçalves et al 1998 , 2000 , Leite et al 2010 ), more males than females of T. vitticeps were captured in Diamantina . This discrepancy may be due to population differences driven by environmental characteristics that may influence insect dispersal.…”

Section: Discussioncontrasting

confidence: 93%

Spatial distribution of triatomines in domiciles of an urban area of the Brazilian Southeast Region

Dias

Queiroz

Martins

et al. 2016

Mem. Inst. Oswaldo Cruz

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Reports of triatomine infestation in urban areas have increased. We analysed the spatial distribution of infestation by triatomines in the urban area of Diamantina, in the state of Minas Gerais, Brazil. Triatomines were obtained by community-based entomological surveillance. Spatial patterns of infestation were analysed by Ripley’s K function and Kernel density estimator. Normalised difference vegetation index (NDVI) and land cover derived from satellite imagery were compared between infested and uninfested areas. A total of 140 adults of four species were captured (100 Triatoma vitticeps, 25Panstrongylus geniculatus, 8 Panstrongylus megistus, and 7 Triatoma arthurneivai specimens). In total, 87.9% were captured within domiciles. Infection by trypanosomes was observed in 19.6% of 107 examined insects. The spatial distributions ofT. vitticeps, P. geniculatus, T. arthurneivai, and trypanosome-positive triatomines were clustered, occurring mainly in peripheral areas. NDVI values were statistically higher in areas infested by T. vitticeps and P. geniculatus. Buildings infested by these species were located closer to open fields, whereas infestations of P. megistus andT. arthurneivai were closer to bare soil. Human occupation and modification of natural areas may be involved in triatomine invasion, exposing the population to these vectors.

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Section: Discussioncontrasting

confidence: 93%

Spatial distribution of triatomines in domiciles of an urban area of the Brazilian Southeast Region

Dias

Queiroz

Martins

et al. 2016

Mem. Inst. Oswaldo Cruz

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“…In the posterior midgut region, digestive cells had vesicles with various aspects, and they might be disperse throughout cytoplasm, as well as being released among microvilli (Figs. 16,17) and lipid droplets and hemozoin granules that are characterized by strong density to electrons (Fig. 19).…”

Section: Resultsmentioning

confidence: 99%

“…To date, available studies about the morphology of triatomines midgut are describing Rhodnius prolixus and Triatoma infestans, because of their importance as vectors for Chagas' disease. However, recent findings have warned about the risk that domiciliating some wild species such as T. vitticeps represents for public health [16,17].…”

Section: Introductionmentioning

confidence: 99%

Digestive cells in the midgut of Triatoma vitticeps (Stal, 1859) in different starvation periods

Rocha

Neves

Zanúncio

et al. 2010

Comptes Rendus. Biologies

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Triatoma vitticeps (Stal, 1859) is a hematophagous Hemiptera that, although being considered wild, can be found in households, being a potential Chagas' disease vector. This work describes the histology and ultrastructure of the midgut of T. vitticeps under different starvation periods. Fifteen adults of both sexes starved for 3, 7, 20 and 25 days were studied. In general, digestive cells had apical microvilli, basal plasma membrane infoldings and central nucleus. The perimicrovillar membrane was found in all insects examined. Digestive cells of anterior midgut had lipid droplets, glycogen granules, developed basal labyrinth associated with mitochondria suggesting their role in nutrient storage and in fluid and ion transport. The cells of median and posterior regions of the midgut were rich in rough endoplasmic reticulum, lysosomes, vesicles and granules with different electron-densities. Moreover, cells of the posterior portion of the midgut had hemozoyn granules and mitochondria in the apical cytoplasm close to microvilli, suggesting their role in blood digestion and active nutrient absorption. The midgut of T. vitticeps showed differences in digestive cells associated with the time after feeding, and the increase of vesicles amount in long starvation periods, which suggests enzyme storage, which is readily used after a blood meal.

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“…In spite of the fact that triatomine vectors digest blood meals slowly (up to 10 weeks) [ 14 , 16 , 24 , 29 ], DNA-based methods for identifying blood meal sources fail in upwards of 50% of Chagas insect vectors [ 23 , 24 , 33 – 35 , 37 , 38 , 63 , 64 ]. In this regard, hemoglobin is a particularly stable protein and appears to last longer than DNA signatures [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Chagas disease vector blood meal sources identified by protein mass spectrometry

et al. 2017

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Chagas disease is a complex vector borne parasitic disease involving blood feeding Triatominae (Hemiptera: Reduviidae) insects, also known as kissing bugs, and the vertebrates they feed on. This disease has tremendous impacts on millions of people and is a global health problem. The etiological agent of Chagas disease, Trypanosoma cruzi (Kinetoplastea: Trypanosomatida: Trypanosomatidae), is deposited on the mammalian host in the insect’s feces during a blood meal, and enters the host’s blood stream through mucous membranes or a break in the skin. Identifying the blood meal sources of triatomine vectors is critical in understanding Chagas disease transmission dynamics, can lead to identification of other vertebrates important in the transmission cycle, and aids management decisions. The latter is particularly important as there is little in the way of effective therapeutics for Chagas disease. Several techniques, mostly DNA-based, are available for blood meal identification. However, further methods are needed, particularly when sample conditions lead to low-quality DNA or to assess the risk of human cross-contamination. We demonstrate a proteomics-based approach, using liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify host-specific hemoglobin peptides for blood meal identification in mouse blood control samples and apply LC-MS/MS for the first time to Triatoma dimidiata insect vectors, tracing blood sources to species. In contrast to most proteins, hemoglobin, stabilized by iron, is incredibly stable even being preserved through geologic time. We compared blood stored with and without an anticoagulant and examined field-collected insect specimens stored in suboptimal conditions such as at room temperature for long periods of time. To our knowledge, this is the first study using LC-MS/MS on field-collected arthropod disease vectors to identify blood meal composition, and where blood meal identification was confirmed with more traditional DNA-based methods. We also demonstrate the potential of synthetic peptide standards to estimate relative amounts of hemoglobin acquired when insects feed on multiple blood sources. These LC-MS/MS methods can contribute to developing Ecohealth control strategies for Chagas disease transmission and can be applied to other arthropod disease vectors.

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Hábito alimentar de Triatoma vitticeps no Estado do Rio de Janeiro, Brasil

Cited by 9 publications

References 7 publications

Spatial distribution of triatomines in domiciles of an urban area of the Brazilian Southeast Region

Spatial distribution of triatomines in domiciles of an urban area of the Brazilian Southeast Region

Digestive cells in the midgut of Triatoma vitticeps (Stal, 1859) in different starvation periods

Chagas disease vector blood meal sources identified by protein mass spectrometry

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