H2S signaling and extracellular matrix remodeling in cardiovascular diseases: A tale of tense relationship

Zhu, Jiechun; Yang, Guangdong

doi:10.1016/j.niox.2021.08.004

Cited by 5 publications

(3 citation statements)

References 168 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Stiffness and adhesive cues of ECM provide cancer cells with mechanical signals that are transduced within the cells primarily by cytoskeleton anchored proteins and affect gene expression by supporting programs that serve cells to respond to such stimuli, by altering cell architecture, fostering motility and overcoming stresses induced by the mechanical pressure [ 28 ]. Aberrant ECM remodeling is initiated by different types of stress stimuli, including oxidative stress [ 29 ].Deposition of asbestos fibers to the lungs causes the chronic release of reactive species and the production of free radicals. These highly dynamics elements may cause massive molecular instability and concur to reshape ECM during MPM progression [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy

et al. 2023

View full text Add to dashboard Cite

Background Malignant Pleural Mesothelioma (MPM) is a dreadful disease escaping the classical genetic model of cancer evolution and characterized by wide heterogeneity and transcriptional plasticity. Clinical evolution of MPM is marked by a progressive transdifferentiation that converts well differentiated epithelioid (E) cells into undifferentiated and pleomorphic sarcomatoid (S) phenotypes. Catching the way this transition takes place is necessary to understand how MPM develops and progresses and it is mandatory to improve patients’ management and life expectancy. Bulk transcriptomic approaches, while providing a significant overview, failed to resolve the timing of this evolution and to identify the hierarchy of molecular events through which this transition takes place. Methods We applied a spatially resolved, high-dimensional transcriptomic approach to study MPM morphological evolution. 139 regions across 8 biphasic MPMs (B-MPMs) were profiled using the GeoMx™Digital Spatial Profiler to reconstruct the positional context of transcriptional activities and the spatial topology of MPM cells interactions. Validation was conducted on an independent large cohort of 84 MPMs by targeted digital barcoding analysis. Results Our results demonstrated the existence of a complex circular ecosystem in which, within a strong asbestos-driven inflammatory environment, MPM and immune cells affect each other to support S-transdifferentiation. We also showed that TGFB1 polarized M2-Tumor Associated Macrophages foster immune evasion and that TGFB1 expression correlates with reduced survival probability. Conclusions Besides providing crucial insights into the multidimensional interactions governing MPM clinical evolution, these results open new perspectives to improve the use of immunotherapy in this disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It is often suggested that H 2 S diffuses through the extracellular membrane, but at pH 7.4, the large majority of sulfide is ionized as HS − or S 2− [ 39 ], these ions should not diffuse through the cell membrane at the rates observed. Many reports implicate H 2 S as a paracrine signaling agent at an intracellular level (i.e., cysteine persulfidation) as well as extracellular level (extracellular matrix maintenance and remodeling) [ 92 , 93 , 94 ]. However, no intramembrane channel responsible for transporting ionized H 2 S has been identified yet.…”

Section: Discussionmentioning

confidence: 99%

Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H2S in Human Primary Vascular Cell Culture

et al. 2021

View full text Add to dashboard Cite

Hydrogen sulfide (H2S) is an endogenous signaling molecule which is important for cardiovascular health, but its mechanism of action remains poorly understood. Here, we report measurements of H2S as well as its oxidized metabolites, termed small oxoacids of sulfur (SOS = HSOH and HOSOH), in four human primary vascular cell lines: smooth muscle and endothelial cells derived from both human arterial and coronary tissues. We use a methodology that targets small molecular weight sulfur species; mass spectrometric analysis allows for species quantification to report cellular concentrations based on an H2S calibration curve. The production of H2S and SOS is orders of magnitude higher in smooth muscle (nanomolar) as compared to endothelial cell lines (picomolar). In all the primary lines measured, the distributions of these three species were HOSOH >H2S > HSOH, with much higher SOS than seen previously in non-vascular cell lines. H2S and SOS were effluxed from smooth muscle cells in higher concentrations than endothelial cells. Aortic smooth muscle cells were used to examine changes under hypoxic growth conditions. Hypoxia caused notable increases in HSOH and ROS, which we attribute to enhanced sulfide quinone oxidase activity that results in reverse electron transport.

show abstract

“…[124] H 2 S not only affects the synthesis of some ECM components (collagen, elastin, and fibronectin), but also regulates the expression and activity of several proteins in ECM. [125] In addition, it has been proved that H 2 S can open the K ATP channel of smooth muscle cells for vasorelaxation, [30] and protect the heart by protecting mitochondrial function, regulating macrophage function, [126] inhibiting oxidative stress, [127] and alleviating inflammation. [128] In recent years, H 2 S delivery technology has made continuous breakthroughs in the treatment of related cardiovascular diseases.…”

Section: Cardiovascular Diseasesmentioning

confidence: 99%

Current Status and Future Prospects of Hydrogen Sulfide Donor‐Based Delivery Systems

Chen

2023

Advanced Therapeutics

View full text Add to dashboard Cite

Nowadays, hydrogen sulfide (H2S) is regarded as an endogenous gaseous signaling molecule in mammals. As a gas transmitter, it induces important biological effects in many physiological and pathological processes, and possesses great therapeutic potentials. However, this bioactive gas has concentration‐related toxicity, rapid evaporation, and high reactivity, making it inconvenient to use as a therapeutic agent. As an exogenous source of H2S, various H2S donors are developed to release H2S after triggering the response under specific conditions, so as to supplement the level of H2S in the body. Meanwhile, to enhance the therapeutic efficacy and reduce the possible toxicities of the H2S donors, various new materials or methods for H2S delivery are studied, realizing sustained/controlled release profiles as well as achieving triggered, long‐term, local or targeted delivery approaches for the gas. This review summarizes the representative H2S donors and the latest progress of hydrogen sulfide donor‐based delivery systems, and the applications of such delivery systems in a series of target diseases that are suitable and expected to be treated by H2S, in order to provide reference for the design of secure and efficient H2S donor drug delivery system. In addition, the future of this field is also prospected.

show abstract

H2S signaling and extracellular matrix remodeling in cardiovascular diseases: A tale of tense relationship

Cited by 5 publications

References 168 publications

Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy

Spatially resolved, high-dimensional transcriptomics sorts out the evolution of biphasic malignant pleural mesothelioma: new paradigms for immunotherapy

Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H2S in Human Primary Vascular Cell Culture

Current Status and Future Prospects of Hydrogen Sulfide Donor‐Based Delivery Systems

Contact Info

Product

Resources

About