In order to remove hydrogen sulfide from petroleum refinery, a fixedbed bioreactor was constructed by the immobilized Thiobacillus ferrooxidans (T.f ) with H-3 carrier. In the state of bioreactor operation, a maximum Fe 2C oxidation of 7.24 g/L.h was attained with the aeration of 300 L/h, the dilution rate of 0.6 h 1 , the pH of 2.0, and the temperature of 30 ı C. And the complex compound forming on the surface of the carrier in the operation of bioreactor was identified as jarosite by chemical analysis and X-ray diffraction analysis. In the fixed-bed bioreactor, the removal of hydrogen sulfide was higher than 99.12%, and the content of H 2 S in the exhaust was less than 10 g/g.