H2O2 pretreated rice seedlings specifically reduces arsenate not arsenite: difference in nutrient uptake and antioxidant defense response in a contrasting pair of rice cultivars

Abstract

The study investigated the reduction in metalloid uptake at equimolar concentrations (~53.3 μM) of As(III) and As(V) in contrasting pair of rice seedlings by pretreating with H 2 O 2 (1.0 μM) and SA (1.0 mM). Results obtained from the contrasting pair (arsenic tolerant vs. sensitive) of rice seedlings (cv. Pant Dhan 11 and MTU 7029, respectively) shows that pretreatment of H 2 O 2 and H 2 O 2 + SA reduces As(V) uptake significantly in both the cultivars, while no reduction in the As(III) uptake. The higher growth inhibition, higher H 2 O 2 and TBARS content in sensitive cultivar against As(III) and As(V) treatments along with higher As accumulation (~1.2 mg g −1 dw) than in cv. P11, unravels the fundamental difference in the response between the sensitive and tolerant cultivar. In the H 2 O 2 pretreated plants, the translocation of As increased in tolerant cultivar against AsIII, whereas, it decreased in sensitive cultivar both against AsIII and AsV. In both the cultivars translocation of Mn increased in the H 2 O 2 pretreated plants against As(III), whereas, the translocation of Cu increased against As(V). In tolerant cultivar the translocation of Fe increased against As(V) with H 2 O 2 pretreatment whereas, it decreased in the sensitive cultivar. In both the cultivars, Zn translocation increased against As(III) and decreased against As(V). The higher level of H 2 O 2 and SOD (EC 1.15.1.1) activity in sensitive cultivar whereas, higher, APX (EC 1.11.1.11), GR (EC 1.6.4.2) and GST (EC 1.6.4.2) activity in tolerant cultivar, also demonstrated the differential anti-oxidative defence responses between the contrasting rice cultivars.

Arsenic induced modulation of antioxidative defense system and brassinosteroids in Brassica juncea L.

“…The results obtained are in concordance with studies on B. juncea which are exposed to different concentrations of As and showed enhancement in stress marker parameters like MDA and proline (Khan et al, 2009;Ahmad and Gupta, 2013). Similarly higher MDA content was noticed in rice genotypes under As stress (Dave et al, 2013;Mallick et al, 2014).…”

“…The up-regulation of antioxidative defense system in rice seedlings was observed under As stress by Shri et al, (2009). Similar results were obtained in rice plants where enhanced antioxidative potential was noticed with As stress (Dave et al, 2013, Mallick et al, 2014.…”

Bioaccumulation of Potentially Toxic Elements in Cereal and Legume Crops: A Review

“…Triticum aestivum, Sorghum bicolor, Hordeum vulgare Inhibition of germination, reduce root and shoot growth [148] As(III) Nati mansuri Reduction in root shoot fresh and dry weight, decreased total chlorophyll contents and protein contents [149] As(III), As(V) Triticum aestivum L. Marked anomalies in anatomical features, reduction in pigment composition, increased [150] As(III) Phaseolus vulgaris RO and severe perturbations in antioxidant enzyme activities [66] As(V) Oryza sativa Induced oxidative stress measured regarding malondialdehyde (MDA), root oxidizability and H 2 O 2 content [151] Cd Cd(II) Cicer arietinum L. Leaf rotting and chlorosis of foliar parts decreased activities of antioxidants [152] Oryza sativa L. Reduces the nutrient translocation and absorption, and poor RNA synthesis and less ribonuclease activity and inhibition of respiration [153] Glycine max L. Browning of root hairs and tips, reddish-brown necrosis on young leaves, root and shoot growth reduction, inhibition of photosynthesis and reduction in the transpiration due to fewer stomata [154] Hordeum vulgare L. The germination and growth of roots were severely affected by the treatment of Cd @ 10 À2 mol/L [155] Triticum aestivum L. Disruption of photosynthetic apparatus, inhibit the photosynthesis, reduction in the chlorophyll content [156] Zea mays L. Inhibits the functions of chlorophyll, retard the photosynthesis and results in chlorosis [157] Pisum sativum L. Inhibits the functions of chlorophyll, retard the photosynthesis and results in chlorosis [157] Vigna radiate L. Inhibits the photosynthesis and results in chlorosis [158] Cajanus cajan L. Brown margin of leaves, chlorosis, necrosis, brown stunted roots, reddish veins, and petioles, purple stems [159] Cr Cr(VI) Vigna mungo L. Chromosomal aberrations [160] Triticum aestivum L. Reduction in the total chlorophyll content and impair the photosynthetic functions [46] Zea mays L. Lipid peroxidation, ROS production, disruption of photosynthetic apparatus, inhibit the photosynthesis, reduce the protein content [161] Pisum sativum L. ROS production, root growth inhibition [162] Vacia faba L. Reduction in the mitotic activity of root meristems [160] Cyamopsis tetragonoloba L. Adversely affect the nitrogen metabolism by inhibiting the activity of enzymes and photosynthetic apparatus [163] Co Co(VI) Cicer arietinum Poor seed germination and plant growth, poor nodulation, reduction in chlorophyll [164] Vigna radiata Decrease in antioxidant enzyme activities; reduction in pla...…”