H2O2 plays an important role in the lifestyle of Colletotrichum gloeosporioides during interaction with cowpea [Vigna unguiculata (L.) Walp.]

Abstract

Plant-fungus interactions usually generate H(2)O(2) in the infected plant tissue. H(2)O(2) has a direct antimicrobial effect and is involved in the cross-linking of cell walls, signaling, induction of gene expression, hypersensitive cell death and induced systemic acquired resistance. This has raised the hypothesis that H(2)O(2) manipulation by pharmacological compounds could alter the lifestyle of Colletotrichum gloeosporioides during interaction with the BR-3-Tracuateua cowpea genotype. The primary leaves of cowpea were excised, infiltrated with salicylic acid (SA), glucose oxidase + glucose (GO/G), catalase (CAT) or diphenyliodonium chloride (DPI), followed by spore inoculation on the adaxial leaf surface. SA or GO/G-treated plantlets showed increased H(2)O(2) accumulation and lipid peroxidation. The fungus used a subcuticular, intramural necrotrophic strategy, and developed secondary hyphae associated with the quick spread and rapid killing of host cells. However, CAT or DPI-treated leaves exhibited decreased H(2)O(2) concentration and lipid peroxidation and the fungus developed intracellular hemibiotrophic infection with vesicles, in addition to primary and secondary hyphal formation. These results suggest that H(2)O(2) plays an important role in the cowpea (C. gloeosporioides) pathosystem given that it affected fungal lifestyle during interaction.

The Loricrin-Like Protein (LLP) of Phytophthora infestans Is Required for Oospore Formation and Plant Infection

“…Impala) were infiltrated with 0.5 μM DPI (Sigma-Aldrich, St. Louis, MO, USA) in 0.05% DMSO using a disposable, plastic needleless syringe. The leaves that were infiltrated with 0.05% DMSO served as controls (Eloy et al, 2015 ).…”

Early production of reactive oxygen species coupled with an efficient antioxidant system play a role in potato resistance to late blight

“…The role of ROS in the infection process of hemibiotrophic pathogens, such as P. infestans remains controversial (Shetty et al 2007;Henriquez et al 2012;Thomazella et al 2012;Eloy et al 2015). The present study focused on potato biochemical defense responses induced during the early biotrophic and the late necrotrophic stages of P. infestans on resistant and susceptible cultivars.…”

“…During the plant-pathogen interaction, the generated ROS may play a dual role; its production in cells is highly effective against biotrophic pathogens, whereas high accumulation of H 2 O 2 has been reported to help the colonization process of plant tissues by necrotrophic pathogens (Noorbakhsh and Taheri 2016). For the hemibiotrophic pathogens, the role of H 2 O 2 in the infectious process is still poorly understood (Shetty et al 2007;Henriquez et al 2012;Thomazella et al 2012;Eloy et al 2015). In-depth studies are necessary to fully elucidate the balance between the antioxidant system and the concentration of ROS during the infection process of P. infestans.…”

Host-induced silencing of theColletotrichum gloeosporioides conidial morphology 1gene (CgCOM1) confers resistance against Anthracnose disease in chilli and tomato

“…Similar type of infection was also observed in guava (Moraes et al, 2013). However, in cowpea leaves, it was reported that C. gloeosporioides attempt to gain entry into leaf tissues through stomatal openings (Eloy et al , 2015). Under in vitro culture conditions on PDA medium, C. gloeosporioides typically exhibited growth stages like germination of conidia after 3 hpi and appressoria formation after 36 hpi; but never displayed the development of primary and secondary hyphae under these conditions.…”