H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and differentiation

Shi, Guangming; Ren, Lingfei; Wang, Lin-zhi; Lin, Hongyu; Wang, Sha-bin; Tong, Yongqing

doi:10.1016/j.tripleo.2009.05.033

Cited by 41 publications

(33 citation statements)

References 31 publications

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“…[4][5][6][7] Among these methods, chemical treatment is a relatively simple and effective technique and has been widely investigated. [8][9][10][11] In previous studies, a type of nanorod structure with a phase composition of anatase and rutile has been fabricated on a titanium surface using different chemical processes. [12][13][14] However, biofunctionalization of the nanorod structure has not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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Background and methods:Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin β1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results:The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion: This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo.

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Section: Introductionmentioning

confidence: 99%

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Huang³

et al. 2013

IJN

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“…14 Chemical treatment is a simple and effective approach and has been widely used in recent years. 15 For example, hydrogen peroxide (H 2 O 2 ) has been used to treat titanium implants to promote the deposition of apatite. 16 Additionally, other studies have shown that several nanoscale structures can be produced when the titanium surface is treated with H 2 O 2 alone or synergistically under different conditions.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Zhang¹,

Li²,

Liu³

et al. 2012

IJN

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Background:The topography of an implant surface can serve as a powerful signaling cue for attached cells and can enhance the quality of osseointegration. A series of improved implant surfaces functionalized with nanoscale structures have been fabricated using various methods. Methods: In this study, using an H 2 O 2 process, we fabricated two size-controllable sawtooth-like nanostructures with different dimensions on a titanium surface. The effects of the two nanosawtooth structures on rat bone marrow mesenchymal stem cells (BMMSCs) were evaluated without the addition of osteoinductive chemical factors. Results: These new surface modifications did not adversely affect cell viability, and rat BMMSCs demonstrated a greater increase in proliferation ability on the surfaces of the nanosawtooth structures than on a control plate. Furthermore, upregulated expression of osteogenicrelated genes and proteins indicated that the nano-sawtooth structures promote osteoblastic differentiation of rat BMMSCs. Importantly, the large nano-sawtooth structure resulted in the greatest cell responses, including increased adhesion, proliferation, and differentiation. Conclusion: The enhanced adhesion, proliferation, and osteogenic differentiation abilities of rat BMMSCs on the nano-sawtooth structures suggest the potential to induce improvements in bonetitanium integration in vivo. Our study reveals the key role played by the nano-sawtooth structures on a titanium surface for the fate of rat BMMSCs and provides insights into the study of stem cellnanostructure relationships and the related design of improved biomedical implant surfaces.

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“…To test the ability of K1 to deliver hydrophobic drugs, 4.07 mg of K-G∧C were dissolved in deuterated methanol (CD 3 1 H NMR spectra were acquired at room temperature using a single pulse excitation with a 45° flip angle of 3.6 µ seconds and an acquisition time of 1.7 seconds. The repetition time was 1.0 seconds.…”

Section: H and Dosy Nmr Studiesmentioning

confidence: 99%

“…1 A variety of materials have been investigated as bone replacements, such as conventional titanium and its alloys, micron grain sized ceramics (eg, hydroxylapatite or HA) and nanostructured hydrogels (eg, poly-lactic-co-glycolic acid or PLGA). [2][3][4][5] However, current conventional implants suffer from limited lifetimes, often failing before the end of a patient's natural life expectancy. 6,7 For example, today's orthopedic implants usually have insufficient initial bone growth on their surface and/or poor maintenance of healthy juxtaposed bone over long periods of time.…”

Section: Introductionmentioning

confidence: 99%

Self-assembled rosette nanotubes encapsulate and slowly release dexamethasone

Chen¹,

Song²,

Yan³

et al. 2011

IJN

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Rosette nanotubes (RNTs) are novel, self-assembled, biomimetic, synthetic drug delivery materials suitable for numerous medical applications. Because of their amphiphilic character and hollow architecture, RNTs can be used to encapsulate and deliver hydrophobic drugs otherwise difficult to deliver in biological systems. Another advantage of using RNTs for drug delivery is their biocompatibility, low cytotoxicity, and their ability to engender a favorable, biologically-inspired environment for cell adhesion and growth. In this study, a method to incorporate dexamethasone (DEX, an inflammatory and a bone growth promoting steroid) into RNTs was developed. The drug-loaded RNTs were characterized using diffusion ordered nuclear magnetic resonance spectroscopy (DOSY NMR) and UV-Vis spectroscopy. Results showed for the first time that DEX can be easily and quickly encapsulated into RNTs and released to promote osteoblast (bone-forming cell) functions over long periods of time. As a result, RNTs are presented as a novel material for the targeted delivery of hydrophobic drugs otherwise difficult to deliver.

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H2O2/HCl and heat-treated Ti-6Al-4V stimulates pre-osteoblast proliferation and differentiation

Cited by 41 publications

References 31 publications

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells

Biofunctionalization of a titanium surface with a nano-sawtooth structure regulates the behavior of rat bone marrow mesenchymal stem cells

Self-assembled rosette nanotubes encapsulate and slowly release dexamethasone

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