H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum

Amblard, Irène; Thauvin, Marion; Rampon, Christine; Quéguiner, Isabelle; Pak, Valeriy V.; Belousov, Vsevolod V.; Prochiantz, Alain; Volovitch, Michel; Joliot, Alain; Vriz, Sophie

doi:10.1038/s42003-020-01268-7

Cited by 18 publications

(29 citation statements)

References 42 publications

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“…It is worth mentioning that since H 2 O 2 is able to passively diffuse across cytoplasmic membrane, the observed effect of the antioxidants may be composite: it may be due to dismutation of H 2 O 2 inside or outside the cells. For instance, incubation of HeLa HyPer with the recombinant heme catalase, has shown to reduce H 2 O 2 concentration inside cells whereas it does not cross cells membrane [77].…”

Section: Resultsmentioning

confidence: 99%

“…Statistical significance was calculated using an ordinary one-way ANOVA followed by Tukey's multiple comparison test. Note that this assay was shown to provide a positive result with antioxidants not able to enter cells, such as purified catalase in the extracellular medium [77]. Indeed, H 2 O 2 is able to cross membranes and its consumption in the extracellular medium shifts the equilibrium and, in fine, consumes also H 2 O 2 inside cells, where it is measured by the Hyper probe.…”

Section: Assay On Hela Hyper Cellsmentioning

confidence: 91%

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Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models

Vincent

Thauvin

Quévrain

et al. 2021

Journal of Inorganic Biochemistry

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Oxidative stress that results from an imbalance between the concentrations of reactive species (RS) and antioxidant defenses is associated with many pathologies. Superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase are among the key enzymes that maintain the low nanomolar physiological concentrations of superoxide and hydrogen peroxide. The increase in the levels of these species and their progeny could have deleterious effects. In this context, chemists have developed SOD and CAT mimics to supplement them when cells are overwhelmed with oxidative stress. However, the beneficial activity of such molecules in cells depends not only on their intrinsic catalytic activities but also on their stability in biological context, their cell penetration and their cellular localization. We have employed cellular assays to characterize several compounds that possess SOD and CAT activities and have been frequently used in cellular and animal models. We used cellular assays that address SOD and CAT activities of the compounds. Finally, we determined the effect of compounds on the suppression of the inflammation in HT29-MD2 cells challenged by lipopolysaccharide. When the assay requires penetration inside cells, the SOD mimics Mn(III) mesotetrakis(N-(2′-nbutoxyethyl)pyridinium-2-yl)porphyrin (MnTnBuOE-2-PyP 5+ ) and Mn(II) dichloro [(4aR,13aR,17aR,21aR)-

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Section: Resultsmentioning

confidence: 99%

Section: Assay On Hela Hyper Cellsmentioning

confidence: 91%

Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models

Vincent

Thauvin

Quévrain

et al. 2021

Journal of Inorganic Biochemistry

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“…To the extent that these parameters are not gauged with very stringent measures (cell type-specific genetic control of oxidant source, scavenging of extracellular oxidants, and determination of redox targets in recipient cells) many studies have provided good evidence for [ 12 ], but not directly proven, paracrine redox signaling. In many studies, a loop of autocrine redox-stimulated release of paracrine soluble mediators could equally well account for the observations [ 101 ].…”

Section: Sod3 and Nox2 Interactions At The Macrophage Cell Surfacementioning

confidence: 99%

Novel and Converging Ways of NOX2 and SOD3 in Trafficking and Redox Signaling in Macrophages

Petersen

Poulsen²,

Matthiesen

et al. 2021

Antioxidants

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Macrophages and related tissue macrophage populations use the classical NADPH oxidase (NOX2) for the regulated production of superoxide and derived oxidants for pathogen combat and redox signaling. With an emphasis on macrophages, we discuss how sorting into secretory storage vesicles, agonist-responsive membrane trafficking, and segregation into sphingolipid and cholesterol-enriched microdomains (lipid rafts) determine the subcellular distribution and spatial organization of NOX2 and superoxide dismutase-3 (SOD3). We discuss how inflammatory activation of macrophages, in part through small GTPase Rab27A/B regulation of the secretory compartments, mediates the coalescence of these two proteins on the cell surface to deliver a focalized hydrogen peroxide output. In interplay with membrane-embedded oxidant transporters and redox sensitive target proteins, this arrangement allows for the autocrine and paracrine signaling, which govern macrophage activation states and transcriptional programs. By discussing examples of autocrine and paracrine redox signaling, we highlight why formation of spatiotemporal microenvironments where produced superoxide is rapidly converted to hydrogen peroxide and conveyed immediately to reach redox targets in proximal vicinity is required for efficient redox signaling. Finally, we discuss the recent discovery of macrophage-derived exosomes as vehicles of NOX2 holoenzyme export to other cells.

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“…It does so by directly regulating the formation of a morphogen (Engrailed) gradient. [23] In this review, we will focus on the modulatory and instructional roles of ROS on development and maintenance of epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Reactive oxygen species (ROS) constitute an additional player in regulating epithelial development

Hebbar

Knust

2021

BioEssays

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Reactive oxygen species (ROS) are highly reactive molecules produced in cells. So far, they have mostly been connected to diseases and pathological conditions. More recent results revealed a somewhat unexpected role of ROS in control of developmental processes. In this review, we elaborate on ROS in development, focussing on their connection to epithelial tissue morphogenesis. After briefly summarising unique characteristics of epithelial cells, we present some characteristic features of ROS species, their production and targets, with a focus on proteins important for epithelial development and function. Finally, we provide examples of regulation of epithelial morphogenesis by ROS, and also of developmental genes that regulate the overall redox status. We conclude by discussing future avenues of research that will further elucidate ROS regulation in epithelial development.

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H2O2 and Engrailed 2 paracrine activity synergize to shape the zebrafish optic tectum

Cited by 18 publications

References 42 publications

Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models

Evaluation of the compounds commonly known as superoxide dismutase and catalase mimics in cellular models

Novel and Converging Ways of NOX2 and SOD3 in Trafficking and Redox Signaling in Macrophages

Reactive oxygen species (ROS) constitute an additional player in regulating epithelial development

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