H2B ubiquitination: Conserved molecular mechanism, diverse physiologic functions of the E3 ligase during meiosis

Wang, Liying; Cao, Chunwei; Wang, Fang; Zhao, Jianguo; Li, Wei

doi:10.1080/19491034.2017.1330237

Cited by 23 publications

(16 citation statements)

References 42 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we obtained all exon sequences of the porcine RNF20 gene by sequence analysis of 10 PCR amplicons. Bioinformatic analysis based on predicted amino acid sequences revealed the high conservation of RNF20 across multiple species, which is consistent with previous reports [22].…”

Section: Discussionsupporting

confidence: 91%

Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene

Zhao

Yang

Wang

et al. 2020

Animals

View full text Add to dashboard Cite

Fat deposition is considered an economically important trait in pig breeding programs. Ring finger protein 20 (RNF20), an E3 ubiquitin protein ligase, has been shown to be closely involved in adipogenesis in mice, suggesting its conserved role in pigs. In this study, we obtained the exon sequences of the porcine RNF20 gene and characterized its molecular sequence. The porcine RNF20 gene contains 20 exons that encode 975 amino acids, and its RING domain is highly conserved across different species. Western blot analysis revealed that RNF20 was widely expressed, especially in various fat depots, and the level of H2B monoubiquitination (H2Bub) was highly consistent. Eight potential SNPs were detected by sequencing pooled PCR fragments. PCR–RFLP was developed to detect a single nucleotide polymorphism (A-1027G) in exon 1, and the allele frequency differences were examined in four pig breeds. The G allele was predominant in these pigs. Association analysis between (A-1027G) and the backfat thickness of three commercial pig breeds was performed, but no significant association was found. Taken together, these results enabled us to undertake the molecular characterization, expression profiling, and SNP analysis of the porcine RNF20 gene.

show abstract

Section: Discussionsupporting

confidence: 91%

Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene

Zhao

Yang

Wang

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…Sequence-specific DNA binding proteins, such as the Atf-Pcr1 heterodimer, Bas1, and Prdm9, regulate hotspots [ 3 , 5 – 7 ]. They trigger directly or indirectly posttranslational modifications (PTMs) of histones that help position recombination through the modulation of chromatin structure [ 8 – 11 ]. Individual species can have many hotspot-activating protein–DNA complexes [ 12 – 15 ], and to the extent tested, their regulatory functions are conserved in other species [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot

Storey

Wang

Protacio

et al. 2018

Epigenetics & Chromatin

View full text Add to dashboard Cite

BackgroundMeiotic recombination hotspots control the frequency and distribution of Spo11 (Rec12)-initiated recombination in the genome. Recombination occurs within and is regulated in part by chromatin structure, but relatively few of the many chromatin remodeling factors and histone posttranslational modifications (PTMs) have been interrogated for a role in the process.ResultsWe developed a chromatin affinity purification and mass spectrometry-based approach to identify proteins and histone PTMs that regulate recombination hotspots. Small (4.2 kbp) minichromosomes (MiniCs) bearing the fission yeast ade6-M26 hotspot or a basal recombination control were purified approximately 100,000-fold under native conditions from meiosis; then, associated proteins and histone PTMs were identified by mass spectrometry. Proteins and PTMs enriched at the hotspot included known regulators (Atf1, Pcr1, Mst2, Snf22, H3K14ac), validating the approach. The abundance of individual histones varied dynamically during meiotic progression in hotspot versus basal control MiniCs, as did a subset of 34 different histone PTMs, implicating these as potential regulators. Measurements of basal and hotspot recombination in null mutants confirmed that additional, hotspot-enriched proteins are bona fide regulators of hotspot activation within the genome. These chromatin-mediated regulators include histone H2A-H2B and H3-H4 chaperones (Nap1, Hip1/Hir1), subunits of the Ino80 complex (Arp5, Arp8), a DNA helicase/E3 ubiquitin ligase (Rrp2), components of a Swi2/Snf2 family remodeling complex (Swr1, Swc2), and a nucleosome evictor (Fft3/Fun30).ConclusionsOverall, our findings indicate that a remarkably diverse collection of chromatin remodeling factors and histone PTMs participate in designating where meiotic recombination occurs in the genome, and they provide new insight into molecular mechanisms of the process.Electronic supplementary materialThe online version of this article (10.1186/s13072-018-0233-x) contains supplementary material, which is available to authorized users.

show abstract

“…In addition to its role in gene expression (see later), H2B ubiquitination has also been related to the formation of DSBs [94]. The ubiquitination of H2B leads to chromatin relaxation, which, in the context of meiosis, enables the recruitment of several DSB repair factors to their proper positions at hotspots [94][95][96]. As a matter of fact, the involvement of H2Bub in chromatin relaxation is evolutionarily conserved from yeast to mammals [95].…”

Section: H2b Ubiquitination Is Important For Dsb Formationmentioning

confidence: 99%

“…The ubiquitination of H2B leads to chromatin relaxation, which, in the context of meiosis, enables the recruitment of several DSB repair factors to their proper positions at hotspots [94][95][96]. As a matter of fact, the involvement of H2Bub in chromatin relaxation is evolutionarily conserved from yeast to mammals [95]. Notably, the Rad6 homolog in mammals, HR6B, has been related to defects in male fertility when it is depleted in mice, due to errors during spermatogenesis.…”

Section: H2b Ubiquitination Is Important For Dsb Formationmentioning

confidence: 99%

Sharing Marks: H3K4 Methylation and H2B Ubiquitination as Features of Meiotic Recombination and Transcription

Serrano-Quílez

Roig-Soucase

Rodrı́guez-Navarro

2020

IJMS

View full text Add to dashboard Cite

Meiosis is a specialized cell division that gives raise to four haploid gametes from a single diploid cell. During meiosis, homologous recombination is crucial to ensure genetic diversity and guarantee accurate chromosome segregation. Both the formation of programmed meiotic DNA double-strand breaks (DSBs) and their repair using homologous chromosomes are essential and highly regulated pathways. Similar to other processes that take place in the context of chromatin, histone posttranslational modifications (PTMs) constitute one of the major mechanisms to regulate meiotic recombination. In this review, we focus on specific PTMs occurring in histone tails as driving forces of different molecular events, including meiotic recombination and transcription. In particular, we concentrate on the influence of H3K4me3, H2BK123ub, and their corresponding molecular machineries that write, read, and erase these histone marks. The Spp1 subunit within the Complex of Proteins Associated with Set1 (COMPASS) is a critical regulator of H3K4me3-dependent meiotic DSB formation. On the other hand, the PAF1c (RNA polymerase II associated factor 1 complex) drives the ubiquitination of H2BK123 by Rad6-Bre1. We also discuss emerging evidence obtained by cryo-electron microscopy (EM) structure determination that has provided new insights into how the “cross-talk” between these two marks is accomplished.

show abstract

H2B ubiquitination: Conserved molecular mechanism, diverse physiologic functions of the E3 ligase during meiosis

Cited by 23 publications

References 42 publications

Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene

Molecular Characterization, Expression Profiling, and SNP Analysis of the Porcine RNF20 Gene

Chromatin-mediated regulators of meiotic recombination revealed by proteomics of a recombination hotspot

Sharing Marks: H3K4 Methylation and H2B Ubiquitination as Features of Meiotic Recombination and Transcription

Contact Info

Product

Resources

About